Oscillatory phase separation during expulsion of daughter GUVs
Abstract
A video assembled from time-lapse fluorescence images of phase-separating GUVs containing internal ‘organelle’ vesicles. GUVs consisting of POPC:SM:Ch (2:2:1) labeled with 0.5 mol% Sphingomyelin-Atto647N (SM-647N) (pseudo-colored green), encapsulate 1 M sucrose, and diluted in MilliQ water at 25°C. The video reveals shifting patterns of osmotic pressure and tension during expulsion of the internal vesicles after an osmotic differential had been established. Key steps include (A) a homogeneous, flaccid mother vesicle encapsulating tense daughter vesicles, at a time point prior to vesicle expulsion; (B) just after expulsion, the daughter GUV remains tense exhibiting oscillatory phase separation, while the mother GUV is left deflated and homogenous due to the sudden loss of volume; (C) The mother GUV subsequently becomes inflated by influx of water; and (D) the mother GUV begins to exhibit oscillatory phase separation.
- Authors:
- Publication Date:
- Research Org.:
- ELIFE-C (eLife Sciences Publications, Ltd.)
- OSTI Identifier:
- 1806205
- Resource Type:
- Multimedia
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; GUV; RESEARCH ARTICLE; CELL BIOLOGY; GIANT PHOSPHOLIPID VESICLE; LIPID RAFT; PHASE SEPARATION; PRIMITIVE OSMOREGULATION; COMPARTMENTALIZATION
Citation Formats
Oglecka, Kamila, Rangamani, Padmini, Liedberg, Bo, Kraut, Rachel S, and Parikh, Atul N. Oscillatory phase separation during expulsion of daughter GUVs. United States: N. p., 2014.
Web. doi:10.7554/eLife.03695.018.
Oglecka, Kamila, Rangamani, Padmini, Liedberg, Bo, Kraut, Rachel S, & Parikh, Atul N. Oscillatory phase separation during expulsion of daughter GUVs. United States. doi:https://doi.org/10.7554/eLife.03695.018
Oglecka, Kamila, Rangamani, Padmini, Liedberg, Bo, Kraut, Rachel S, and Parikh, Atul N. Wed .
"Oscillatory phase separation during expulsion of daughter GUVs". United States. doi:https://doi.org/10.7554/eLife.03695.018. https://www.osti.gov/servlets/purl/1806205.
@article{osti_1806205,
title = {Oscillatory phase separation during expulsion of daughter GUVs},
author = {Oglecka, Kamila and Rangamani, Padmini and Liedberg, Bo and Kraut, Rachel S and Parikh, Atul N},
abstractNote = {A video assembled from time-lapse fluorescence images of phase-separating GUVs containing internal ‘organelle’ vesicles. GUVs consisting of POPC:SM:Ch (2:2:1) labeled with 0.5 mol% Sphingomyelin-Atto647N (SM-647N) (pseudo-colored green), encapsulate 1 M sucrose, and diluted in MilliQ water at 25°C. The video reveals shifting patterns of osmotic pressure and tension during expulsion of the internal vesicles after an osmotic differential had been established. Key steps include (A) a homogeneous, flaccid mother vesicle encapsulating tense daughter vesicles, at a time point prior to vesicle expulsion; (B) just after expulsion, the daughter GUV remains tense exhibiting oscillatory phase separation, while the mother GUV is left deflated and homogenous due to the sudden loss of volume; (C) The mother GUV subsequently becomes inflated by influx of water; and (D) the mother GUV begins to exhibit oscillatory phase separation.},
doi = {10.7554/eLife.03695.018},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Oct 01 00:00:00 EDT 2014},
month = {Wed Oct 01 00:00:00 EDT 2014}
}