The effect of deuteration on the structure of bacterial cellulose

Journal Article · Tue Jan 01 00:00:00 EST 2013 · carbohydrate research

DOI:https://doi.org/10.1016/j.carres.2013.04.009· OSTI ID:1078190

Bali, Garima ^[1]; Foston, Marcus ^[1]; O'Neill, Hugh Michael ^[2]; Evans, Barbara R ^[2]; He, Junhong ^[2]; Ragauskas, Arthur ^[1]

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1. Georgia Institute of Technology
2. ORNL

ABSTRACT In vivo generated deuterated bacterial cellulose, cultivated from 100% deuterated glycerol in D2O medium, was analyzed for deuterium incorporation by ionic liquid dissolution and 2H and 1H nuclear magnetic resonance (NMR). A solution NMR method of the dissolved cellulose was used to determine that this bacterial cellulose had 85 % deuterium incorporation. Acetylation and 1H and 2H NMR of deuterated bacterial cellulose indicated near equal deuteration at all sites of the glucopyranosyl ring except C-6 which was partly deuterated. Despite the high level of deuterium incorporation there were no significant differences in the molecular and morphological properties were observed for the deuterated and protio bacterial cellulose samples. The highly deuterated bacterial cellulose presented here can be used as a model substrate for studying cellulose biopolymer properties via future small angle neutron scattering (SANS) studies.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Structural Molecular Biology (CSMB)
• Sponsoring Organization: USDOE Office of Science (SC)
• DOE Contract Number: DE-AC05-00OR22725
• OSTI ID: 1078190
• Journal Information: carbohydrate research, Vol. 374; ISSN 0008-6215
• Country of Publication: United States
• Language: English

Related Subjects

Deuterated bacterial cellulose
Acetobacter xylinus
FTIR
Crystallinity