Dispersion stabilization of silver nanoparticles in synthetic lung fluid studied under in situ conditions
- NIST
The dispersion stabilization of silver nanoparticles (AgNPs) in synthetic lung fluid was studied to interrogate the effects on colloidal stability due to the principal constituents of the fluid. The colloidal stability of 20 nm citrate-AgNPs dispersed in the presence of each constituent of the synthetic lung fluid (individually, the complete fluid, and without additives) was observed during titration of increasing sodium chloride concentration. A variety of complementary in situ measurement techniques were utilized, including dynamic light scattering, ultraviolet-visible absorption spectroscopy, atomic force microscopy, and small-angle X-ray scattering, which provided a collective set of information that enabled far better understanding of the dispersion behavior in the fluid than any one technique alone. It was observed that AgNPs continued to adsorb bovine serum albumin (BSA) protein from the synthetic lung fluid solution as the sodium chloride concentration increased, until a maximum BSA coating was achieved prior to reaching the physiological sodium chloride concentration of 154 mmol L{sup -1}. BSA was determined to be the constituent of the synthetic lung fluid that is required to provide colloidal stability at high salt loadings, though the phospholipid constituent exerts a subtle effect. Additionally, as AgNPs are a distinctly different class of nanoparticles apart from the carbon nanotubes and titanium dioxide nanoparticles initially reported to be dispersible using this fluid, this work also demonstrates the broad applicability of synthetic lung fluid in providing stable dispersions for engineered nanoparticles for use in biological assays.
- Research Organization:
- Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
- Sponsoring Organization:
- NSFDOE - BASIC ENERGY SCIENCES
- OSTI ID:
- 1025617
- Journal Information:
- Nanotoxicology, Vol. 5, Issue 06, 2011
- Country of Publication:
- United States
- Language:
- ENGLISH
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Related Subjects
60 APPLIED LIFE SCIENCES
77 NANOSCIENCE AND NANOTECHNOLOGY
ABSORPTION SPECTROSCOPY
ADDITIVES
ALBUMINS
ATOMIC FORCE MICROSCOPY
CARBON
CATTLE
COATINGS
LIGHT SCATTERING
LUNGS
NANOTUBES
PHOSPHOLIPIDS
PROTEINS
SCATTERING
SILVER
SODIUM CHLORIDES
STABILITY
STABILIZATION
TITANIUM
TITRATION