Boron neutron capture therapy (BNCT) for liver metastasis in an experimental model: dose–response at five-week follow-up based on retrospective dose assessment in individual rats

Journal Article · Fri Nov 01 00:00:00 EDT 2013 · Radiation and Environmental Biophysics

DOI:https://doi.org/10.1007/s00411-013-0490-9· OSTI ID:1111036

Pozzi, Emiliano C. C.; Trivilin, Veronica A.; Colombo, Lucas L.; Hughes, Andrea Monti; Thorp, Silvia I.; Cardoso, Jorge E.; Garabalino, Marcel A.; Molinari, Ana J.; Heber, Elisa M.; Curotto, Paula; Miller, Marcelo; Itoiz, Maria E.; Aromando, Romina F.; Nigg, David W.; Schwint, Amanda E.

Boron neutron capture therapy (BNCT) was proposed for untreatable colorectal liver metastases. Employing an experimental model of liver metastases in rats, we recently demonstrated that BNCT mediated by boronophenylalanine (BPA-BNCT) at 13 Gy prescribed to tumor is therapeutically useful at 3-week follow-up. The aim of the present study was to evaluate dose–response at 5-week follow-up, based on retrospective dose assessment in individual rats. BDIX rats were inoculated with syngeneic colon cancer cells DHD/K12/TRb. Tumor-bearing animals were divided into three groups: BPA-BNCT (n = 19), Beam only (n = 8) and Sham (n = 7) (matched manipulation, no treatment). For each rat, neutron flux was measured in situ and boron content was measured in a pre-irradiation blood sample for retrospective individual dose assessment. For statistical analysis (ANOVA), individual data for the BPA-BNCT group were pooled according to absorbed tumor dose, BPA-BNCT I: 4.5–8.9 Gy and BPA-BNCT II: 9.2–16 Gy. At 5 weeks post-irradiation, the tumor surface area post-treatment/pre-treatment ratio was 12.2 +/- 6.6 for Sham, 7.8 +/- 4.1 for Beam only, 4.4 +/- 5.6 for BPA-BNCT I and 0.45 +/- 0.20 for BPA-BNCT II; tumor nodule weight was 750 +/- 480 mg for Sham, 960 +/- 620 mg for Beam only, 380 +/- 720 mg for BPA-BNCT I and 7.3 +/- 5.9 mg for BPA-BNCT II. The BPA-BNCT II group exhibited statistically significant tumor control with no contributory liver toxicity. Potential threshold doses for tumor response and significant tumor control were established at 6.1 and 9.2 Gy, respectively.

• Research Organization: Idaho National Lab. (INL), Idaho Falls, ID (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: DE-AC07-05ID14517
• OSTI ID: 1111036
• Report Number(s): INL/JOU-13-30871
• Journal Information: Radiation and Environmental Biophysics, Vol. 52, Issue 4; ISSN 0301--634X
• Country of Publication: United States
• Language: English

Related Subjects

99 GENERAL AND MISCELLANEOUS
BDIX rats
BNCT
Boron neutron capture therapy
DHD/K12/TRb cells
liver metastasis
liver metastasis experimental model