Imaging of cochlear tissue with a grating interferometer and hard X-rays
Abstract
This article addresses an important current development in medical and biological imaging: the possibility of imaging soft tissue at resolutions in the micron range using hard X-rays. Challenging environments, including the cochlea, require the imaging of soft tissue structure surrounded by bone. We demonstrate that cochlear soft tissue structures can be imaged with hard X-ray phase contrast. Furthermore, we show that only a thin slice of the tissue is required to introduce a large phase shift. It is likely that the phase contrast image of the soft tissue structures is sufficient to image the structures even if surrounded by bone. For the present set of experiments, structures with low-absorption contrast have been visualized using in-line phase contrast imaging and a grating interferometer. The experiments have been performed at the Advanced Photon Source at Argonne National Laboratories, a third generation source of synchrotron radiation. The source provides highly coherent X-ray radiation with high-photon flux (>10{sup 12} photons/s) at high-photon energies (5-70 keV). Radiographic and light microscopy images of the gerbil cochlear slice samples were compared. It has been determined that a 20-{micro}m thick tissue slice induces a phase shift between 1/3{pi} and 2/3{pi}.
- Authors:
- Publication Date:
- Research Org.:
- Argonne National Lab. (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1006019
- Resource Type:
- Journal Article
- Journal Name:
- Microsc. Res. Techniq.
- Additional Journal Information:
- Journal Volume: 72; Journal Issue: (12) ; 2009; Journal ID: ISSN 1059-910X
- Country of Publication:
- United States
- Language:
- ENGLISH
- Subject:
- 43 PARTICLE ACCELERATORS; ADVANCED PHOTON SOURCE; GERBILS; INTERFEROMETERS; MICROSCOPY; PHASE SHIFT; RADIATIONS; SYNCHROTRON RADIATION
Citation Formats
Richter, Claus-Peter, Shintani-Smith, Stephanie, Fishman, Andrew, David, Christian, Robinson, Ian, Rau, Christoph, PSI), NWU), and UCL). Imaging of cochlear tissue with a grating interferometer and hard X-rays. United States: N. p., 2010.
Web. doi:10.1002/jemt.20728.
Richter, Claus-Peter, Shintani-Smith, Stephanie, Fishman, Andrew, David, Christian, Robinson, Ian, Rau, Christoph, PSI), NWU), & UCL). Imaging of cochlear tissue with a grating interferometer and hard X-rays. United States. https://doi.org/10.1002/jemt.20728
Richter, Claus-Peter, Shintani-Smith, Stephanie, Fishman, Andrew, David, Christian, Robinson, Ian, Rau, Christoph, PSI), NWU), and UCL). 2010.
"Imaging of cochlear tissue with a grating interferometer and hard X-rays". United States. https://doi.org/10.1002/jemt.20728.
@article{osti_1006019,
title = {Imaging of cochlear tissue with a grating interferometer and hard X-rays},
author = {Richter, Claus-Peter and Shintani-Smith, Stephanie and Fishman, Andrew and David, Christian and Robinson, Ian and Rau, Christoph and PSI) and NWU) and UCL)},
abstractNote = {This article addresses an important current development in medical and biological imaging: the possibility of imaging soft tissue at resolutions in the micron range using hard X-rays. Challenging environments, including the cochlea, require the imaging of soft tissue structure surrounded by bone. We demonstrate that cochlear soft tissue structures can be imaged with hard X-ray phase contrast. Furthermore, we show that only a thin slice of the tissue is required to introduce a large phase shift. It is likely that the phase contrast image of the soft tissue structures is sufficient to image the structures even if surrounded by bone. For the present set of experiments, structures with low-absorption contrast have been visualized using in-line phase contrast imaging and a grating interferometer. The experiments have been performed at the Advanced Photon Source at Argonne National Laboratories, a third generation source of synchrotron radiation. The source provides highly coherent X-ray radiation with high-photon flux (>10{sup 12} photons/s) at high-photon energies (5-70 keV). Radiographic and light microscopy images of the gerbil cochlear slice samples were compared. It has been determined that a 20-{micro}m thick tissue slice induces a phase shift between 1/3{pi} and 2/3{pi}.},
doi = {10.1002/jemt.20728},
url = {https://www.osti.gov/biblio/1006019},
journal = {Microsc. Res. Techniq.},
issn = {1059-910X},
number = (12) ; 2009,
volume = 72,
place = {United States},
year = {Thu Jan 28 00:00:00 EST 2010},
month = {Thu Jan 28 00:00:00 EST 2010}
}