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Title: Chemical interactions between protein molecules and polymer membrane materials. Annual progress report, February 1, 1994--October 31, 1994

During the past year, the authors have used the Surface Forces Apparatus (SFA) to measure the intermolecular forces between a model protein (hen egg-white lysozyme) and a model hydrophilic surface (mica), between lysozyme and itself and between lysozyme and a model hydrophobic surface composed of a crosslinked alkoxysilane surfactant (hexadecyltriethoxysilane, HTE). As expected, repulsive forces are dominant between the hydrophilic surfaces with the same charge (lysozyme-lysozyme) while attractive forces are dominant between oppositely charged surfaces (lysozyme-mica) and between the lysozyme and the hydrophobic surface. The DLVO theory for charged surfaces was found to agree with the results of the lysozyme-lysozyme interaction. Efforts also have been focused on trying to create a well-formed, defect-free monolayer of the HTE on the surface of the mica using a Langmuir-Blodgett (LB) apparatus. A smooth, defect-free surface is desired for the intermolecular force studies. Atomic force microscopy has been used to determine the topography of the HTE films.
Authors:
;
Publication Date:
OSTI Identifier:
10190597
Report Number(s):
DOE/ER/14114--4
ON: DE95001808; TRN: 94:009702
DOE Contract Number:
FG02-90ER14114
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: 25 Aug 1994
Research Org:
Rensselaer Polytechnic Inst., Troy, NY (United States)
Sponsoring Org:
USDOE, Washington, DC (United States)
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; PROTEINS; SURFACE FORCES; MEMBRANES; PROGRESS REPORT; CHEMICAL REACTIONS; MATHEMATICAL MODELS; LYSOZYME; MICA; SURFACTANTS 400201; CHEMICAL AND PHYSICOCHEMICAL PROPERTIES