Evaluation of the FilmArray® system for detection of Bacillus anthracis, Francisella tularensis, and Yersinia pestis

Seiner, Derrick R.; Colburn, Heather A.; Baird, Cheryl L.; Bartholomew, Rachel A.; Straub, Tim M.; Victry, Kristin D.; Hutchison, Janine R.; Valentine, Nancy B.; Bruckner-Lea, Cindy J.

doi:10.1111/jam.12107

Title: Evaluation of the FilmArray® system for detection of Bacillus anthracis, Francisella tularensis, and Yersinia pestis

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Abstract

To evaluate the sensitivity and specificity of the Idaho Technologies FilmArray® Biothreat Panel for the detection of Bacillus anthracis (Ba), Francisella tularensis (Ft), and Yersinia pestis (Yp) DNA, and demonstrate the detection of Ba spores. Methods and Results: DNA samples from Ba, Ft and Yp strains and near-neighbors, and live Ba spores were analyzed using the Biothreat Panel, a multiplexed PCR-based assay for 17 pathogens and toxins. Sensitivity studies with DNA suggest a limit of detection of 250 genome equivalents (GEs) per sample. Furthermore, the correct call of Ft, Yp or Bacillus species was made in 63 of 72 samples tested at 25 GE or less. With samples containing 25 Ba Sterne spores, at least one of the two possible Ba markers were identified in all samples tested. We observed no cross-reactivity with near-neighbor DNAs.

Authors:: Seiner, Derrick R.; Colburn, Heather A.; Baird, Cheryl L.; Bartholomew, Rachel A.; Straub, Tim M.; Victry, Kristin D.; Hutchison, Janine R.; Valentine, Nancy B.; Bruckner-Lea, Cindy J.

Publication Date:: Mon Apr 29 00:00:00 EDT 2013

Research Org.:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1079117

Report Number(s):: PNNL-SA-87496
400904120

DOE Contract Number:: AC05-76RL01830

Resource Type:: Journal Article

Journal Name:: Journal of Applied Microbiology, 114(4):992-1000

Additional Journal Information:: Journal Name: Journal of Applied Microbiology, 114(4):992-1000

Country of Publication:: United States

Language:: English

Subject:: Biodetection, PCR, hand portable, pathogens, bioterrorism, first responders, sample preparation

Citation Formats


                    Seiner, Derrick R., Colburn, Heather A., Baird, Cheryl L., Bartholomew, Rachel A., Straub, Tim M., Victry, Kristin D., Hutchison, Janine R., Valentine, Nancy B., and Bruckner-Lea, Cindy J. Evaluation of the FilmArray® system for detection of Bacillus anthracis, Francisella tularensis, and Yersinia pestis.  United States: N. p., 2013. 
        Web.  doi:10.1111/jam.12107.

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                    Seiner, Derrick R., Colburn, Heather A., Baird, Cheryl L., Bartholomew, Rachel A., Straub, Tim M., Victry, Kristin D., Hutchison, Janine R., Valentine, Nancy B., & Bruckner-Lea, Cindy J. Evaluation of the FilmArray® system for detection of Bacillus anthracis, Francisella tularensis, and Yersinia pestis.  United States.  https://doi.org/10.1111/jam.12107

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                    Seiner, Derrick R., Colburn, Heather A., Baird, Cheryl L., Bartholomew, Rachel A., Straub, Tim M., Victry, Kristin D., Hutchison, Janine R., Valentine, Nancy B., and Bruckner-Lea, Cindy J. 2013.  
        "Evaluation of the FilmArray® system for detection of Bacillus anthracis, Francisella tularensis, and Yersinia pestis".  United States.  https://doi.org/10.1111/jam.12107.

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@article{osti_1079117,

  title        = {Evaluation of the FilmArray® system for detection of Bacillus anthracis, Francisella tularensis, and Yersinia pestis},

  author       = {Seiner, Derrick R. and Colburn, Heather A. and Baird, Cheryl L. and Bartholomew, Rachel A. and Straub, Tim M. and Victry, Kristin D. and Hutchison, Janine R. and Valentine, Nancy B. and Bruckner-Lea, Cindy J.},

  abstractNote = {To evaluate the sensitivity and specificity of the Idaho Technologies FilmArray® Biothreat Panel for the detection of Bacillus anthracis (Ba), Francisella tularensis (Ft), and Yersinia pestis (Yp) DNA, and demonstrate the detection of Ba spores. Methods and Results: DNA samples from Ba, Ft and Yp strains and near-neighbors, and live Ba spores were analyzed using the Biothreat Panel, a multiplexed PCR-based assay for 17 pathogens and toxins. Sensitivity studies with DNA suggest a limit of detection of 250 genome equivalents (GEs) per sample. Furthermore, the correct call of Ft, Yp or Bacillus species was made in 63 of 72 samples tested at 25 GE or less. With samples containing 25 Ba Sterne spores, at least one of the two possible Ba markers were identified in all samples tested. We observed no cross-reactivity with near-neighbor DNAs.},

  doi          = {10.1111/jam.12107},

  url          = {https://www.osti.gov/biblio/1079117},
  journal      = {Journal of Applied Microbiology, 114(4):992-1000},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Mon Apr 29 00:00:00 EDT 2013},

  month        = {Mon Apr 29 00:00:00 EDT 2013}

}

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