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Title: Engineering Fungal Nonreducing Polyketide Synthase by Heterologous Expression and Domain Swapping

Abstract

Heterologous expression of the A. niger NR-PKS gene, e_gw1_19.204 and the adjacent stand-alone R domain gene, est_GWPlus_C_190476 in A. nidulans demonstrated that they belong to a single gene named dtbA. The DtbA protein produces two polyketides, 2,4-dihydroxy-3,5,6-trimethylbenzaldehyde 1 and 2-ethyl-4,6-dihydroxy-3,5-dimethylbenzaldehyde 2. Generation of DtbA+R-TE chimeric PKSs by swapping the DtbA R domain with the AusA (austinol biosynthesis) or ANID_06448 TE domain enabled the production of two metabolites with carboxylic acids replacing the corresponding aldehydes.

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1067959
Report Number(s):
PNNL-SA-92219
Journal ID: ISSN 1523-7060; BM0101020
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Organic Letters
Additional Journal Information:
Journal Volume: 15; Journal Issue: 4; Journal ID: ISSN 1523-7060
Publisher:
American Chemical Society
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Aspergillus, Polyketide Synthase, Domain Swapping, Thioesterase Domain

Citation Formats

Yeh, Hsu-Hua, Chang, Shu-Lin, Chiang, Yi-Ming, Bruno, Kenneth S., Oakley, Berl R., Wu, Tung-Kung, and Wang, Clay C. C. Engineering Fungal Nonreducing Polyketide Synthase by Heterologous Expression and Domain Swapping. United States: N. p., 2013. Web. doi:10.1021/ol303328t.
Yeh, Hsu-Hua, Chang, Shu-Lin, Chiang, Yi-Ming, Bruno, Kenneth S., Oakley, Berl R., Wu, Tung-Kung, & Wang, Clay C. C. Engineering Fungal Nonreducing Polyketide Synthase by Heterologous Expression and Domain Swapping. United States. https://doi.org/10.1021/ol303328t
Yeh, Hsu-Hua, Chang, Shu-Lin, Chiang, Yi-Ming, Bruno, Kenneth S., Oakley, Berl R., Wu, Tung-Kung, and Wang, Clay C. C. 2013. "Engineering Fungal Nonreducing Polyketide Synthase by Heterologous Expression and Domain Swapping". United States. https://doi.org/10.1021/ol303328t.
@article{osti_1067959,
title = {Engineering Fungal Nonreducing Polyketide Synthase by Heterologous Expression and Domain Swapping},
author = {Yeh, Hsu-Hua and Chang, Shu-Lin and Chiang, Yi-Ming and Bruno, Kenneth S. and Oakley, Berl R. and Wu, Tung-Kung and Wang, Clay C. C.},
abstractNote = {Heterologous expression of the A. niger NR-PKS gene, e_gw1_19.204 and the adjacent stand-alone R domain gene, est_GWPlus_C_190476 in A. nidulans demonstrated that they belong to a single gene named dtbA. The DtbA protein produces two polyketides, 2,4-dihydroxy-3,5,6-trimethylbenzaldehyde 1 and 2-ethyl-4,6-dihydroxy-3,5-dimethylbenzaldehyde 2. Generation of DtbA+R-TE chimeric PKSs by swapping the DtbA R domain with the AusA (austinol biosynthesis) or ANID_06448 TE domain enabled the production of two metabolites with carboxylic acids replacing the corresponding aldehydes.},
doi = {10.1021/ol303328t},
url = {https://www.osti.gov/biblio/1067959}, journal = {Organic Letters},
issn = {1523-7060},
number = 4,
volume = 15,
place = {United States},
year = {Fri Feb 15 00:00:00 EST 2013},
month = {Fri Feb 15 00:00:00 EST 2013}
}