An automated liquid jet for fluorescence dosimetry and microsecond radiolytic labeling of proteins
Abstract
X-ray radiolytic labeling uses broadband X-rays for in situ hydroxyl radical labeling to map protein interactions and conformation. High flux density beams are essential to overcome radical scavengers. However, conventional sample delivery environments, such as capillary flow, limit the use of a fully unattenuated focused broadband beam. An alternative is to use a liquid jet, and we have previously demonstrated that use of this form of sample delivery can increase labeling by tenfold at an unfocused X-ray source. Here we report the first use of a liquid jet for automated inline quantitative fluorescence dosage characterization and sample exposure at a high flux density microfocused synchrotron beamline. Our approach enables exposure times in single-digit microseconds while retaining a high level of side-chain labeling. This development significantly boosts the method’s overall effectiveness and efficiency, generates high-quality data, and opens up the arena for high throughput and ultrafast time-resolved in situ hydroxyl radical labeling.
- Authors:
-
- Sonoma State University, CA (United States)
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Case Western Reserve University, Cleveland, OH (United States)
- University of California, Berkeley, CA (United States)
- Michigan State University, East Lansing, MI (United States)
- SLAC National Accelerator Laboratory, Menlo Park, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF); Case Western Reserve University; National Institutes of Health (NIH)
- OSTI Identifier:
- 1907590
- Grant/Contract Number:
- AC02-05CH11231; SC0012704; DBI-1228549; P30-EB-009998; 1R01GM126218; 1R21AG072478; SC0020606; GM050945
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Communications Biology
- Additional Journal Information:
- Journal Volume: 5; Journal Issue: 1; Journal ID: ISSN 2399-3642
- Publisher:
- Springer Nature
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; biological techniques; biophysical methods; structural biology
Citation Formats
Rosi, Matthew, Russell, Brandon, Kristensen, Line G., Farquhar, Erik R., Jain, Rohit, Abel, Donald, Sullivan, Michael, Costello, Shawn M., Dominguez-Martin, Maria Agustina, Chen, Yan, Marqusee, Susan, Petzold, Christopher J., Kerfeld, Cheryl A., DePonte, Daniel P., Farahmand, Farid, Gupta, Sayan, and Ralston, Corie Y. An automated liquid jet for fluorescence dosimetry and microsecond radiolytic labeling of proteins. United States: N. p., 2022.
Web. doi:10.1038/s42003-022-03775-1.
Rosi, Matthew, Russell, Brandon, Kristensen, Line G., Farquhar, Erik R., Jain, Rohit, Abel, Donald, Sullivan, Michael, Costello, Shawn M., Dominguez-Martin, Maria Agustina, Chen, Yan, Marqusee, Susan, Petzold, Christopher J., Kerfeld, Cheryl A., DePonte, Daniel P., Farahmand, Farid, Gupta, Sayan, & Ralston, Corie Y. An automated liquid jet for fluorescence dosimetry and microsecond radiolytic labeling of proteins. United States. https://doi.org/10.1038/s42003-022-03775-1
Rosi, Matthew, Russell, Brandon, Kristensen, Line G., Farquhar, Erik R., Jain, Rohit, Abel, Donald, Sullivan, Michael, Costello, Shawn M., Dominguez-Martin, Maria Agustina, Chen, Yan, Marqusee, Susan, Petzold, Christopher J., Kerfeld, Cheryl A., DePonte, Daniel P., Farahmand, Farid, Gupta, Sayan, and Ralston, Corie Y. Thu .
"An automated liquid jet for fluorescence dosimetry and microsecond radiolytic labeling of proteins". United States. https://doi.org/10.1038/s42003-022-03775-1. https://www.osti.gov/servlets/purl/1907590.
@article{osti_1907590,
title = {An automated liquid jet for fluorescence dosimetry and microsecond radiolytic labeling of proteins},
author = {Rosi, Matthew and Russell, Brandon and Kristensen, Line G. and Farquhar, Erik R. and Jain, Rohit and Abel, Donald and Sullivan, Michael and Costello, Shawn M. and Dominguez-Martin, Maria Agustina and Chen, Yan and Marqusee, Susan and Petzold, Christopher J. and Kerfeld, Cheryl A. and DePonte, Daniel P. and Farahmand, Farid and Gupta, Sayan and Ralston, Corie Y.},
abstractNote = {X-ray radiolytic labeling uses broadband X-rays for in situ hydroxyl radical labeling to map protein interactions and conformation. High flux density beams are essential to overcome radical scavengers. However, conventional sample delivery environments, such as capillary flow, limit the use of a fully unattenuated focused broadband beam. An alternative is to use a liquid jet, and we have previously demonstrated that use of this form of sample delivery can increase labeling by tenfold at an unfocused X-ray source. Here we report the first use of a liquid jet for automated inline quantitative fluorescence dosage characterization and sample exposure at a high flux density microfocused synchrotron beamline. Our approach enables exposure times in single-digit microseconds while retaining a high level of side-chain labeling. This development significantly boosts the method’s overall effectiveness and efficiency, generates high-quality data, and opens up the arena for high throughput and ultrafast time-resolved in situ hydroxyl radical labeling.},
doi = {10.1038/s42003-022-03775-1},
journal = {Communications Biology},
number = 1,
volume = 5,
place = {United States},
year = {Thu Aug 25 00:00:00 EDT 2022},
month = {Thu Aug 25 00:00:00 EDT 2022}
}
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