Synthetic antibacterial minerals: harnessing a natural geochemical reaction to combat antibiotic resistance
Abstract
The overuse of antibiotics in clinical and livestock settings is accelerating the selection of multidrug resistant bacterial pathogens. Antibiotic resistant bacteria result in increased mortality and financial strain on the health care and livestock industry. The development of new antibiotics has stalled, and novel strategies are needed as we enter the age of antibiotic resistance. Certain naturally occurring clays have been shown to have antimicrobial properties and kill antibiotic resistant bacteria. Harnessing the activity of compounds within these clays that harbor antibiotic properties offers new therapeutic opportunities for fighting the potentially devastating effects of the post antibiotic era. However, natural samples are highly heterogenous and exhibit variable antibacterial effectiveness, therefore synthesizing minerals of high purity with reproducible antibacterial activity is needed. Here we describe for the first time synthetic smectite clay minerals and Fe-sulfide microspheres that reproduce the geochemical antibacterial properties observed in natural occurring clays. We show that these mineral formulations are effective at killing the ESKAPE pathogens (Enterococcus sp., Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter sp., Pseudomonas aeruginosa and Enterobacter sp.) by maintaining Fe2+ solubility and reactive oxygen species (ROS) production while buffering solution pH, unlike the application of metals alone. Our results represent the first step in utilizingmore »
- Publication Date:
- Research Org.:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA); USDOE Laboratory Directed Research and Development (LDRD) Program
- OSTI Identifier:
- 1844081
- Report Number(s):
- LLNL-JRNL-825820
Journal ID: ISSN 2045-2322; 1039949
- Grant/Contract Number:
- AC52-07NA27344; 19-LW-029
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Scientific Reports
- Additional Journal Information:
- Journal Volume: 12; Journal Issue: 1; Journal ID: ISSN 2045-2322
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 58 GEOSCIENCES; Antibiotic resistance; Antibacterial clay; Antibacterial mineral; Geochemistry
Citation Formats
Morrison, Keith D., Martin, Kelly A., Wimpenny, Josh B., and Loots, Gabriela G. Synthetic antibacterial minerals: harnessing a natural geochemical reaction to combat antibiotic resistance. United States: N. p., 2022.
Web. doi:10.1038/s41598-022-05303-x.
Morrison, Keith D., Martin, Kelly A., Wimpenny, Josh B., & Loots, Gabriela G. Synthetic antibacterial minerals: harnessing a natural geochemical reaction to combat antibiotic resistance. United States. https://doi.org/10.1038/s41598-022-05303-x
Morrison, Keith D., Martin, Kelly A., Wimpenny, Josh B., and Loots, Gabriela G. Mon .
"Synthetic antibacterial minerals: harnessing a natural geochemical reaction to combat antibiotic resistance". United States. https://doi.org/10.1038/s41598-022-05303-x. https://www.osti.gov/servlets/purl/1844081.
@article{osti_1844081,
title = {Synthetic antibacterial minerals: harnessing a natural geochemical reaction to combat antibiotic resistance},
author = {Morrison, Keith D. and Martin, Kelly A. and Wimpenny, Josh B. and Loots, Gabriela G.},
abstractNote = {The overuse of antibiotics in clinical and livestock settings is accelerating the selection of multidrug resistant bacterial pathogens. Antibiotic resistant bacteria result in increased mortality and financial strain on the health care and livestock industry. The development of new antibiotics has stalled, and novel strategies are needed as we enter the age of antibiotic resistance. Certain naturally occurring clays have been shown to have antimicrobial properties and kill antibiotic resistant bacteria. Harnessing the activity of compounds within these clays that harbor antibiotic properties offers new therapeutic opportunities for fighting the potentially devastating effects of the post antibiotic era. However, natural samples are highly heterogenous and exhibit variable antibacterial effectiveness, therefore synthesizing minerals of high purity with reproducible antibacterial activity is needed. Here we describe for the first time synthetic smectite clay minerals and Fe-sulfide microspheres that reproduce the geochemical antibacterial properties observed in natural occurring clays. We show that these mineral formulations are effective at killing the ESKAPE pathogens (Enterococcus sp., Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter sp., Pseudomonas aeruginosa and Enterobacter sp.) by maintaining Fe2+ solubility and reactive oxygen species (ROS) production while buffering solution pH, unlike the application of metals alone. Our results represent the first step in utilizing a geochemical process to treat antibiotic resistant topical or gastrointestinal infections in the age of antibiotic resistance.},
doi = {10.1038/s41598-022-05303-x},
journal = {Scientific Reports},
number = 1,
volume = 12,
place = {United States},
year = {Mon Jan 24 00:00:00 EST 2022},
month = {Mon Jan 24 00:00:00 EST 2022}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Influence of pH on wound-healing: a new perspective for wound-therapy?
journal, November 2006
- Schneider, Lars Alexander; Korber, Andreas; Grabbe, Stephan
- Archives of Dermatological Research, Vol. 298, Issue 9
pH in nature, humans and skin
journal, June 2018
- Proksch, Ehrhardt
- The Journal of Dermatology, Vol. 45, Issue 9
The Anatomy of an Antibacterial Clay Deposit: A New Economic Geology
journal, November 2017
- Morrison, Keith D.; Williams, Stanley N.; Williams, Lynda B.
- Economic Geology, Vol. 112, Issue 7
Rheological Properties of Organophilic Synthetic Hectorites and Saponites
journal, January 1989
- Iwasaki, Takashi
- Clays and Clay Minerals, Vol. 37, Issue 3
A simple and fast method for determining colony forming units
journal, October 2008
- Sieuwerts, S.; de Bok, F. A. M.; Mols, E.
- Letters in Applied Microbiology, Vol. 47, Issue 4
Fibroblast proliferation due to exposure to a platelet concentrate in vitro is pH dependent
journal, September 2002
- Liu, Yawei; Kalén, Anders; Risto, Olof
- Wound Repair and Regeneration, Vol. 10, Issue 5
Effect of capping ligands on the optical properties and electronic energies of iron pyrite FeS2 nanocrystals and solid thin films
journal, July 2016
- Zhai, Guangmei; Xie, Rongwei; Wang, Heng
- Journal of Alloys and Compounds, Vol. 674
Topical acidification promotes healing of experimental deep partial thickness skin burns: a randomized double-blind preliminary study
journal, December 1985
- Kaufman, T.; Eichenlaub, E. H.; Angel, M. F.
- Burns, Vol. 12, Issue 2
Chemical Characterisation of the USGS Reference Glasses GSA-1G, GSC-1G, GSD-1G, GSE-1G, BCR-2G, BHVO-2G and BIR-1G Using EPMA, ID-TIMS, ID-ICP-MS and LA-ICP-MS
journal, November 2005
- Jochum, Klaus Peter; Willbold, Matthias; Raczek, Ingrid
- Geostandards and Geoanalytical Research, Vol. 29, Issue 3
The Effect of pH on the Extracellular Matrix and Biofilms
journal, July 2015
- Jones, Eleri M.; Cochrane, Christine A.; Percival, Steven L.
- Advances in Wound Care, Vol. 4, Issue 7
ROS in gastrointestinal inflammation: Rescue Or Sabotage?: ROS and GI inflammation
journal, March 2016
- Aviello, G.; Knaus, Ug
- British Journal of Pharmacology, Vol. 174, Issue 12
Antimicrobial Actions of Reactive Oxygen Species
journal, November 2011
- Fang, Ferric C.
- mBio, Vol. 2, Issue 5
Mouse Model of Enteropathogenic Escherichia coli Infection
journal, January 2005
- Savkovic, S. D.; Villanueva, J.; Turner, J. R.
- Infection and Immunity, Vol. 73, Issue 2
A Device to Predict Short-Term Healing Outcome of Chronic Wounds
journal, June 2020
- Vu, Hong; Nair, Ashwin; Tran, Lan
- Advances in Wound Care, Vol. 9, Issue 6
Optimization of single plate-serial dilution spotting (SP-SDS) with sample anchoring as an assured method for bacterial and yeast cfu enumeration and single colony isolation from diverse samples
journal, December 2015
- Thomas, Pious; Sekhar, Aparna C.; Upreti, Reshmi
- Biotechnology Reports, Vol. 8
O 2 availability impacts iron homeostasis in Escherichia coli
journal, October 2017
- Beauchene, Nicole A.; Mettert, Erin L.; Moore, Laura J.
- Proceedings of the National Academy of Sciences, Vol. 114, Issue 46
Natural resistance, iron and infection: a challenge for clinical medicine
journal, March 2006
- Bullen, John J.; Rogers, Henry J.; Spalding, Paul B.
- Journal of Medical Microbiology, Vol. 55, Issue 3
Acidic Mine Drainage: The Rate-Determining Step
journal, February 1970
- Singer, P. C.; Stumm, W.
- Science, Vol. 167, Issue 3921
Chemical Acidification of Wounds: An Adjuvant to Healing and the Unfavorable Action of Alkalinity and Ammonia
journal, January 1973
- Leveen, Harry H.; Falk, Gerald; Borek, Blanche
- Annals of Surgery, Vol. 178, Issue 6
Comparison of pyrite (FeS2) synthesis mechanisms to reproduce natural FeS2 nanoparticles found at hydrothermal vents
journal, November 2013
- Gartman, Amy; Luther, George W.
- Geochimica et Cosmochimica Acta, Vol. 120
What Makes a Natural Clay Antibacterial?
journal, April 2011
- Williams, Lynda B.; Metge, David W.; Eberl, Dennis D.
- Environmental Science & Technology, Vol. 45, Issue 8
Unearthing the Antibacterial Mechanism of Medicinal Clay: A Geochemical Approach to Combating Antibiotic Resistance
journal, January 2016
- Morrison, Keith D.; Misra, Rajeev; Williams, Lynda B.
- Scientific Reports, Vol. 6, Issue 1
Baseline Studies of the Clay Minerals Society Source Clays: Layer-Charge Determination and Characteristics of those Minerals Containing 2:1 Layers
journal, January 2001
- Mermut, Ahmet R.
- Clays and Clay Minerals, Vol. 49, Issue 5
Preparation of hectorite clays utilizing organic and organometallic complexes during hydrothermal crystallization
journal, July 1992
- Carrado, Kathleen A.
- Industrial & Engineering Chemistry Research, Vol. 31, Issue 7
Role of hydrogen peroxide and hydroxyl radical in pyrite oxidation by molecular oxygen
journal, September 2010
- Schoonen, Martin A. A.; Harrington, Andrea D.; Laffers, Richard
- Geochimica et Cosmochimica Acta, Vol. 74, Issue 17
Mineralogical variables that control the antibacterial effectiveness of a natural clay deposit
journal, November 2013
- Morrison, Keith D.; Underwood, Jennifer C.; Metge, David W.
- Environmental Geochemistry and Health, Vol. 36, Issue 4
Dermal Wound Healing Is Subject to Redox Control
journal, January 2006
- Roy, Sashwati; Khanna, Savita; Nallu, Kishore
- Molecular Therapy, Vol. 13, Issue 1
Natural skin surface pH is on average below 5, which is beneficial for its resident flora
journal, October 2006
- Lambers, H.; Piessens, S.; Bloem, A.
- International Journal of Cosmetic Science, Vol. 28, Issue 5
On the Problems of Total Specific Surface Area and Cation Exchange Capacity Measurements in Organic-Rich Sedimentary Rocks
journal, August 2012
- Derkowski, Arkadiusz; Bristow, Thomas F.
- Clays and Clay Minerals, Vol. 60, Issue 4
Quantifying hydrogen peroxide in iron-containing solutions using leuco crystal violet
journal, January 2005
- Cohn, Corey A.
- Geochemical Transactions, Vol. 6, Issue 3