Self-assembled virus-membrane complexes

Yang, Lihua; Liang, Hongjun; Angelini, Thomas; Butler, John; Coridan, Robert; Tang, Jay; Wong, Gerard

doi:10.1038/nmat1195

Title: Self-assembled virus-membrane complexes

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Abstract

Anionic polyelectrolytes and cationic lipid membranes can self-assemble into lamellar structures ranging from alternating layers of membranes and polyelectrolytes to 'missing layer' superlattice structures. We show that these structural differences can be understood in terms of the surface-charge-density mismatch between the polyelectrolyte and membrane components by examining complexes between cationic membranes and highly charged M13 viruses, a system that allowed us to vary the polyelectrolyte diameter independently of the charge density. Such virus-membrane complexes have pore sizes that are about ten times larger in area than DNA-membrane complexes, and can be used to package and organize large functional molecules; correlated arrays of Ru(bpy){sub 3}{sup 2+} macroionic dyes have been directly observed within the virus-membrane complexes using an electron-density reconstruction. These observations elucidate fundamental design rules for rational control of self-assembled polyelectrolyte-membrane structures, which have applications ranging from non-viral gene therapy to biomolecular templates for nanofabrication.

Authors:

Yang, Lihua; Liang, Hongjun; Angelini, Thomas; Butler, John; Coridan, Robert; Tang, Jay; Wong, Gerard ^[1]

UIUC

Publication Date:: Tue Nov 16 00:00:00 EST 2010

Research Org.:: Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1008736

Resource Type:: Journal Article

Journal Name:: Nat. Mater.

Additional Journal Information:: Journal Volume: 3; Journal Issue: 09, 2004

Country of Publication:: United States

Language:: ENGLISH

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; ELECTROLYTES; FABRICATION; CHARGE DENSITY; DESIGN; DYES; ELECTRON DENSITY; GENE THERAPY; LIPIDS; MEMBRANES; SUPERLATTICES; VIRUSES

Citation Formats


                    Yang, Lihua, Liang, Hongjun, Angelini, Thomas, Butler, John, Coridan, Robert, Tang, Jay, Wong, Gerard, and Brown). Self-assembled virus-membrane complexes.  United States: N. p., 2010. 
        Web.  doi:10.1038/nmat1195.

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                    Yang, Lihua, Liang, Hongjun, Angelini, Thomas, Butler, John, Coridan, Robert, Tang, Jay, Wong, Gerard, & Brown). Self-assembled virus-membrane complexes.  United States.  https://doi.org/10.1038/nmat1195

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                    Yang, Lihua, Liang, Hongjun, Angelini, Thomas, Butler, John, Coridan, Robert, Tang, Jay, Wong, Gerard, and Brown). 2010.  
        "Self-assembled virus-membrane complexes".  United States.  https://doi.org/10.1038/nmat1195.

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@article{osti_1008736,

  title        = {Self-assembled virus-membrane complexes},

  author       = {Yang, Lihua and Liang, Hongjun and Angelini, Thomas and Butler, John and Coridan, Robert and Tang, Jay and Wong, Gerard and Brown)},

  abstractNote = {Anionic polyelectrolytes and cationic lipid membranes can self-assemble into lamellar structures ranging from alternating layers of membranes and polyelectrolytes to 'missing layer' superlattice structures. We show that these structural differences can be understood in terms of the surface-charge-density mismatch between the polyelectrolyte and membrane components by examining complexes between cationic membranes and highly charged M13 viruses, a system that allowed us to vary the polyelectrolyte diameter independently of the charge density. Such virus-membrane complexes have pore sizes that are about ten times larger in area than DNA-membrane complexes, and can be used to package and organize large functional molecules; correlated arrays of Ru(bpy){sub 3}{sup 2+} macroionic dyes have been directly observed within the virus-membrane complexes using an electron-density reconstruction. These observations elucidate fundamental design rules for rational control of self-assembled polyelectrolyte-membrane structures, which have applications ranging from non-viral gene therapy to biomolecular templates for nanofabrication.},

  doi          = {10.1038/nmat1195},

  url          = {https://www.osti.gov/biblio/1008736},
  journal      = {Nat. Mater.},
number       = 09, 2004,

  volume       = 3,

  place        = {United States},

  year         = {Tue Nov 16 00:00:00 EST 2010},

  month        = {Tue Nov 16 00:00:00 EST 2010}

}

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