Application of phenotypic microarrays to environmental microbiology

Journal Article · Sun Jan 01 00:00:00 EST 2012 · Current Opinion in Biotechnology

DOI:https://doi.org/10.1016/j.copbio.2011.12.006· OSTI ID:1034387

Borglin, sharon ^[1]; Joyner, Dominique C. ^[1]; DeAngelis, Kristen ^[1]; Khudyakov, Jane ^[2]; D'haeseleer, Patrik ^[2]; Joachimiak, Marcin ^[1]; Hazen, Terry C.  ^[3]; Fagan, Lisa Anne ^[3]

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1. Lawrence Berkeley National Laboratory (LBNL)
2. Joint Bioenergy Institute
3. ORNL

Environmental organisms are extremely diverse and only a small fraction has been successfully cultured in the laboratory. Culture in micro wells provides a method for rapid screening of a wide variety of growth conditions and commercially available plates contain a large number of substrates, nutrient sources, and inhibitors, which can provide an assessment of the phenotype of an organism. This review describes applications of phenotype arrays to anaerobic and thermophilic microorganisms, use of the plates in stress response studies, in development of culture media for newly discovered strains, and for assessment of phenotype of environmental communities. Also discussed are considerations and challenges in data interpretation and visualization, including data normalization, statistics, and curve fitting.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-00OR22725
• OSTI ID: 1034387
• Journal Information: Current Opinion in Biotechnology, Vol. 23, Issue 1; ISSN 0958-1669
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
54 ENVIRONMENTAL SCIENCES
ANAEROBIC CONDITIONS
BIOLOGICAL STRESS
CULTURE MEDIA
MICROORGANISMS
NUTRIENTS
PHENOTYPE
PLATES
SUBSTRATES
THERMOPHILIC CONDITIONS