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Title: Xylanases, nucleic acids encoding them and methods for making and using them

The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal .beta.-1,4-xylosidic linkages or endo-.beta.-1,4-glucanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.
Authors:
;
Publication Date:
OSTI Identifier:
1087879
Report Number(s):
8,486,680
12/681,604
DOE Contract Number:
1435-04-03-CA-70224
Resource Type:
Patent
Research Org:
DOEEE (USDOE Office of Energy Efficiency and Renewable Energy (EE) (United States))
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES