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Title: Metabolic Profiling Directly from the Petri Dish Using Nanospray Desorption Electrospray Ionization Imaging Mass Spectrometry

Abstract

Understanding molecular interaction pathways in complex biological systems constitutes a treasure trove of knowledge that might facilitate the specific, chemical manipulation of the countless microbiological systems that occur throughout our world. However, there is a lack of methodologies that allow the direct investigation of chemical gradients and interactions in living biological systems, in real time. Here, we report the use of nanospray desorption electrospray ionization (nanoDESI) imaging mass spectrometry for in vivo metabolic profiling of living bacterial colonies directly from the Petri dish with absolutely no sample preparation needed. Using this technique, we investigated single colonies of Shewanella oneidensis MR-1, Bacillus subtilis 3610, and Streptomyces coelicolor A3(2) as well as a mixed biofilm of S. oneidensis MR-1 and B. subtilis 3610. Data from B. subtilis 3610 and S. coelicolor A3(2) provided a means of validation for the method while data from S. oneidensis MR-1 and the mixed biofilm showed a wide range of compounds that this bacterium uses for the dissimilatory reduction of extracellular metal oxides, including riboflavin, iron-bound heme and heme biosynthetic intermediates, and the siderophore putrebactin.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1115831
Report Number(s):
PNNL-SA-99934
44679
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Analytical Chemistry, 85(21):10385-10391
Additional Journal Information:
Journal Name: Analytical Chemistry, 85(21):10385-10391
Country of Publication:
United States
Language:
English
Subject:
Environmental Molecular Sciences Laboratory

Citation Formats

Watrous, Jeramie D., Roach, Patrick J., Heath, Brandi S., Alexandrov, Theodore, Laskin, Julia, and Dorrestein, Pieter C. Metabolic Profiling Directly from the Petri Dish Using Nanospray Desorption Electrospray Ionization Imaging Mass Spectrometry. United States: N. p., 2013. Web. doi:10.1021/ac4023154.
Watrous, Jeramie D., Roach, Patrick J., Heath, Brandi S., Alexandrov, Theodore, Laskin, Julia, & Dorrestein, Pieter C. Metabolic Profiling Directly from the Petri Dish Using Nanospray Desorption Electrospray Ionization Imaging Mass Spectrometry. United States. https://doi.org/10.1021/ac4023154
Watrous, Jeramie D., Roach, Patrick J., Heath, Brandi S., Alexandrov, Theodore, Laskin, Julia, and Dorrestein, Pieter C. 2013. "Metabolic Profiling Directly from the Petri Dish Using Nanospray Desorption Electrospray Ionization Imaging Mass Spectrometry". United States. https://doi.org/10.1021/ac4023154.
@article{osti_1115831,
title = {Metabolic Profiling Directly from the Petri Dish Using Nanospray Desorption Electrospray Ionization Imaging Mass Spectrometry},
author = {Watrous, Jeramie D. and Roach, Patrick J. and Heath, Brandi S. and Alexandrov, Theodore and Laskin, Julia and Dorrestein, Pieter C.},
abstractNote = {Understanding molecular interaction pathways in complex biological systems constitutes a treasure trove of knowledge that might facilitate the specific, chemical manipulation of the countless microbiological systems that occur throughout our world. However, there is a lack of methodologies that allow the direct investigation of chemical gradients and interactions in living biological systems, in real time. Here, we report the use of nanospray desorption electrospray ionization (nanoDESI) imaging mass spectrometry for in vivo metabolic profiling of living bacterial colonies directly from the Petri dish with absolutely no sample preparation needed. Using this technique, we investigated single colonies of Shewanella oneidensis MR-1, Bacillus subtilis 3610, and Streptomyces coelicolor A3(2) as well as a mixed biofilm of S. oneidensis MR-1 and B. subtilis 3610. Data from B. subtilis 3610 and S. coelicolor A3(2) provided a means of validation for the method while data from S. oneidensis MR-1 and the mixed biofilm showed a wide range of compounds that this bacterium uses for the dissimilatory reduction of extracellular metal oxides, including riboflavin, iron-bound heme and heme biosynthetic intermediates, and the siderophore putrebactin.},
doi = {10.1021/ac4023154},
url = {https://www.osti.gov/biblio/1115831}, journal = {Analytical Chemistry, 85(21):10385-10391},
number = ,
volume = ,
place = {United States},
year = {Tue Nov 05 00:00:00 EST 2013},
month = {Tue Nov 05 00:00:00 EST 2013}
}