Gene Expression of Haloferax volcanii on Intermediate and Abundant Sources of Fixed Nitrogen
Abstract
Haloferax volcanii, a well-developed model archaeon for genomic, transcriptomic, and proteomic analyses, can grow on a defined medium of abundant and intermediate levels of fixed nitrogen. Here we report a global profiling of gene expression of H. volcanii grown on ammonium as an abundant source of fixed nitrogen compared to l-alanine, the latter of which exemplifies an intermediate source of nitrogen that can be obtained from dead cells in natural habitats. By comparing the two growth conditions, 30 genes were found to be differentially expressed, including 16 genes associated with amino acid metabolism and transport. The gene expression profiles contributed to mapping ammonium and l-alanine usage with respect to transporters and metabolic pathways. In addition, conserved DNA motifs were identified in the putative promoter regions and transcription factors were found to be in synteny with the differentially expressed genes, leading us to propose regulons of transcriptionally co-regulated operons. This study provides insight to how H. volcanii responds to and utilizes intermediate vs. abundant sources of fixed nitrogen for growth, with implications for conserved functions in related halophilic archaea.
- Authors:
-
[5]
- Duke Univ., Durham, NC (United States). Dept. of Biology
- Univ. of Florida, Gainesville, FL (United States). Inst. of Food and Agricultural Sciences. Dept. of Microbiology and Cell Science
- Duke Univ., Durham, NC (United States). Dept. of Biology; Duke Univ., Durham, NC (United States). Univ. Program in Genetics and Genomics
- Duke Univ., Durham, NC (United States). Dept. of Biology; Duke Univ., Durham, NC (United States). Univ. Program in Genetics and Genomics; Duke Univ., Durham, NC (United States). Center for Genomics and Computational Biology
- Univ. of Florida, Gainesville, FL (United States). Inst. of Food and Agricultural Sciences. Dept. of Microbiology and Cell Science; Univ. of Florida, Gainesville, FL (United States). Genetics Inst.
- Publication Date:
- Research Org.:
- Univ. of Florida, Gainesville, FL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division
- OSTI Identifier:
- 1800240
- Grant/Contract Number:
- FG02-05ER15650
- Resource Type:
- Accepted Manuscript
- Journal Name:
- International Journal of Molecular Sciences (Online)
- Additional Journal Information:
- Journal Name: International Journal of Molecular Sciences (Online); Journal Volume: 20; Journal Issue: 19; Journal ID: ISSN 1422-0067
- Publisher:
- MDPI
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; Biochemistry & Molecular Biology; Chemistry
Citation Formats
Hwang, Sungmin, Chavarria, Nikita, Hackley, Rylee, Schmid, Amy, and Maupin-Furlow, Julie. Gene Expression of Haloferax volcanii on Intermediate and Abundant Sources of Fixed Nitrogen. United States: N. p., 2019.
Web. doi:10.3390/ijms20194784.
Hwang, Sungmin, Chavarria, Nikita, Hackley, Rylee, Schmid, Amy, & Maupin-Furlow, Julie. Gene Expression of Haloferax volcanii on Intermediate and Abundant Sources of Fixed Nitrogen. United States. https://doi.org/10.3390/ijms20194784
Hwang, Sungmin, Chavarria, Nikita, Hackley, Rylee, Schmid, Amy, and Maupin-Furlow, Julie. Thu .
"Gene Expression of Haloferax volcanii on Intermediate and Abundant Sources of Fixed Nitrogen". United States. https://doi.org/10.3390/ijms20194784. https://www.osti.gov/servlets/purl/1800240.
@article{osti_1800240,
title = {Gene Expression of Haloferax volcanii on Intermediate and Abundant Sources of Fixed Nitrogen},
author = {Hwang, Sungmin and Chavarria, Nikita and Hackley, Rylee and Schmid, Amy and Maupin-Furlow, Julie},
abstractNote = {Haloferax volcanii, a well-developed model archaeon for genomic, transcriptomic, and proteomic analyses, can grow on a defined medium of abundant and intermediate levels of fixed nitrogen. Here we report a global profiling of gene expression of H. volcanii grown on ammonium as an abundant source of fixed nitrogen compared to l-alanine, the latter of which exemplifies an intermediate source of nitrogen that can be obtained from dead cells in natural habitats. By comparing the two growth conditions, 30 genes were found to be differentially expressed, including 16 genes associated with amino acid metabolism and transport. The gene expression profiles contributed to mapping ammonium and l-alanine usage with respect to transporters and metabolic pathways. In addition, conserved DNA motifs were identified in the putative promoter regions and transcription factors were found to be in synteny with the differentially expressed genes, leading us to propose regulons of transcriptionally co-regulated operons. This study provides insight to how H. volcanii responds to and utilizes intermediate vs. abundant sources of fixed nitrogen for growth, with implications for conserved functions in related halophilic archaea.},
doi = {10.3390/ijms20194784},
journal = {International Journal of Molecular Sciences (Online)},
number = 19,
volume = 20,
place = {United States},
year = {Thu Sep 26 00:00:00 EDT 2019},
month = {Thu Sep 26 00:00:00 EDT 2019}
}
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