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Title: Polysaccharide niche partitioning of distinct Polaribacter clades during North Sea spring algal blooms

Abstract

Massive releases of organic substrates during marine algal blooms trigger growth of many clades of heterotrophic bacteria. Algal polysaccharides represent the most diverse and structurally complex class of these substrates, yet their role in shaping the microbial community composition is poorly understood. We investigated, whether polysaccharide utilization capabilities contribute to niche differentiation of Polaribacter spp. (class Flavobacteriia; known to include relevant polysaccharidedegraders) that were abundant during 2009–2012 spring algal blooms in the southern North Sea. We identified six distinct Polaribacter clades using phylogenetic and phylogenomic analyses, quantified their abundances via fluorescence in situ hybridization, compared metagenome-assembled genomes, and assessed in situ gene expression using metaproteomics. Four clades with distinct polysaccharide niches were dominating. Polaribacter 2-a comprised typical first responders featuring small genomes with limited polysaccharide utilization capacities. Polaribacter 3-a were abundant only in 2010 and possessed a distinct sulfated α-glucoronomannan degradation potential. Polaribacter 3-b responded late in blooms and had the capacity to utilize sulfated xylan. Polaribacter 1-a featured high numbers of glycan degradation genes and were particularly abundant following Chattonella algae blooms. These results support the hypothesis that sympatric Polaribacter clades occupy distinct glycan niches during North Sea spring algal blooms.

Authors:
ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1];  [1]; ORCiD logo [1]
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  1. Max Planck Inst. for Marine Microbiology, Bremen (Germany). Dept. of Molecular Ecology
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC)
Sponsoring Org.:
USDOE Office of Science (SC); German Research Foundation (DFG)
OSTI Identifier:
1816269
Grant/Contract Number:  
AC02-05CH11231; AM 73/9-1; TE 813/2-1; FU 627/2-1
Resource Type:
Accepted Manuscript
Journal Name:
The ISME Journal
Additional Journal Information:
Journal Volume: 14; Journal Issue: 6; Journal ID: ISSN 1751-7362
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; marine microbiology; metagenomics; microbial ecology

Citation Formats

Avcı, Burak, Krüger, Karen, Fuchs, Bernhard M., Teeling, Hanno, and Amann, Rudolf I. Polysaccharide niche partitioning of distinct Polaribacter clades during North Sea spring algal blooms. United States: N. p., 2020. Web. doi:10.1038/s41396-020-0601-y.
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Avcı, Burak, Krüger, Karen, Fuchs, Bernhard M., Teeling, Hanno, & Amann, Rudolf I. Polysaccharide niche partitioning of distinct Polaribacter clades during North Sea spring algal blooms. United States. https://doi.org/10.1038/s41396-020-0601-y
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Avcı, Burak, Krüger, Karen, Fuchs, Bernhard M., Teeling, Hanno, and Amann, Rudolf I. Wed . "Polysaccharide niche partitioning of distinct Polaribacter clades during North Sea spring algal blooms". United States. https://doi.org/10.1038/s41396-020-0601-y. https://www.osti.gov/servlets/purl/1816269.
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@article{osti_1816269,
title = {Polysaccharide niche partitioning of distinct Polaribacter clades during North Sea spring algal blooms},
author = {Avcı, Burak and Krüger, Karen and Fuchs, Bernhard M. and Teeling, Hanno and Amann, Rudolf I.},
abstractNote = {Massive releases of organic substrates during marine algal blooms trigger growth of many clades of heterotrophic bacteria. Algal polysaccharides represent the most diverse and structurally complex class of these substrates, yet their role in shaping the microbial community composition is poorly understood. We investigated, whether polysaccharide utilization capabilities contribute to niche differentiation of Polaribacter spp. (class Flavobacteriia; known to include relevant polysaccharidedegraders) that were abundant during 2009–2012 spring algal blooms in the southern North Sea. We identified six distinct Polaribacter clades using phylogenetic and phylogenomic analyses, quantified their abundances via fluorescence in situ hybridization, compared metagenome-assembled genomes, and assessed in situ gene expression using metaproteomics. Four clades with distinct polysaccharide niches were dominating. Polaribacter 2-a comprised typical first responders featuring small genomes with limited polysaccharide utilization capacities. Polaribacter 3-a were abundant only in 2010 and possessed a distinct sulfated α-glucoronomannan degradation potential. Polaribacter 3-b responded late in blooms and had the capacity to utilize sulfated xylan. Polaribacter 1-a featured high numbers of glycan degradation genes and were particularly abundant following Chattonella algae blooms. These results support the hypothesis that sympatric Polaribacter clades occupy distinct glycan niches during North Sea spring algal blooms.},
doi = {10.1038/s41396-020-0601-y},
journal = {The ISME Journal},
number = 6,
volume = 14,
place = {United States},
year = {Wed Feb 19 00:00:00 EST 2020},
month = {Wed Feb 19 00:00:00 EST 2020}
}
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https://doi.org/10.1038/s41396-020-0601-y
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