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Title: Three-Dimensional Orientation Determination of Stationary Anisotropic Nanoparticles with Sub-Degree Precision under Total Internal Reflection Scattering Microscopy

Single-particle and single-molecule orientation determination plays a vital role in deciphering nanoscale motion in complex environments. Previous attempts to determine the absolute three-dimensional orientation of anisotropic particles rely on subjective pattern matching and are inherently plagued by high degrees of uncertainty. Herein, we describe a method utilizing total internal reflection scattering microscopy to determine the 3D orientation of gold nanorods with subdegree uncertainty. The method is then applied to the biologically relevant system of microtubule cargo loading. Finally, we demonstrate the method holds potential for identifying single particles versus proximate neighbors within the diffraction limited area.
Publication Date:
OSTI Identifier:
Report Number(s):
Journal ID: ISSN 1530--6984
DOE Contract Number:
Resource Type:
Journal Article
Resource Relation:
Journal Name: Nano Letters; Journal Volume: 13; Journal Issue: 11
American Chemical Society
Research Org:
Ames Laboratory (AMES), Ames, IA (United States)
Sponsoring Org:
USDOE Office of Science (SC)
Country of Publication:
United States
77 NANOSCIENCE AND NANOTECHNOLOGY; Total internal reflection scattering (TIRS), single-particle, orientation determination, microtubule cargo, localized surface plasmon resonance