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Title: GUVs prepared by gentle hydration reproduce the oscillatory domain dynamics

Abstract

GUVs prepared by ‘gentle hydration’ consisting of POPC:SM:Ch (1:1:1) labeled with 0.5 mol% Rho-DPPE (pseudo-colored blue), encapsulating 200 mM sucrose. Hypotonic conditions are established by dilution in deionized water at 25°C. Vesicles exhibit osmotic swelling and oscillatory domain dynamics comparable to that seen for electroformed GUVs under the same conditions.

Authors:
; ; ; ;
Publication Date:
Research Org.:
ELIFE-C (eLife Sciences Publications, Ltd.)
OSTI Identifier:
1806200
Resource Type:
Multimedia
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GUV; RESEARCH ARTICLE; CELL BIOLOGY; GIANT PHOSPHOLIPID VESICLE; LIPID RAFT; PHASE SEPARATION; PRIMITIVE OSMOREGULATION; COMPARTMENTALIZATION

Citation Formats

Oglecka, Kamila, Rangamani, Padmini, Liedberg, Bo, Kraut, Rachel S, and Parikh, Atul N. GUVs prepared by gentle hydration reproduce the oscillatory domain dynamics. United States: N. p., 2014. Web. doi:10.7554/eLife.03695.011.
Oglecka, Kamila, Rangamani, Padmini, Liedberg, Bo, Kraut, Rachel S, & Parikh, Atul N. GUVs prepared by gentle hydration reproduce the oscillatory domain dynamics. United States. doi:https://doi.org/10.7554/eLife.03695.011
Oglecka, Kamila, Rangamani, Padmini, Liedberg, Bo, Kraut, Rachel S, and Parikh, Atul N. Wed . "GUVs prepared by gentle hydration reproduce the oscillatory domain dynamics". United States. doi:https://doi.org/10.7554/eLife.03695.011. https://www.osti.gov/servlets/purl/1806200.
@article{osti_1806200,
title = {GUVs prepared by gentle hydration reproduce the oscillatory domain dynamics},
author = {Oglecka, Kamila and Rangamani, Padmini and Liedberg, Bo and Kraut, Rachel S and Parikh, Atul N},
abstractNote = {GUVs prepared by ‘gentle hydration’ consisting of POPC:SM:Ch (1:1:1) labeled with 0.5 mol% Rho-DPPE (pseudo-colored blue), encapsulating 200 mM sucrose. Hypotonic conditions are established by dilution in deionized water at 25°C. Vesicles exhibit osmotic swelling and oscillatory domain dynamics comparable to that seen for electroformed GUVs under the same conditions.},
doi = {10.7554/eLife.03695.011},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {10}
}