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Title: Enclosed chambers for humidity control and sample containment in fiber diffraction

A chamber and stretch frame for making fibers for diffraction is described. The chamber is made from a simple plastic cuvette with silicon nitride windows. It is suitable for maintaining constant humidity during fiber drying and data collection, and allows stretching of the fiber and exposure to magnetic fields during sample preparation. If necessary, it provides primary containment for toxic and infectious biological materials. The chamber has been used in fiber diffraction experiments with filamentous plant viruses and a yeast prion protein, and is shown to produce excellent orientation and to maintain hydration and order at the molecular level.
Authors:
; ; ; ;  [1] ;  [2]
  1. (Vanderbilt)
  2. (
Publication Date:
OSTI Identifier:
1006921
Resource Type:
Journal Article
Resource Relation:
Journal Name: J. Appl. Crystallogr.; Journal Volume: 41; Journal Issue: (1) ; 02, 2008
Research Org:
Advanced Photon Source (APS), Argonne National Laboratory (ANL), Argonne, IL (US)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
ENGLISH
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES; SAMPLE HOLDERS; DESIGN; FIBERS; DIFFRACTION; HUMIDITY CONTROL; SAMPLE PREPARATION; BIOLOGICAL MATERIALS; CONTAINMENT; HYDRATION; PLANTS; SILICON NITRIDES; VIRUSES; YEASTS