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Title: Chromosomal localization and structure of the human type II IMP dehydrogenase gene

We determined the chromosomal localization and structure of the gene encoding human type II inosine 5{prime}-monophosphate dehydrogenase (IMPDH, EC 1.1.1.205), an enzyme associated with cellular proliferation, malignant transformation, and differentiation. Using polymerase chain reaction (PCR) primers specific for type II IMPDH, we screened a panel of human-Chinese hamster cell somatic hybrids and a separate deletion panel of chromosome 3 hybrids and localized the gene to 3p21.1{yields}p24.2. Two overlapping yeast artificial chromosome clones containing the full gene for type II IMPDH were isolated and a physical map of 117 kb of human genomic DNA in this region of chromosome 3 was constructed. The gene for type II IMPDH was localized and oriented on this map and found to span no more than 12.5 kb.
Authors:
;  [1] ;  [2] ; ;  [3]
  1. Chicago Univ., IL (United States). Dept. of Molecular Genetics and Cell Biology|[Argonne National Lab., IL (United States)
  2. Argonne National Lab., IL (United States)
  3. Colorado Univ., Denver, CO (United States). Health Sciences Center
Publication Date:
OSTI Identifier:
10148872
Report Number(s):
ANL/BIM/PP--77638
ON: DE94011499; CNN: Grant HG00358
DOE Contract Number:
W-31109-ENG-38
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: [1994]
Research Org:
Argonne National Lab., IL (United States)
Sponsoring Org:
USDOE, Washington, DC (United States);Department of Health and Human Services, Washington, DC (United States)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; NUCLEOTIDE DEHYDROGENASES; GENETIC MAPPING; GENE AMPLIFICATION; MAN; HUMAN CHROMOSOME 3 550400; 550200; GENETICS; BIOCHEMISTRY