Implementations of two-dimensional liquid chromatography

Guiochon, Georges A; Marchetti, Nicola; Mriziq, Khaled S; Shalliker, R. Andrew

doi:10.1016/j.chroma.2008.01.086

Title: Implementations of two-dimensional liquid chromatography

Full Record
Other Related Research

Abstract

Today scientists must deal with complex samples that either cannot be adequately separated using one-dimensional chromatography or that require an inordinate amount of time for separation. For these cases we need two-dimensional chromatography because it takes far less time to generate a peak capacity n{sub c} twice in a row than to generate a peak capacity n{sub c}{sup 2} once. Liquid chromatography has been carried out successfully on thin layers of adsorbents and along tubes filled with various adsorbents. The first type of separation sorts out the sample components in a physical separation space that is the layer of packing material. The analysis time is the same for all the components of the sample while their migration distance increases with decreasing retention. The resolution between two components having a certain separation factor (a) increases with increasing migration distance, i.e., from the strongly to the weakly retained compounds. In the second type of separation, the sample components are eluted from the column and separated in the time space, their migration distances are all the same while their retention times increase from the unretained to the strongly retained compounds. Separation efficiency varies little with retention, as long as the components are elutedmore »« less

Authors:

Guiochon, Georges A ^[1]; Marchetti, Nicola ^[2]; Mriziq, Khaled S ^[1]; Shalliker, R. Andrew ^[3]

ORNL
University of Tennessee, Knoxville (UTK)
University of Western Sydney, Australia

Publication Date:: Tue Jan 01 00:00:00 EST 2008

Research Org.:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1041453

DOE Contract Number:: DE-AC05-00OR22725

Resource Type:: Journal Article

Journal Name:: Journal of Chromatography A

Additional Journal Information:: Journal Volume: 1189; Journal Issue: 1-2; Journal ID: ISSN 0021-9673

Country of Publication:: United States

Language:: English

Subject:: 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; ADSORBENTS; CAPACITY; CHROMATOGRAPHY; EFFICIENCY; PERFORMANCE; RESOLUTION; RETENTION; THIN-LAYER CHROMATOGRAPHY

Citation Formats


                    Guiochon, Georges A, Marchetti, Nicola, Mriziq, Khaled S, and Shalliker, R. Andrew. Implementations of two-dimensional liquid chromatography.  United States: N. p., 2008. 
        Web.  doi:10.1016/j.chroma.2008.01.086.

Copy to clipboard


                    Guiochon, Georges A, Marchetti, Nicola, Mriziq, Khaled S, & Shalliker, R. Andrew. Implementations of two-dimensional liquid chromatography.  United States.  https://doi.org/10.1016/j.chroma.2008.01.086

Copy to clipboard


                    Guiochon, Georges A, Marchetti, Nicola, Mriziq, Khaled S, and Shalliker, R. Andrew. 2008.  
        "Implementations of two-dimensional liquid chromatography".  United States.  https://doi.org/10.1016/j.chroma.2008.01.086.

Copy to clipboard


                    
@article{osti_1041453,

  title        = {Implementations of two-dimensional liquid chromatography},

  author       = {Guiochon, Georges A and Marchetti, Nicola and Mriziq, Khaled S and Shalliker, R. Andrew},

  abstractNote = {Today scientists must deal with complex samples that either cannot be adequately separated using one-dimensional chromatography or that require an inordinate amount of time for separation. For these cases we need two-dimensional chromatography because it takes far less time to generate a peak capacity n{sub c} twice in a row than to generate a peak capacity n{sub c}{sup 2} once. Liquid chromatography has been carried out successfully on thin layers of adsorbents and along tubes filled with various adsorbents. The first type of separation sorts out the sample components in a physical separation space that is the layer of packing material. The analysis time is the same for all the components of the sample while their migration distance increases with decreasing retention. The resolution between two components having a certain separation factor (a) increases with increasing migration distance, i.e., from the strongly to the weakly retained compounds. In the second type of separation, the sample components are eluted from the column and separated in the time space, their migration distances are all the same while their retention times increase from the unretained to the strongly retained compounds. Separation efficiency varies little with retention, as long as the components are eluted from the column. We call these two types of separation the chromatographic separations in space (LC{sup x}) and the chromatographic separations in time (LC{sup t}), respectively. In principle, there are four ways to combine these two modes and do two-dimensional chromatographic separations, LC{sup t} x LC{sup t}, LC{sup x} x LC{sup t}, LC{sup t} x LC{sup x}, and LC{sup x} x LC{sup x}. We review, discuss and compare the potential performance of these combinations, their advantages, drawbacks, problems, perspectives and results. Currently, column-based combinations (LC{sup t} x LC{sup t}) are the most actively pursued. We suggest that the combination LC{sup x} x LC{sup t} shows exceptional promise because it permits the simultaneous second-dimension separations of all the fractions separated in the first-dimension, thus providing remarkable time saving.},

  doi          = {10.1016/j.chroma.2008.01.086},

  url          = {https://www.osti.gov/biblio/1041453},
  journal      = {Journal of Chromatography A},

  issn         = {0021-9673},

  number       = 1-2,

  volume       = 1189,

  place        = {United States},

  year         = {Tue Jan 01 00:00:00 EST 2008},

  month        = {Tue Jan 01 00:00:00 EST 2008}

}

Copy to clipboard

Journal Article:

https://doi.org/10.1016/j.chroma.2008.01.086

Other availability

Find in Google Scholar

Search WorldCat to find libraries that may hold this journal

Save / Share:

