skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Chromium(VI) bioremoval by pseudomonas bacteria: role of microbial exudates for natural attenuation and biotreatment of Cr(VI) contamination

Abstract

Laboratory batch and column experiments were conducted to investigate the role of microbial exudates, e.g., exopolymeric substance (EPS) and alginic acid, on microbial Cr(VI) reduction by two different Pseudomonas strains (P. putida P18 and P. aeuroginosa P16) as a method for treating subsurface environment contaminated with Cr(VI). Our results indicate that microbial exudates significantly enhanced microbial Cr(VI) reduction rates by forming less toxic and highly soluble organo-Cr(III) complexes despite the fact Cr(III) has a very low solubility under the experimental conditions studied (e.g., pH 7). The formation of soluble organo-Cr(III) complexes led to the protection of the cells and chromate reductases from inactivation. In systems with no organic ligands, soluble organo-Cr(III) end products were formed between Cr(III) and the EPS directly released by bacteria due to cell lysis. Our results also provide evidence that cell lysis played an important role in microbial Cr(VI) reduction by Pseudomonas bacteria due to the release of constitutive reductases that intracellularly and/or extracellularly catalyzed the reduction of Cr(VI) to Cr(III). The overall results highlight the need for incorporation of the release and formation of organo-Cr(III) complexes into reactive transport models to more accurately design and monitor in situ microbial remediation techniques for the treatment ofmore » subsurface systems contaminated with Cr(VI).« less

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
DOE - OFFICE OF SCIENCE
OSTI Identifier:
1019480
Report Number(s):
BNL-94981-2011-JA
Journal ID: ISSN 0013-936X; ESTHAG; R&D Project: KBCH139; 00600; KB0202011; TRN: US201115%%154
DOE Contract Number:  
DE-AC02-98CH10886
Resource Type:
Journal Article
Journal Name:
Environmental Science and Technology
Additional Journal Information:
Journal Volume: 45; Journal Issue: 6; Journal ID: ISSN 0013-936X
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; ALGINIC ACID; BACTERIA; CHROMATES; CONTAMINATION; DESIGN; INACTIVATION; LYSIS; MONITORS; NATURAL ATTENUATION; OXIDOREDUCTASES; PSEUDOMONAS; SOLUBILITY; STRAINS; TRANSPORT

Citation Formats

Dogan, N M, Dodge, C, Kantar, C, Gulcan, S, Yilmaz, B C, and Mazmanci, M A. Chromium(VI) bioremoval by pseudomonas bacteria: role of microbial exudates for natural attenuation and biotreatment of Cr(VI) contamination. United States: N. p., 2011. Web. doi:10.1021/es102095t.
Dogan, N M, Dodge, C, Kantar, C, Gulcan, S, Yilmaz, B C, & Mazmanci, M A. Chromium(VI) bioremoval by pseudomonas bacteria: role of microbial exudates for natural attenuation and biotreatment of Cr(VI) contamination. United States. https://doi.org/10.1021/es102095t
Dogan, N M, Dodge, C, Kantar, C, Gulcan, S, Yilmaz, B C, and Mazmanci, M A. 2011. "Chromium(VI) bioremoval by pseudomonas bacteria: role of microbial exudates for natural attenuation and biotreatment of Cr(VI) contamination". United States. https://doi.org/10.1021/es102095t.
@article{osti_1019480,
title = {Chromium(VI) bioremoval by pseudomonas bacteria: role of microbial exudates for natural attenuation and biotreatment of Cr(VI) contamination},
author = {Dogan, N M and Dodge, C and Kantar, C and Gulcan, S and Yilmaz, B C and Mazmanci, M A},
abstractNote = {Laboratory batch and column experiments were conducted to investigate the role of microbial exudates, e.g., exopolymeric substance (EPS) and alginic acid, on microbial Cr(VI) reduction by two different Pseudomonas strains (P. putida P18 and P. aeuroginosa P16) as a method for treating subsurface environment contaminated with Cr(VI). Our results indicate that microbial exudates significantly enhanced microbial Cr(VI) reduction rates by forming less toxic and highly soluble organo-Cr(III) complexes despite the fact Cr(III) has a very low solubility under the experimental conditions studied (e.g., pH 7). The formation of soluble organo-Cr(III) complexes led to the protection of the cells and chromate reductases from inactivation. In systems with no organic ligands, soluble organo-Cr(III) end products were formed between Cr(III) and the EPS directly released by bacteria due to cell lysis. Our results also provide evidence that cell lysis played an important role in microbial Cr(VI) reduction by Pseudomonas bacteria due to the release of constitutive reductases that intracellularly and/or extracellularly catalyzed the reduction of Cr(VI) to Cr(III). The overall results highlight the need for incorporation of the release and formation of organo-Cr(III) complexes into reactive transport models to more accurately design and monitor in situ microbial remediation techniques for the treatment of subsurface systems contaminated with Cr(VI).},
doi = {10.1021/es102095t},
url = {https://www.osti.gov/biblio/1019480}, journal = {Environmental Science and Technology},
issn = {0013-936X},
number = 6,
volume = 45,
place = {United States},
year = {Mon Feb 14 00:00:00 EST 2011},
month = {Mon Feb 14 00:00:00 EST 2011}
}