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Title: Rosette core fungal resistance in Arabidopsis thaliana

Abstract

Main conclusion: Unlike rosette leaves, the mature Arabidopsis rosette core can display full resistance to Botrytis cinerea revealing the importance for spatial and developmental aspects of plant fungal resistance. Abstract: Arabidopsis thaliana is a model host to investigate plant defense against fungi. However, many of the reports investigating Arabidopsis fungal defense against the necrotrophic fungus, Botrytis cinerea, utilize rosette leaves as host tissue. Here we report organ-dependent differences in B. cinerea resistance of Arabidopsis. Although wild-type Arabidopsis rosette leaves mount a jasmonate-dependent defense that slows fungal growth, this defense is incapable of resisting fungal devastation. In contrast, as the fungus spreads through infected leaf petioles towards the plant center, or rosette core, there is a jasmonate- and age-dependent fungal penetration blockage into the rosette core. In this paper, we report evidence for induced and preformed resistance in the rosette core, as direct rosette core inoculation can also result in resistance, but at a lower penetrance relative to infections that approach the core from infected leaf petioles. The Arabidopsis rosette core displays a distinct transcriptome relative to other plant organs, and BLADE ON PETIOLE (BOP) transcripts are abundant in the rosette core. The BOP genes, with known roles in abscission zonemore » formation, are required for full Arabidopsis rosette core B. cinerea resistance, suggesting a possible role for BOP-dependent modifications that may help to restrict fungal susceptibility of the rosette core. Finally, we demonstrate that cabbage and cauliflower, common Brassicaceae crops, also display leaf susceptibility and rosette core resistance to B. cinerea that can involve leaf abscission. Thus, spatial and developmental aspects of plant host resistance play critical roles in resistance to necrotrophic fungal pathogens and are important to our understanding of plant defense mechanisms.« less

Authors:
ORCiD logo [1];  [1];  [1];  [1];  [2];  [2];  [3];  [3];  [2]; ORCiD logo [2];  [1];  [4]; ORCiD logo [1]
  1. Rice Univ., Houston, TX (United States)
  2. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  3. Texas A & M Univ., College Station, TX (United States)
  4. National Chung Hsing Univ., Taichung (Taiwan)
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
Sponsoring Org.:
USDOE; China Scholarship Council (CSC)
OSTI Identifier:
1842955
Alternate Identifier(s):
OSTI ID: 1842956
Report Number(s):
PNNL-SA-147546
Journal ID: ISSN 0032-0935
Grant/Contract Number:  
AC05-76RL01830; 201406350018
Resource Type:
Accepted Manuscript
Journal Name:
Planta
Additional Journal Information:
Journal Volume: 250; Journal Issue: 6; Journal ID: ISSN 0032-0935
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Biotic stress; Botrytis cinerea; Jasmonate; Plant defense; RNA-seq

Citation Formats

Dai, Yanwan, Ogilvie, Huw A., Liu, Yuan, Huang, Michael, Markillie, Lye Meng, Mitchell, Hugh D., Borrego, Eli J., Kolomiets, Michael V., Gaffrey, Matthew J., Orr, Galya, Chehab, E. Wassim, Mao, Wan-Ting, and Braam, Janet. Rosette core fungal resistance in Arabidopsis thaliana. United States: N. p., 2019. Web. doi:10.1007/s00425-019-03273-5.
Dai, Yanwan, Ogilvie, Huw A., Liu, Yuan, Huang, Michael, Markillie, Lye Meng, Mitchell, Hugh D., Borrego, Eli J., Kolomiets, Michael V., Gaffrey, Matthew J., Orr, Galya, Chehab, E. Wassim, Mao, Wan-Ting, & Braam, Janet. Rosette core fungal resistance in Arabidopsis thaliana. United States. https://doi.org/10.1007/s00425-019-03273-5
Dai, Yanwan, Ogilvie, Huw A., Liu, Yuan, Huang, Michael, Markillie, Lye Meng, Mitchell, Hugh D., Borrego, Eli J., Kolomiets, Michael V., Gaffrey, Matthew J., Orr, Galya, Chehab, E. Wassim, Mao, Wan-Ting, and Braam, Janet. Mon . "Rosette core fungal resistance in Arabidopsis thaliana". United States. https://doi.org/10.1007/s00425-019-03273-5. https://www.osti.gov/servlets/purl/1842955.
@article{osti_1842955,
title = {Rosette core fungal resistance in Arabidopsis thaliana},
author = {Dai, Yanwan and Ogilvie, Huw A. and Liu, Yuan and Huang, Michael and Markillie, Lye Meng and Mitchell, Hugh D. and Borrego, Eli J. and Kolomiets, Michael V. and Gaffrey, Matthew J. and Orr, Galya and Chehab, E. Wassim and Mao, Wan-Ting and Braam, Janet},
abstractNote = {Main conclusion: Unlike rosette leaves, the mature Arabidopsis rosette core can display full resistance to Botrytis cinerea revealing the importance for spatial and developmental aspects of plant fungal resistance. Abstract: Arabidopsis thaliana is a model host to investigate plant defense against fungi. However, many of the reports investigating Arabidopsis fungal defense against the necrotrophic fungus, Botrytis cinerea, utilize rosette leaves as host tissue. Here we report organ-dependent differences in B. cinerea resistance of Arabidopsis. Although wild-type Arabidopsis rosette leaves mount a jasmonate-dependent defense that slows fungal growth, this defense is incapable of resisting fungal devastation. In contrast, as the fungus spreads through infected leaf petioles towards the plant center, or rosette core, there is a jasmonate- and age-dependent fungal penetration blockage into the rosette core. In this paper, we report evidence for induced and preformed resistance in the rosette core, as direct rosette core inoculation can also result in resistance, but at a lower penetrance relative to infections that approach the core from infected leaf petioles. The Arabidopsis rosette core displays a distinct transcriptome relative to other plant organs, and BLADE ON PETIOLE (BOP) transcripts are abundant in the rosette core. The BOP genes, with known roles in abscission zone formation, are required for full Arabidopsis rosette core B. cinerea resistance, suggesting a possible role for BOP-dependent modifications that may help to restrict fungal susceptibility of the rosette core. Finally, we demonstrate that cabbage and cauliflower, common Brassicaceae crops, also display leaf susceptibility and rosette core resistance to B. cinerea that can involve leaf abscission. Thus, spatial and developmental aspects of plant host resistance play critical roles in resistance to necrotrophic fungal pathogens and are important to our understanding of plant defense mechanisms.},
doi = {10.1007/s00425-019-03273-5},
journal = {Planta},
number = 6,
volume = 250,
place = {United States},
year = {Mon Sep 16 00:00:00 EDT 2019},
month = {Mon Sep 16 00:00:00 EDT 2019}
}

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