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Title: Interim report on updated microarray probes for the LLNL Burkholderia pseudomallei SNP array

The overall goal of this project is to forensically characterize 100 unknown Burkholderia isolates in the US-Australia collaboration. We will identify genome-wide single nucleotide polymorphisms (SNPs) from B. pseudomallei and near neighbor species including B. mallei, B. thailandensis and B. oklahomensis. We will design microarray probes to detect these SNP markers and analyze 100 Burkholderia genomic DNAs extracted from environmental, clinical and near neighbor isolates from Australian collaborators on the Burkholderia SNP microarray. We will analyze the microarray genotyping results to characterize the genetic diversity of these new isolates and triage the samples for whole genome sequencing. In this interim report, we described the SNP analysis and the microarray probe design for the Burkholderia SNP microarray.
Authors:
;
Publication Date:
OSTI Identifier:
1047245
Report Number(s):
LLNL-TR-543366
TRN: US201216%%374
DOE Contract Number:
W-7405-ENG-48
Resource Type:
Technical Report
Research Org:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; DESIGN; GENETICS; LAWRENCE LIVERMORE NATIONAL LABORATORY; NUCLEOTIDES; PROBES