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Title: Replication of N[superscript 2],3-Ethenoguanine by DNA Polymerases

The unstable DNA adduct N2,3-ethenoguanine, a product of both exposure to the carcinogen vinyl chloride and of oxidative stress, was built into an oligonucleotide, using an isostere strategy to stabilize the glycosidic bond. This modification was then used to examine the cause of mutations by DNA polymerases, in terms of both the biochemistry of the lesion and a structure of the lesion within a polymerase.
Authors:
; ; ; ; ; ; ;  [1]
  1. (Vanderbilt)
Publication Date:
OSTI Identifier:
1043730
Resource Type:
Journal Article
Resource Relation:
Journal Name: Angewandte Chemie (International Edition); Journal Volume: 51; Journal Issue: (22) ; 05, 2012
Research Org:
Advanced Photon Source (APS), Argonne National Laboratory (ANL), Argonne, IL (US)
Sponsoring Org:
OTHER U.S. GOVERNMENT
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; BIOCHEMISTRY; CARCINOGENS; DNA; DNA ADDUCTS; DNA DAMAGES; DNA POLYMERASES; DNA REPLICATION; MODIFICATIONS; MUTAGENESIS; MUTATIONS; VINYL CHLORIDE