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Title: Reduction of U(VI) and Toxic Metals by Desulfovibrio Cytochrome C3

The central objective of our proposed research was twofold: 1) to investigate the structure-function relationship of Desulfovibrio desulfuricans (now Desulfovibrio alaskensis G20) cytochrome c3 with uranium and 2) to elucidate the mechanism for uranium reduction in vitro and in vivo. Physiological analysis of a mutant of D. desulfuricans with a mutation of the gene encoding the type 1 tetraheme cytochrome c3 had demonstrated that uranium reduction was negatively impacted while sulfate reduction was not if lactate were the electron donor. This was thought to be due to the presence of a branched pathway of electron flow from lactate leading to sulfate reduction. Our experimental plan was to elucidate the structural and mechanistic details of uranium reduction involving cytochrome c3.
Authors:
Publication Date:
OSTI Identifier:
1073608
Report Number(s):
AN 241.3- Final Report
Award Number: COLUM_001383
DOE Contract Number:
FG02-01ER63155
Resource Type:
Technical Report
Research Org:
The Curators of the University of Missouri. Columbia, MO
Sponsoring Org:
USDOE; USDOE SC Office of Biological and Environmental Research (SC-23)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES; 08 HYDROGEN Metal transformations by soil microorganisms Bioremediation of U(VI) Cytochromes of sulfate-reducing bacteria