Nanoplasmonic molecular ruler for nuclease activity and DNA footprinting

Chen, Fanqing Frank; Liu, Gang L; Lee, Luke P

Title: Nanoplasmonic molecular ruler for nuclease activity and DNA footprinting

Patent · Tue Oct 29 00:00:00 EDT 2013

OSTI ID:1107635

Chen, Fanqing Frank; Liu, Gang L; Lee, Luke P

This invention provides a nanoplasmonic molecular ruler, which can perform label-free and real-time monitoring of nucleic acid (e.g., DNA) length changes and perform nucleic acid footprinting. In various embodiments the ruler comprises a nucleic acid attached to a nanoparticle, such that changes in the nucleic acid length are detectable using surface plasmon resonance. The nanoplasmonic ruler provides a fast and convenient platform for mapping nucleic acid-protein interactions, for nuclease activity monitoring, and for other footprinting related methods.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC02-05CH11231

Assignee:: The Regents of the University of California (Oakland, CA)

Patent Number(s):: 8,569,468

Application Number:: 12/440,579

OSTI ID:: 1107635

Country of Publication:: United States

Language:: English

References (15)

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