A phosphate-dependent shift in redox state of cerium oxide nanoparticles and its effects on catalytic properties

Journal Article · Sat Oct 01 00:00:00 EDT 2011 · Biomaterials, 32(28):6745-6753

DOI:https://doi.org/10.1016/j.biomaterials.2011.05.073· OSTI ID:1025084

Singh, Sanjay; Dosani, Talib; Karakoti, Ajay S; Kumar, Amit; Seal, Sudipta; Self, William

Cerium oxide nanoparticles (CeNPs) have shown promise as catalytic antioxidants in cell culture and animal models as both superoxide dismutase and catalase mimetics. The reactivity of the cerium (Ce) atoms at the surface of its oxide particle is critical to such therapeutic properties, yet little is known about the potential for a protein or small molecule corona to form on these materials in vivo. Moreover Ce atoms in these active sites have the potential to interact with small molecule anions, peptides, or sugars when administered in culture or animal models. Several nanomaterials have been shown to alter or aggregate under these conditions, rendering them less useful for biomedical applications. In this work we have studied the change in catalytic properties of CeNPs when exposed to various biologically relevant conditions in vitro. We have found that CeNPs are resistant to broad changes in pH and also not altered by incubation in cell culture medium. However to our surprise phosphate anions significantly altered the characteristics of these nanomaterials and shifted the catalytic behavior due to the binding of phosphate anions to cerium. Given the abundance of phosphate in biological systems in an inorganic form, it is likely that the action of CeNPs as a catalyst may be strongly influenced by the local concentration of phosphate in the cells and/or tissues in which it has been introduced.

• Research Organization: Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 1025084
• Report Number(s): PNNL-SA-81172; 30507; KP1704020; TRN: US201120%%268
• Journal Information: Biomaterials, 32(28):6745-6753, Vol. 32, Issue 28
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
ABUNDANCE
ANIMALS
ANIONS
ANTIOXIDANTS
ATOMS
CATALASE
CATALYSTS
CELL CULTURES
CERIUM
CERIUM OXIDES
IN VITRO
IN VIVO
INCUBATION
OXIDES
PEPTIDES
PHOSPHATES
PROTEINS
SACCHARIDES
SUPEROXIDE DISMUTASE
Phosphate
dependent
shift
redox
state
cerium
oxide
nanoparticles
Environmental Molecular Sciences Laboratory