Metabolic engineering of Thermoanaerobacterium saccharolyticum for n-butanol production

Journal Article · Wed Jan 01 00:00:00 EST 2014 · Metabolic Engineering

DOI:https://doi.org/10.1016/j.ymben.2013.10.012· OSTI ID:1126961

Bhandiwad, Ashwini ^[1]; Shaw, A Joe ^[2]; Guss, Adam M ^[3]; Guseva, Anna ^[1]; Lynd, Lee R ^[1]

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1. Thayer School of Engineering at Dartmouth
2. Mascoma Corporation
3. ORNL

The thermophilic anaerobe Thermoanaerobacterium saccharolyticum JW/SL-YS485 was investigated as a host for n-butanol production. A systematic approach was taken to demonstrate functionality of heterologous components of the clostridial n-butanol pathway via gene expression and enzymatic activity assays in this organism. Subsequently, integration of the entire pathway in the wild-type strain resulted in n-butanol production of 0.85 g/L from 10 g/L xylose, corresponding to 21% of the theoretical maximum yield. We were unable to integrate the n-butanol pathway in strains lacking the ability to produce acetate, despite the theoretical overall redox neutrality of n-butanol formation. However, integration of the n-butanol pathway in lactate deficient strains resulted in n-butanol production of 1.05 g/L from 10 g/L xylose, corresponding to 26% of the theoretical maximum.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: USDOE Office of Science (SC)
• DOE Contract Number: DE-AC05-00OR22725
• OSTI ID: 1126961
• Journal Information: Metabolic Engineering, Vol. 21; ISSN 1096-7176
• Country of Publication: United States
• Language: English