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Title: Simultaneous Single-Particle Superlocalization and Rotational Tracking

Abstract

Superlocalization of single molecules and nanoparticles has become an essential procedure to bring new insights into nanoscale structures and dynamics of biological systems. In the present study, superlocalization is combined with the newly introduced differential interference contrast (DIC) microscopy-based single-particle orientation and rotational tracking. The new technique overcomes the difficulty in localization of the antisymmetric DIC point spread function by using a dual-modality microscope configuration for simultaneous rotational tracking and localization of single gold nanorods with nanometer-scale precision. The new imaging setup has been applied to study the steric hindrance induced by relatively large cargos in the microtubule gliding assay and to track nanocargos in the crowded cellular environment. This technique has great potential in the study of biological processes where both localization and rotational information are required.

Authors:
 [1];  [2];  [1]
  1. Ames Laboratory
  2. North Carolina State University
Publication Date:
Research Org.:
Ames Lab., Ames, IA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1082461
Report Number(s):
IS-J 7944
DOE Contract Number:  
DE-AC02-07CH11358
Resource Type:
Journal Article
Journal Name:
ACS NANO
Additional Journal Information:
Journal Volume: 7; Journal Issue: 2
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; imaging; microscopy; superlocalization; gold nanorods; rotational tracking; kinesin

Citation Formats

Gu, Yan, Wang, Gufeng, and Fang, Ning. Simultaneous Single-Particle Superlocalization and Rotational Tracking. United States: N. p., 2013. Web. doi:10.1021/nn305640y.
Gu, Yan, Wang, Gufeng, & Fang, Ning. Simultaneous Single-Particle Superlocalization and Rotational Tracking. United States. https://doi.org/10.1021/nn305640y
Gu, Yan, Wang, Gufeng, and Fang, Ning. 2013. "Simultaneous Single-Particle Superlocalization and Rotational Tracking". United States. https://doi.org/10.1021/nn305640y.
@article{osti_1082461,
title = {Simultaneous Single-Particle Superlocalization and Rotational Tracking},
author = {Gu, Yan and Wang, Gufeng and Fang, Ning},
abstractNote = {Superlocalization of single molecules and nanoparticles has become an essential procedure to bring new insights into nanoscale structures and dynamics of biological systems. In the present study, superlocalization is combined with the newly introduced differential interference contrast (DIC) microscopy-based single-particle orientation and rotational tracking. The new technique overcomes the difficulty in localization of the antisymmetric DIC point spread function by using a dual-modality microscope configuration for simultaneous rotational tracking and localization of single gold nanorods with nanometer-scale precision. The new imaging setup has been applied to study the steric hindrance induced by relatively large cargos in the microtubule gliding assay and to track nanocargos in the crowded cellular environment. This technique has great potential in the study of biological processes where both localization and rotational information are required.},
doi = {10.1021/nn305640y},
url = {https://www.osti.gov/biblio/1082461}, journal = {ACS NANO},
number = 2,
volume = 7,
place = {United States},
year = {Wed Jan 30 00:00:00 EST 2013},
month = {Wed Jan 30 00:00:00 EST 2013}
}