Linking specific heterotrophic bacterial populations to bioreduction of uranium and nitrate using stable isotope probing in contaminated subsurface sediments

Journal Article · Sat Jan 01 00:00:00 EST 2011 · Applied and Environmental Microbiology

DOI:https://doi.org/10.1128/AEM.05247-11· OSTI ID:1037029

Akob, Denise M ^[1]; Kerkhof, Lee ^[2]; Kusel, Kirsten ^[3]; Watson, David B ^[4]; Palumbo, Anthony Vito ^[4]; Kostka, Joel ^[1]

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1. Florida State University
2. Rutgers University
3. Friedrich Schiller University Jena, Jena Germany
4. ORNL

Shifts in terminal electron-accepting processes during biostimulation of uranium-contaminated sediments were linked to the composition of stimulated microbial populations using DNA-based stable isotope probing. Nitrate reduction preceded U(VI) and Fe(III) reduction in [{sup 13}C]ethanol-amended microcosms. The predominant, active denitrifying microbial groups were identified as members of the Betaproteobacteria, whereas Actinobacteria dominated under metal-reducing conditions.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: USDOE Office of Science (SC)
• DOE Contract Number: DE-AC05-00OR22725
• OSTI ID: 1037029
• Journal Information: Applied and Environmental Microbiology, Vol. 77, Issue 22; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

12 MANAGEMENT OF RADIOACTIVE WASTES, AND NON-RADIOACTIVE WASTES FROM NUCLEAR FACILITIES
MICROCOSMS
NITRATES
SEDIMENTS
STABLE ISOTOPES
URANIUM