Complete genome sequence of Rhizobium leguminosarum bv. trifolii strain WSM1325, an effective microsymbiont of annual Mediterranean clovers.

Journal Article · Fri Jan 01 00:00:00 EST 2010 · Standards in Genomic Sciences

DOI:https://doi.org/10.4056/sigs.852027· OSTI ID:1043958

Reeve, Wayne ^[1]; O'Hara, Graham ^[1]; Chain, Patrick S. G. ^[2]; Ardley, Julie ^[1]; Brau, Lambert ^[1]; Nandesena, Kemanthi ^[1]; Tiwari, Ravi ^[1]; Copeland, A ^[3]; Nolan, Matt ^[3]; Han, Cliff ^[4]; Brettin, Thomas S ^[5]; Land, Miriam L ^[5]; Ovchinnikova, Galina ^[3]; Ivanova, N ^[3]; Mavromatis, K ^[3]; Markowitz, Victor ^[3]; Kyrpides, Nikos C ^[3]; Melino, Vanessa ^[1]; Denton, Matthew ^[6]; Yates, Ron ^[1] ; Howieson, John ^[1]

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1. Murdoch University, Perth, Australia
2. Lawrence Livermore National Laboratory (LLNL)
3. U.S. Department of Energy, Joint Genome Institute
4. Los Alamos National Laboratory (LANL)
5. ORNL
6. Department of Primary Industries, Victoria, Australia

Rhizobium leguminosarum bv trifolii is a soil-inhabiting bacterium that that has the capacity to be an effective nitrogen fixing microsymbiont of a diverse range of annual Trifolium (clover) species. Strain WSM1325 is an aerobic, motile, non-spore forming, Gram-negative rod isolated from root nodules collected in 1993 from the Greek Island of Serifos. WSM1325 is manufactured commercially in Australia as an inoculant for a broad range of annual clovers of Mediterranean origin due to its superior attributes of saprophytic competence, nitrogen fixation and acid-tolerance. Here we describe the basic features of this organism, together with the complete genome sequence, and annotation. This is the first completed genome sequence for a microsymbiont of annual clovers. We reveal that its genome size is 7,418,122 bp encoding 7,232 protein-coding genes and 61 RNA-only encoding genes. This multipartite genome contains 6 distinct replicons; a chromosome of size 4,767,043 bp and 5 plasmids of size 828,924, 660,973, 516,088, 350,312 and 294,782 bp.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: Work for Others (WFO)
• DOE Contract Number: DE-AC05-00OR22725
• OSTI ID: 1043958
• Journal Information: Standards in Genomic Sciences, Vol. 2, Issue 3; ISSN 1944-3277
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
CAPACITY
CHROMOSOMES
CLOVER
GENES
NITROGEN
NITROGEN FIXATION
ORIGIN
PLASMIDS
REPLICONS
RHIZOBIUM
STRAINS