Microfluidic device having an immobilized pH gradient and page gels for protein separation and analysis
Disclosed is a novel microfluidic device enabling on-chip implementation of a two-dimensional separation methodology. Previously disclosed microscale immobilized pH gradients (IPG) are combined with perpendicular polyacrylamide gel electrophoresis (PAGE) microchannels to achieve orthogonal separations of biological samples. Device modifications enable inclusion of sodium dodecyl sulfate (SDS) in the second dimension. The device can be fabricated to use either continuous IPG gels, or the microscale isoelectric fractionation membranes we have also previously disclosed, for the first dimension. The invention represents the first all-gel two-dimensional separation microdevice, with significantly higher resolution power over existing devices.
- Research Organization:
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC04-94AL85000
- Assignee:
- Sandia Corporation (Albuquerque, NM)
- Patent Number(s):
- 8,728,290
- Application Number:
- 13/667,254
- OSTI ID:
- 1132269
- Resource Relation:
- Patent File Date: 2012 Nov 02
- Country of Publication:
- United States
- Language:
- English
Polyacrylamide gel plugs enabling 2-D microfluidic protein separationsvia isoelectric focusing and multiplexed sodium dodecyl sulfate gel electrophoresis
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journal | June 2008 |
On-Chip Isoelectric Focusing Using Photopolymerized Immobilized pH Gradients
|
journal | May 2008 |
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