Diffusion in biofilms respiring on electrodes
Abstract
The goal of this study was to measure spatially and temporally resolved effective diffusion coefficients (De) in biofilms respiring on electrodes. Two model electrochemically active biofilms, Geobacter sulfurreducens PCA and Shewanella oneidensis MR-1, were investigated. A novel nuclear magnetic resonance microimaging perfusion probe capable of simultaneous electrochemical and pulsed-field gradient nuclear magnetic resonance (PFG-NMR) techniques was used. PFG-NMR allowed for noninvasive, nondestructive, high spatial resolution in situ De measurements in living biofilms respiring on electrodes. The electrodes were polarized so that they would act as the sole terminal electron acceptor for microbial metabolism. We present our results as both two-dimensional De heat maps and surface-averaged relative effective diffusion coefficient (Drs) depth profiles. We found that (1) Drs decreases with depth in G. sulfurreducens biofilms, following a sigmoid shape; (2) Drs at a given location decreases with G. sulfurreducens biofilm age; (3) average De and Drs profiles in G. sulfurreducens biofilms are lower than those in S. oneidensis biofilms—the G. sulfurreducens biofilms studied here were on average 10 times denser than the S. oneidensis biofilms; and (4) halting the respiration of a G. sulfurreducens biofilm decreases the De values. Density, reflected by De, plays a major role in the extracellular electronmore »
- Authors:
-
- Washington State Univ., Pullman, WA (United States)
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Publication Date:
- Research Org.:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1094935
- Report Number(s):
- PNNL-SA-94190
Journal ID: ISSN 1754-5692; 34744; 47495; 400403309
- DOE Contract Number:
- AC05-76RL01830
- Resource Type:
- Journal Article
- Journal Name:
- Energy and Environmental Science
- Additional Journal Information:
- Journal Volume: 6; Journal Issue: 2; Journal ID: ISSN 1754-5692
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; SHEWANELLA-ONEIDENSIS MR-1; MICROBIAL FUEL-CELLS; GEOBACTER-SULFURREDUCENS BIOFILMS; WASTE-WATER TREATMENT; ELECTRICAL-CONDUCTIVITY; ELECTROACTIVE BIOFILMS; CONFOCAL MICROSCOPY; BACTERIAL NANOWIRES; MAGNETIC-RESONANCE; DESALINATION CELL; Environmental Molecular Sciences Laboratory
Citation Formats
Renslow, Ryan S., Babauta, Jerome T., Majors, Paul D., and Beyenal, Haluk. Diffusion in biofilms respiring on electrodes. United States: N. p., 2012.
Web. doi:10.1039/C2EE23394K.
Renslow, Ryan S., Babauta, Jerome T., Majors, Paul D., & Beyenal, Haluk. Diffusion in biofilms respiring on electrodes. United States. https://doi.org/10.1039/C2EE23394K
Renslow, Ryan S., Babauta, Jerome T., Majors, Paul D., and Beyenal, Haluk. 2012.
"Diffusion in biofilms respiring on electrodes". United States. https://doi.org/10.1039/C2EE23394K.
@article{osti_1094935,
title = {Diffusion in biofilms respiring on electrodes},
author = {Renslow, Ryan S. and Babauta, Jerome T. and Majors, Paul D. and Beyenal, Haluk},
abstractNote = {The goal of this study was to measure spatially and temporally resolved effective diffusion coefficients (De) in biofilms respiring on electrodes. Two model electrochemically active biofilms, Geobacter sulfurreducens PCA and Shewanella oneidensis MR-1, were investigated. A novel nuclear magnetic resonance microimaging perfusion probe capable of simultaneous electrochemical and pulsed-field gradient nuclear magnetic resonance (PFG-NMR) techniques was used. PFG-NMR allowed for noninvasive, nondestructive, high spatial resolution in situ De measurements in living biofilms respiring on electrodes. The electrodes were polarized so that they would act as the sole terminal electron acceptor for microbial metabolism. We present our results as both two-dimensional De heat maps and surface-averaged relative effective diffusion coefficient (Drs) depth profiles. We found that (1) Drs decreases with depth in G. sulfurreducens biofilms, following a sigmoid shape; (2) Drs at a given location decreases with G. sulfurreducens biofilm age; (3) average De and Drs profiles in G. sulfurreducens biofilms are lower than those in S. oneidensis biofilms—the G. sulfurreducens biofilms studied here were on average 10 times denser than the S. oneidensis biofilms; and (4) halting the respiration of a G. sulfurreducens biofilm decreases the De values. Density, reflected by De, plays a major role in the extracellular electron transfer strategies of electrochemically active biofilms.},
doi = {10.1039/C2EE23394K},
url = {https://www.osti.gov/biblio/1094935},
journal = {Energy and Environmental Science},
issn = {1754-5692},
number = 2,
volume = 6,
place = {United States},
year = {Thu Nov 15 00:00:00 EST 2012},
month = {Thu Nov 15 00:00:00 EST 2012}
}