Global SAXS Data Analysis for Multilamellar Vesicles: Evolution of the Scattering Density Profile (SDP) Model
Abstract
The highly successful scattering density profile (SDP) model, used to jointly analyze small-angle X-ray and neutron scattering data from unilamellar vesicles, has been adapted for use with data from fully hydrated, liquid crystalline multilamellar vesicles (MLVs). Using a genetic algorithm, this new method is capable of providing high-resolution structural information, as well as determining bilayer elastic bending fluctuations from standalone X-ray data. Structural parameters such as bilayer thickness and area per lipid were determined for a series of saturated and unsaturated lipids, as well as binary mixtures with cholesterol. The results are in good agreement with previously reported SDP data, which used both neutron and X-ray data. The inclusion of deuterated and non-deuterated MLV neutron data in the analysis improved the lipid backbone information but did not improve, within experimental error, the structural data regarding bilayer thickness and area per lipid.
- Authors:
-
- University of Graz, Institute of Molecular Biosciences, Austria
- ORNL
- University of Leeds, UK
- Graz University of Technology
- Atomic Energy of Canada Limited (AECL), Canadian Neutron Beam Centre (CNBC) and Comenius University,
- Publication Date:
- Research Org.:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Spallation Neutron Source (SNS)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1117462
- DOE Contract Number:
- DE-AC05-00OR22725
- Resource Type:
- Journal Article
- Journal Name:
- Journal of Applied Crystallography
- Additional Journal Information:
- Journal Volume: 47; Journal Issue: 1; Journal ID: ISSN 1600--5767
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Heftberger, Peter, Kollmitzer, Benjamin, Heberle, Frederick A, Pan, Jianjun, Rappolt, Michael, Amenitsch, Heinz, Kucerka, Norbert, Katsaras, John, and Pabst, georg. Global SAXS Data Analysis for Multilamellar Vesicles: Evolution of the Scattering Density Profile (SDP) Model. United States: N. p., 2014.
Web. doi:10.1107/S1600576713029798.
Heftberger, Peter, Kollmitzer, Benjamin, Heberle, Frederick A, Pan, Jianjun, Rappolt, Michael, Amenitsch, Heinz, Kucerka, Norbert, Katsaras, John, & Pabst, georg. Global SAXS Data Analysis for Multilamellar Vesicles: Evolution of the Scattering Density Profile (SDP) Model. United States. https://doi.org/10.1107/S1600576713029798
Heftberger, Peter, Kollmitzer, Benjamin, Heberle, Frederick A, Pan, Jianjun, Rappolt, Michael, Amenitsch, Heinz, Kucerka, Norbert, Katsaras, John, and Pabst, georg. 2014.
"Global SAXS Data Analysis for Multilamellar Vesicles: Evolution of the Scattering Density Profile (SDP) Model". United States. https://doi.org/10.1107/S1600576713029798.
@article{osti_1117462,
title = {Global SAXS Data Analysis for Multilamellar Vesicles: Evolution of the Scattering Density Profile (SDP) Model},
author = {Heftberger, Peter and Kollmitzer, Benjamin and Heberle, Frederick A and Pan, Jianjun and Rappolt, Michael and Amenitsch, Heinz and Kucerka, Norbert and Katsaras, John and Pabst, georg},
abstractNote = {The highly successful scattering density profile (SDP) model, used to jointly analyze small-angle X-ray and neutron scattering data from unilamellar vesicles, has been adapted for use with data from fully hydrated, liquid crystalline multilamellar vesicles (MLVs). Using a genetic algorithm, this new method is capable of providing high-resolution structural information, as well as determining bilayer elastic bending fluctuations from standalone X-ray data. Structural parameters such as bilayer thickness and area per lipid were determined for a series of saturated and unsaturated lipids, as well as binary mixtures with cholesterol. The results are in good agreement with previously reported SDP data, which used both neutron and X-ray data. The inclusion of deuterated and non-deuterated MLV neutron data in the analysis improved the lipid backbone information but did not improve, within experimental error, the structural data regarding bilayer thickness and area per lipid.},
doi = {10.1107/S1600576713029798},
url = {https://www.osti.gov/biblio/1117462},
journal = {Journal of Applied Crystallography},
issn = {1600--5767},
number = 1,
volume = 47,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 2014},
month = {Wed Jan 01 00:00:00 EST 2014}
}