skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Molecular Packing of Functionalized Fluorinated Lipids in Langmuir Monolayers

Abstract

Fluorinated amphipaths are a fascinating class of compounds, which, despite significant challenges associated with their syntheses, have found use across a number of areas of biotechnology. Applications range from the in vitro stabilization of membrane proteins to the development of enhanced stability intravenous drug and gene delivery systems. More recently, monolayer-forming fluorinated lipids have found use in the 2D crystallization of detergent-solubilized hydrophobic or partially hydrophobic proteins at the air-water interface. In this study, we investigate the surface properties of a novel suite of monolayer forming, partially fluorinated lipids. These modular lipid structures contain a densely fluorinated insertion in the hydrocarbon tail and a synthetically modifiable headgroup. Analyses of surface-pressure area isotherms and X-ray reflectometry profiles reveal that the lipids spread into fluid monolayers and are more compressible than their non-fluorinated counterparts. Furthermore, the data support a model whereby the partially fluorinated chains of the lipid tails form a film which is fundamentally incompatible with detergents and other destabilizing amphipaths.

Authors:
; ; ; ; ;  [1]
  1. Queens
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
FOREIGN
OSTI Identifier:
1029001
Resource Type:
Journal Article
Journal Name:
Langmuir
Additional Journal Information:
Journal Volume: 26; Journal Issue: (24) ; 12, 2010; Journal ID: ISSN 0743-7463
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; BIOTECHNOLOGY; CRYSTALLIZATION; DETERGENTS; GENES; HYDROCARBONS; IN VITRO; ISOTHERMS; LIPIDS; MEMBRANE PROTEINS; PROTEINS; STABILITY; STABILIZATION; SURFACE PROPERTIES

Citation Formats

Landsberg, Michael J, Ruggles, Jeremy L, Hussein, Waleed M, McGeary, Ross P, Gentle, Ian R, and Hankamer, Ben. Molecular Packing of Functionalized Fluorinated Lipids in Langmuir Monolayers. United States: N. p., 2012. Web. doi:10.1021/la103743e.
Landsberg, Michael J, Ruggles, Jeremy L, Hussein, Waleed M, McGeary, Ross P, Gentle, Ian R, & Hankamer, Ben. Molecular Packing of Functionalized Fluorinated Lipids in Langmuir Monolayers. United States. https://doi.org/10.1021/la103743e
Landsberg, Michael J, Ruggles, Jeremy L, Hussein, Waleed M, McGeary, Ross P, Gentle, Ian R, and Hankamer, Ben. 2012. "Molecular Packing of Functionalized Fluorinated Lipids in Langmuir Monolayers". United States. https://doi.org/10.1021/la103743e.
@article{osti_1029001,
title = {Molecular Packing of Functionalized Fluorinated Lipids in Langmuir Monolayers},
author = {Landsberg, Michael J and Ruggles, Jeremy L and Hussein, Waleed M and McGeary, Ross P and Gentle, Ian R and Hankamer, Ben},
abstractNote = {Fluorinated amphipaths are a fascinating class of compounds, which, despite significant challenges associated with their syntheses, have found use across a number of areas of biotechnology. Applications range from the in vitro stabilization of membrane proteins to the development of enhanced stability intravenous drug and gene delivery systems. More recently, monolayer-forming fluorinated lipids have found use in the 2D crystallization of detergent-solubilized hydrophobic or partially hydrophobic proteins at the air-water interface. In this study, we investigate the surface properties of a novel suite of monolayer forming, partially fluorinated lipids. These modular lipid structures contain a densely fluorinated insertion in the hydrocarbon tail and a synthetically modifiable headgroup. Analyses of surface-pressure area isotherms and X-ray reflectometry profiles reveal that the lipids spread into fluid monolayers and are more compressible than their non-fluorinated counterparts. Furthermore, the data support a model whereby the partially fluorinated chains of the lipid tails form a film which is fundamentally incompatible with detergents and other destabilizing amphipaths.},
doi = {10.1021/la103743e},
url = {https://www.osti.gov/biblio/1029001}, journal = {Langmuir},
issn = {0743-7463},
number = (24) ; 12, 2010,
volume = 26,
place = {United States},
year = {Fri Jan 20 00:00:00 EST 2012},
month = {Fri Jan 20 00:00:00 EST 2012}
}