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Title: A heterodimeric complex of the LRR proteins LRIM1 and APL1C regulates complement-like immunity in Anopheles gambiae

Abstract

The leucine-rich repeat (LRR) proteins LRIM1 and APL1C control the function of the complement-like protein TEP1 in Anopheles mosquitoes. The molecular structure of LRIM1 and APL1C and the basis of their interaction with TEP1 represent a new type of innate immune complex. The LRIM1/APL1C complex specifically binds and solubilizes a cleaved form of TEP1 without an intact thioester bond. The LRIM1 and APL1C LRR domains have a large radius of curvature, glycosylated concave face, and a novel C-terminal capping motif. The LRIM1/APL1C complex is a heterodimer with a single intermolecular disulfide bond. The structure of the LRIM1/APL1C heterodimer reveals an interface between the two LRR domains and an extensive C-terminal coiled-coil domain. We propose that a cleaved form of TEP1 may act as a convertase for activation of other TEP1 molecules and that the LRIM1/APL1C heterodimer regulates formation of this TEP1 convertase.

Authors:
; ; ; ; ;  [1]
  1. CNRS-UMR
Publication Date:
Research Org.:
Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
FOREIGNOTHERHHMI
OSTI Identifier:
1027151
Resource Type:
Journal Article
Journal Name:
Proc. Natl. Acad. Sci. USA
Additional Journal Information:
Journal Volume: 107; Journal Issue: (39) ; 09, 2010; Journal ID: ISSN 0027-8424
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; DISULFIDES; IMMUNITY; MALARIA; MOLECULAR STRUCTURE; MOSQUITOES; PROTEINS

Citation Formats

Baxter, Richard H.G., Steinert, Stefanie, Chelliah, Yogarany, Volohonsky, Gloria, Levashina, Elena A, Deisenhofer, Johann, and UTSMC). A heterodimeric complex of the LRR proteins LRIM1 and APL1C regulates complement-like immunity in Anopheles gambiae. United States: N. p., 2012. Web. doi:10.1073/pnas.1010575107.
Baxter, Richard H.G., Steinert, Stefanie, Chelliah, Yogarany, Volohonsky, Gloria, Levashina, Elena A, Deisenhofer, Johann, & UTSMC). A heterodimeric complex of the LRR proteins LRIM1 and APL1C regulates complement-like immunity in Anopheles gambiae. United States. https://doi.org/10.1073/pnas.1010575107
Baxter, Richard H.G., Steinert, Stefanie, Chelliah, Yogarany, Volohonsky, Gloria, Levashina, Elena A, Deisenhofer, Johann, and UTSMC). 2012. "A heterodimeric complex of the LRR proteins LRIM1 and APL1C regulates complement-like immunity in Anopheles gambiae". United States. https://doi.org/10.1073/pnas.1010575107.
@article{osti_1027151,
title = {A heterodimeric complex of the LRR proteins LRIM1 and APL1C regulates complement-like immunity in Anopheles gambiae},
author = {Baxter, Richard H.G. and Steinert, Stefanie and Chelliah, Yogarany and Volohonsky, Gloria and Levashina, Elena A and Deisenhofer, Johann and UTSMC)},
abstractNote = {The leucine-rich repeat (LRR) proteins LRIM1 and APL1C control the function of the complement-like protein TEP1 in Anopheles mosquitoes. The molecular structure of LRIM1 and APL1C and the basis of their interaction with TEP1 represent a new type of innate immune complex. The LRIM1/APL1C complex specifically binds and solubilizes a cleaved form of TEP1 without an intact thioester bond. The LRIM1 and APL1C LRR domains have a large radius of curvature, glycosylated concave face, and a novel C-terminal capping motif. The LRIM1/APL1C complex is a heterodimer with a single intermolecular disulfide bond. The structure of the LRIM1/APL1C heterodimer reveals an interface between the two LRR domains and an extensive C-terminal coiled-coil domain. We propose that a cleaved form of TEP1 may act as a convertase for activation of other TEP1 molecules and that the LRIM1/APL1C heterodimer regulates formation of this TEP1 convertase.},
doi = {10.1073/pnas.1010575107},
url = {https://www.osti.gov/biblio/1027151}, journal = {Proc. Natl. Acad. Sci. USA},
issn = {0027-8424},
number = (39) ; 09, 2010,
volume = 107,
place = {United States},
year = {Fri Jan 20 00:00:00 EST 2012},
month = {Fri Jan 20 00:00:00 EST 2012}
}