Membrane-Based Emitter for Coupling Microfluidics with Ultrasensitive Nanoelectrospray Ionization-Mass Spectrometry
An integrated poly(dimethylsiloxane) (PDMS) membrane-based microfluidic emitter for high performance nanoelectrospray ionization-mass spectrometry (nanoESI-MS) has been fabricated and evaluated. The ~100-μm-thick emitter was created by cutting a PDMS membrane that protrudes beyond the bulk substrate. The reduced surface area at the emitter enhances the electric field and reduces wetting of the surface by the electrospray solvent. As such, the emitter provides highly stable electrospray at flow rates as low as 10 nL/min, and is compatible with electrospray solvents containing a large organic component (e.g., 90% methanol). This approach enables facile emitter construction, and provides excellent stability, reproducibility and sensitivity, as well as compatibility with multilayer soft lithography.
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 1019196
- Report Number(s):
- PNNL-SA-79137; 40072; 20492a; 24698; 42691; 400412000; TRN: US201114%%681
- Journal Information:
- Analytical Chemistry, 83(4):5797-5803, Vol. 83, Issue 14
- Country of Publication:
- United States
- Language:
- English
Similar Records
Robust Extraction Interface for Coupling Droplet-Based and Continuous Flow Microfluidics
Multinozzle Emitter Arrays for Nanoelectrospray Mass Spectrometry