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Title: Switchgrass ubiquitin promoter (PVUBI2) and uses thereof

Patent ·
OSTI ID:1109474

The subject application provides polynucleotides, compositions thereof and methods for regulating gene expression in a plant. Polynucleotides disclosed herein comprise novel sequences for a promoter isolated from Panicum virgatum (switchgrass) that initiates transcription of an operably linked nucleotide sequence. Thus, various embodiments of the invention comprise the nucleotide sequence of SEQ ID NO: 2 or fragments thereof comprising nucleotides 1 to 692 of SEQ ID NO: 2 that are capable of driving the expression of an operably linked nucleic acid sequence.

Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC05-00OR22725
Assignee:
University of Tennessee Research Foundation (Knoxville, TN)
Patent Number(s):
8,604,276
Application Number:
12/797,248
OSTI ID:
1109474
Country of Publication:
United States
Language:
English

References (12)

Maize polyubiquitin genes: structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation journal February 1992
Isolation of a Polyubiquitin Promoter and Its Expression in Transgenic Potato Plants journal December 1995
Comparative expression analysis of two sugarcane polyubiquitin promoters and flanking sequences in transgenic plants journal January 2003
Structure and expression of sunflower ubiquitin genes journal September 1991
Characterization of a polyubiquitin gene from Arabidopsis thaliana journal August 1988
Nucleotide Sequences And Corresponding Polypeptides Conferring Modulated Plant Characteristics patent-application July 2011
Rice ubiquitin-derived promoters patent March 2003
Comparative Genomics in Switchgrass Using 61,585 High-Quality Expressed Sequence Tags journal January 2008
Structure and promoter activity of a stress and developmentally regulated polyubiquitin-encoding gene of Nicotiana tabacum journal October 1994
Polyubiquitin gene expression and structural properties of the ubi4-2 gene in Petroselinum crispum journal February 1993
Activity of a maize ubiquitin promoter in transgenic rice journal November 1993
PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences journal January 2002