Export Metadata

Save to My Library

Similar records in OSTI.GOV collections:

Column properties and flow profiles of a flat, wide column for high-pressure liquid chromatography

Journal Article Mriziq, Khaled; Guiochon, Georges - Journal of Chromatography A

The design and the construction of a pressurized, flat, wide column for high-performance liquid chromatography (HPLC) are described. This apparatus, which is derived from instruments that implement over-pressured thin layer chromatography, can carry out only uni-dimensional chromatographic separations. However, it is intended to be the first step in the development of more powerful instruments that will be able to carry out two-dimensional chromatographic separations, in which case, the first separation would be a space-based separation, LC{sup x}, taking place along one side of the bed and the second separation would be a time-based separation, LC{sup t}, as in classical HPLCmore »« less
https://doi.org/10.1016/j.chroma.2008.02.037
Numerical modeling of elution peak profiles in supercritical fluid chromatography. Part I-Elution of an unretained tracer

Journal Article Kaczmarski, Krzysztof; Guiochon, Georges - Journal of Chromatography A

When chromatography is carried out with high-density carbon dioxide as the main component of the mobile phase (a method generally known as 'supercritical fluid chromatography' or SFC), the required pressure gradient along the column is moderate. However, this mobile phase is highly compressible and, under certain experimental conditions, its density may decrease significantly along the column. Such an expansion absorbs heat, cooling the column, which absorbs heat from the outside. The resulting heat transfer causes the formation of axial and radial gradients of temperature that may become large under certain conditions. Due to these gradients, the mobile phase velocity andmore »« less
https://doi.org/10.1016/j.chroma.2010.08.035
Separation of nonvolatile components of fossil fuels at very high resolution and their structural characterization. Progress report, May 1, 1985-January 31, 1986

Technical Report Novotny, M

The recently developed miniature photodiode array fluorescence detector has now been applied in conjunction with microcolumn liquid chromatography to several classes of compounds. While our typical micro-LC separations (efficiencies in the excess of 250,000 theoretical plates) yield between 50 and 100 resolved peaks, numerous spectra are taken on-line during these chromatograms. A very efficient data acquisition and processing system was developed whereby approximately, 375 spectra can be recorded on a single floppy disk. Numerous fossil fuel and related samples have now been processed through this combination: neutral polyaromatics from carbon black; nitrogen polycyclics; sulfur-containing polycyclics; and phenolic. The greatest problemmore »« less
Simultaneous concentration and purification through gradient deformation chromatography

Journal Article Velayudhan, A; Hendrickson, R; Ladisch, M - AIChE Journal

Mobile-phase additives, commonly used to modulate absorbate retention in gradient elution chromatography, are usually assumed to be either linearly retained or unretained. Previous theoretical work from the laboratory has shown that these modulators, such as salts in ion-exchange and hydrophobic interaction chromatography and organic modifiers in reversed-phase chromatography, can absorb nonlinearly, giving rise to gradient deformation. Consequently, adsorbate peaks that elute in the vicinity of the head of the deformed gradient may exhibit unusual shapes, form shoulders, and/or be concentrated. These effects for a reversed-phase sorbent with aqueous acetonitrile (ACN) as the modulator are verified experimentally. Gradient deformation is demonstratedmore »« less
https://doi.org/10.1002/aic.690410514
Development and optimization of methodologies for analysis fo complex hydrocarbon mixtures. Technical progress report, 1 September 1980-31 August 1981

Technical Report Laub, R

Studies of rotating-disk thin-layer chromatography were continued from the previous grant period, and fundamental aspects of this newly-revived technique were examined in this period in some detail. A number of design innovations were explored, implementation of which could result in a separations system which rivals conventional-column lc for pre-analysis fractionation of petroleum. Studies with liquid-chromatographic (lc) columns of conventional dimensions examined the variation of retentions of aromatic hydrocarbon solutes with mobile phases of methanol + water (reversed-phase sorbent) and hexane + isopropyl alcohol (silica sorbent). The utility of ternary blends of hexane solvent + combinations of methylene chloride, tetrahydrofuran, acetonitrile,more »« less

Similar Records