Engineering Fungal Nonreducing Polyketide Synthase by Heterologous Expression and Domain Swapping
Abstract
Heterologous expression of the A. niger NR-PKS gene, e_gw1_19.204 and the adjacent stand-alone R domain gene, est_GWPlus_C_190476 in A. nidulans demonstrated that they belong to a single gene named dtbA. The DtbA protein produces two polyketides, 2,4-dihydroxy-3,5,6-trimethylbenzaldehyde 1 and 2-ethyl-4,6-dihydroxy-3,5-dimethylbenzaldehyde 2. Generation of DtbA+R-TE chimeric PKSs by swapping the DtbA R domain with the AusA (austinol biosynthesis) or ANID_06448 TE domain enabled the production of two metabolites with carboxylic acids replacing the corresponding aldehydes.
- Authors:
- Publication Date:
- Research Org.:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1067959
- Report Number(s):
- PNNL-SA-92219
Journal ID: ISSN 1523-7060; BM0101020
- DOE Contract Number:
- AC05-76RL01830
- Resource Type:
- Journal Article
- Journal Name:
- Organic Letters
- Additional Journal Information:
- Journal Volume: 15; Journal Issue: 4; Journal ID: ISSN 1523-7060
- Publisher:
- American Chemical Society
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Aspergillus, Polyketide Synthase, Domain Swapping, Thioesterase Domain
Citation Formats
Yeh, Hsu-Hua, Chang, Shu-Lin, Chiang, Yi-Ming, Bruno, Kenneth S., Oakley, Berl R., Wu, Tung-Kung, and Wang, Clay C. C. Engineering Fungal Nonreducing Polyketide Synthase by Heterologous Expression and Domain Swapping. United States: N. p., 2013.
Web. doi:10.1021/ol303328t.
Yeh, Hsu-Hua, Chang, Shu-Lin, Chiang, Yi-Ming, Bruno, Kenneth S., Oakley, Berl R., Wu, Tung-Kung, & Wang, Clay C. C. Engineering Fungal Nonreducing Polyketide Synthase by Heterologous Expression and Domain Swapping. United States. https://doi.org/10.1021/ol303328t
Yeh, Hsu-Hua, Chang, Shu-Lin, Chiang, Yi-Ming, Bruno, Kenneth S., Oakley, Berl R., Wu, Tung-Kung, and Wang, Clay C. C. 2013.
"Engineering Fungal Nonreducing Polyketide Synthase by Heterologous Expression and Domain Swapping". United States. https://doi.org/10.1021/ol303328t.
@article{osti_1067959,
title = {Engineering Fungal Nonreducing Polyketide Synthase by Heterologous Expression and Domain Swapping},
author = {Yeh, Hsu-Hua and Chang, Shu-Lin and Chiang, Yi-Ming and Bruno, Kenneth S. and Oakley, Berl R. and Wu, Tung-Kung and Wang, Clay C. C.},
abstractNote = {Heterologous expression of the A. niger NR-PKS gene, e_gw1_19.204 and the adjacent stand-alone R domain gene, est_GWPlus_C_190476 in A. nidulans demonstrated that they belong to a single gene named dtbA. The DtbA protein produces two polyketides, 2,4-dihydroxy-3,5,6-trimethylbenzaldehyde 1 and 2-ethyl-4,6-dihydroxy-3,5-dimethylbenzaldehyde 2. Generation of DtbA+R-TE chimeric PKSs by swapping the DtbA R domain with the AusA (austinol biosynthesis) or ANID_06448 TE domain enabled the production of two metabolites with carboxylic acids replacing the corresponding aldehydes.},
doi = {10.1021/ol303328t},
url = {https://www.osti.gov/biblio/1067959},
journal = {Organic Letters},
issn = {1523-7060},
number = 4,
volume = 15,
place = {United States},
year = {Fri Feb 15 00:00:00 EST 2013},
month = {Fri Feb 15 00:00:00 EST 2013}
}
Other availability
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.