Absorption Mode FT-ICR Mass Spectrometry Imaging

Smith, Donald F.; Kilgour, David P.; Konijnenburg, Marco; O'Connor, Peter B.; Heeren, Ronald M.

doi:10.1021/ac403039t

Title: Absorption Mode FT-ICR Mass Spectrometry Imaging

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Abstract

Fourier transform ion cyclotron resonance mass spectrometry offers the highest mass resolving power for molecular imaging experiments. This high mass resolving power ensures that closely spaced peaks at the same nominal mass are resolved for proper image generation. Typically higher magnetic fields are used to increase mass resolving power. However, a gain in mass resolving power can also be realized by phase correction of the data for absorption mode display. In addition to mass resolving power, absorption mode offers higher mass accuracy and signal-to-noise ratio over the conventional magnitude mode. Here we present the first use of absorption mode for Fourier transform ion cyclotron resonance mass spectrometry imaging. The Autophaser algorithm is used to phase correct each spectrum (pixel) in the image and then these parameters are used by the Chameleon work-flow based data processing software to generate absorption mode ?Datacubes? for image and spectral viewing. Absorption mode reveals new mass and spatial features that are not resolved in magnitude mode and results in improved selected ion image contrast.

Authors:: Smith, Donald F.; Kilgour, David P.; Konijnenburg, Marco; O'Connor, Peter B.; Heeren, Ronald M.

Publication Date:: Tue Dec 03 00:00:00 EST 2013

Research Org.:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1115827

DOE Contract Number:: AC05-76RL01830

Resource Type:: Journal Article

Journal Name:: Analytical Chemistry, 85(23):11180-11184

Additional Journal Information:: Journal Name: Analytical Chemistry, 85(23):11180-11184

Country of Publication:: United States

Language:: English

Subject:: Environmental Molecular Sciences Laboratory

Citation Formats


                    Smith, Donald F., Kilgour, David P., Konijnenburg, Marco, O'Connor, Peter B., and Heeren, Ronald M. Absorption Mode FT-ICR Mass Spectrometry Imaging.  United States: N. p., 2013. 
        Web.  doi:10.1021/ac403039t.

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                    Smith, Donald F., Kilgour, David P., Konijnenburg, Marco, O'Connor, Peter B., & Heeren, Ronald M. Absorption Mode FT-ICR Mass Spectrometry Imaging.  United States.  https://doi.org/10.1021/ac403039t

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                    Smith, Donald F., Kilgour, David P., Konijnenburg, Marco, O'Connor, Peter B., and Heeren, Ronald M. 2013.  
        "Absorption Mode FT-ICR Mass Spectrometry Imaging".  United States.  https://doi.org/10.1021/ac403039t.

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@article{osti_1115827,

  title        = {Absorption Mode FT-ICR Mass Spectrometry Imaging},

  author       = {Smith, Donald F. and Kilgour, David P. and Konijnenburg, Marco and O'Connor, Peter B. and Heeren, Ronald M.},

  abstractNote = {Fourier transform ion cyclotron resonance mass spectrometry offers the highest mass resolving power for molecular imaging experiments. This high mass resolving power ensures that closely spaced peaks at the same nominal mass are resolved for proper image generation. Typically higher magnetic fields are used to increase mass resolving power. However, a gain in mass resolving power can also be realized by phase correction of the data for absorption mode display. In addition to mass resolving power, absorption mode offers higher mass accuracy and signal-to-noise ratio over the conventional magnitude mode. Here we present the first use of absorption mode for Fourier transform ion cyclotron resonance mass spectrometry imaging. The Autophaser algorithm is used to phase correct each spectrum (pixel) in the image and then these parameters are used by the Chameleon work-flow based data processing software to generate absorption mode ?Datacubes? for image and spectral viewing. Absorption mode reveals new mass and spatial features that are not resolved in magnitude mode and results in improved selected ion image contrast.},

  doi          = {10.1021/ac403039t},

  url          = {https://www.osti.gov/biblio/1115827},
  journal      = {Analytical Chemistry, 85(23):11180-11184},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Dec 03 00:00:00 EST 2013},

  month        = {Tue Dec 03 00:00:00 EST 2013}

}

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