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Title: Methods for detection of methyl-CpG dinucleotides

The invention provides methods for enriching methyl-CpG sequences from a DNA sample. The method makes use of conversion of cytosine residues to uracil under conditions in which methyl-cytosine residues are preserved. Additional methods of the invention enable to preservation of the context of me-CpG dinucleotides. The invention also provides a recombinant, full length and substantially pure McrA protein (rMcrA) for binding and isolation of DNA fragments containing the sequence 5'-C.sup.MeCpGG-3'. Methods for making and using the rMcrA protein, and derivatives thereof are provided.
Authors:
Publication Date:
OSTI Identifier:
1109096
Report Number(s):
8,592,185
13/565,954
DOE Contract Number:
AC02-98CH10886
Resource Type:
Patent
Research Org:
BNL (Brookhaven National Laboratory (BNL), Upton, NY (United States))
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES