Laser Microperforated Biodegradable Microbial Polyhydroxyalkanoate Substrates for Tissue Repair Strategies: An Infrared Microspectroscopy Studey
Flexible and biodegradable film substrates prepared by solvent casting from poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBHV) solutions in chloroform were microperforated by ultraviolet laser ablation and subsequently characterized using infrared (IR) microspectroscopy and imaging techniques and scanning electron microscopy (SEM). Both transmission synchrotron IR microspectroscopy and attenuated total reflectance microspectroscopy measurements demonstrate variations in the polymer at the ablated pore rims, including evidence for changes in chemical structure and crystallinity. SEM results on microperforated PHBHV substrates after cell culture demonstrated that the physical and chemical changes observed in the biomaterial did not hinder cell migration through the pores.
- Research Organization:
- Brookhaven National Lab. (BNL), Upton, NY (United States)
- Sponsoring Organization:
- USDOE SC OFFICE OF SCIENCE (SC)
- DOE Contract Number:
- DE-AC02-98CH10886
- OSTI ID:
- 1041839
- Report Number(s):
- BNL-97517-2012-JA; TRN: US201212%%251
- Journal Information:
- Analytical and Bioanalytical Chemistry, Vol. 399, Issue 7; ISSN 1618-2642
- Country of Publication:
- United States
- Language:
- English
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