Increasing Confidence of LC-MS Identifications by Utilizing Ion Mobility Spectrometry
Ion mobility spectrometry in conjunction with liquid chromatography separations and mass spectrometry offers a range of new possibilities for analyzing complex biological samples. To fully utilize the information obtained from these three measurement dimensions, informatics tools based on the accurate mass and time tag methodology were modified to incorporate ion mobility spectrometry drift times for peptides observed in human serum. A reference human serum database was created using 12,139 peptides, tracking the monoisotopic mass, liquid chromatography normalized elution time, and ion mobility spectrometry drift time(s) for each peptide. We demonstrate that the use of three dimensions for peak matching during the peptide identification process resulted in increased numbers of identifications and lower false discovery rates relative to the use of only the mass and normalized elution time dimensions.
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 1108126
- Report Number(s):
- PNNL-SA-95461; 47418; KP1601010
- Journal Information:
- International Journal of Mass Spectrometry, 354-355:312-317, Journal Name: International Journal of Mass Spectrometry, 354-355:312-317
- Country of Publication:
- United States
- Language:
- English
Similar Records
Estimating Probabilities of Peptide Database Identifications to LC-FTICR-MS Observations
Using Skyline to Analyze Data-Containing Liquid Chromatography, Ion Mobility Spectrometry, and Mass Spectrometry Dimensions