Time-resolved Hyperspectral Fluorescence Spectroscopy using Frequency Modulated Excitation
Abstract
An intensity-modulated excitation light source is used together with a micro channel plate intensified CCD (ICCD) detector gated at a slightly different frequency to generate a beat frequency from a fluorescent sample. The addition of a spectrograph produces a hyperspectral time-resolved data product where the resulting beat frequency is detected with a low frame rate camera. Measuring the beat frequency of the spectrum as a function of time allows separation of the excited fluorescence from ambient constant light sources. The excitation and detector repetition rates are varied over a range of discrete frequencies, and the phase shift of the beat wave maps out the emission decay rate(s).
- Authors:
- Publication Date:
- Research Org.:
- Nevada Test Site (NTS), Mercury, NV (United States)
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA)
- OSTI Identifier:
- 1055938
- Report Number(s):
- DOE/NV/25946-1462
- DOE Contract Number:
- DE-AC52-06NA25946
- Resource Type:
- Journal Article
- Journal Name:
- Journal of Applied Physics
- Additional Journal Information:
- Journal Volume: 112; Journal Issue: 1
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; Laser-induced fluorescence, time-resolved spectroscopy, frequency modulated spectroscopy, frequency domain, beat frequency, remote sensing, spectroscopy, lifetime, hyperspectral measurements
Citation Formats
Neill, M. Time-resolved Hyperspectral Fluorescence Spectroscopy using Frequency Modulated Excitation. United States: N. p., 2012.
Web. doi:10.1063/1.4731247.
Neill, M. Time-resolved Hyperspectral Fluorescence Spectroscopy using Frequency Modulated Excitation. United States. https://doi.org/10.1063/1.4731247
Neill, M. 2012.
"Time-resolved Hyperspectral Fluorescence Spectroscopy using Frequency Modulated Excitation". United States. https://doi.org/10.1063/1.4731247.
@article{osti_1055938,
title = {Time-resolved Hyperspectral Fluorescence Spectroscopy using Frequency Modulated Excitation},
author = {Neill, M},
abstractNote = {An intensity-modulated excitation light source is used together with a micro channel plate intensified CCD (ICCD) detector gated at a slightly different frequency to generate a beat frequency from a fluorescent sample. The addition of a spectrograph produces a hyperspectral time-resolved data product where the resulting beat frequency is detected with a low frame rate camera. Measuring the beat frequency of the spectrum as a function of time allows separation of the excited fluorescence from ambient constant light sources. The excitation and detector repetition rates are varied over a range of discrete frequencies, and the phase shift of the beat wave maps out the emission decay rate(s).},
doi = {10.1063/1.4731247},
url = {https://www.osti.gov/biblio/1055938},
journal = {Journal of Applied Physics},
number = 1,
volume = 112,
place = {United States},
year = {Sun Jul 01 00:00:00 EDT 2012},
month = {Sun Jul 01 00:00:00 EDT 2012}
}
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