skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Determining the Structure of an Unliganded and Fully Glycosylated SIV gp120 Envelope Glycoprotein

Abstract

HIV/SIV envelope glycoproteins mediate the first steps in viral infection. They are trimers of a membrane-anchored polypeptide chain, cleaved into two fragments known as gp120 and gp41. The structure of HIV gp120 bound with receptor (CD4) has been known for some time. We have now determined the structure of a fully glycosylated SIV gp120 envelope glycoprotein in an unliganded conformation by X-ray crystallography at 4.0 {angstrom} resolution. We describe here our experimental and computational approaches, which may be relevant to other resolution-limited crystallographic problems. Key issues were attention to details of beam geometry mandated by small, weakly diffracting crystals, and choice of strategies for phase improvement, starting with two isomorphous derivatives and including multicrystal averaging. We validated the structure by analyzing composite omit maps, averaged among three distinct crystal lattices, and by calculating model-based, SeMet anomalous difference maps. There are at least four ordered sugars on many of the thirteen oligosaccharides.

Authors:
; ; ; ; ;  [1]
  1. Harvard-Med
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
USDOE
OSTI Identifier:
1008637
Resource Type:
Journal Article
Journal Name:
Structure
Additional Journal Information:
Journal Volume: 13; Journal Issue: (2) ; 2005
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; AIDS VIRUS; CRYSTAL LATTICES; CRYSTALLOGRAPHY; GEOMETRY; GLYCOPROTEINS; OLIGOSACCHARIDES; POLYPEPTIDES; RESOLUTION; SACCHARIDES

Citation Formats

Chen, Bing, Vogan, Erik M, Gong, Haiyun, Skehel, John J, Wiley, Don C, Harrison, Stephen C, and NIMR). Determining the Structure of an Unliganded and Fully Glycosylated SIV gp120 Envelope Glycoprotein. United States: N. p., 2010. Web.
Chen, Bing, Vogan, Erik M, Gong, Haiyun, Skehel, John J, Wiley, Don C, Harrison, Stephen C, & NIMR). Determining the Structure of an Unliganded and Fully Glycosylated SIV gp120 Envelope Glycoprotein. United States.
Chen, Bing, Vogan, Erik M, Gong, Haiyun, Skehel, John J, Wiley, Don C, Harrison, Stephen C, and NIMR). 2010. "Determining the Structure of an Unliganded and Fully Glycosylated SIV gp120 Envelope Glycoprotein". United States.
@article{osti_1008637,
title = {Determining the Structure of an Unliganded and Fully Glycosylated SIV gp120 Envelope Glycoprotein},
author = {Chen, Bing and Vogan, Erik M and Gong, Haiyun and Skehel, John J and Wiley, Don C and Harrison, Stephen C and NIMR)},
abstractNote = {HIV/SIV envelope glycoproteins mediate the first steps in viral infection. They are trimers of a membrane-anchored polypeptide chain, cleaved into two fragments known as gp120 and gp41. The structure of HIV gp120 bound with receptor (CD4) has been known for some time. We have now determined the structure of a fully glycosylated SIV gp120 envelope glycoprotein in an unliganded conformation by X-ray crystallography at 4.0 {angstrom} resolution. We describe here our experimental and computational approaches, which may be relevant to other resolution-limited crystallographic problems. Key issues were attention to details of beam geometry mandated by small, weakly diffracting crystals, and choice of strategies for phase improvement, starting with two isomorphous derivatives and including multicrystal averaging. We validated the structure by analyzing composite omit maps, averaged among three distinct crystal lattices, and by calculating model-based, SeMet anomalous difference maps. There are at least four ordered sugars on many of the thirteen oligosaccharides.},
doi = {},
url = {https://www.osti.gov/biblio/1008637}, journal = {Structure},
number = (2) ; 2005,
volume = 13,
place = {United States},
year = {Tue Jul 13 00:00:00 EDT 2010},
month = {Tue Jul 13 00:00:00 EDT 2010}
}