Pectin methylesterase selectively softens the onion epidermal wall yet reduces acid-induced creep
Abstract
De-esterification of homogalacturonan (HG) is thought to stiffen pectin gels and primary cell walls by increasing calcium cross-linking between HG chains. Contrary to this idea, recent studies found that HG de-esterification correlated with reduced stiffness of living tissues, measured by surface indentation. The physical basis of such apparent wall softening is unclear, but possibly involves complex biological responses to HG modification. To assess the direct physical consequences of HG de-esterification on wall mechanics without such complications, we treated isolated onion (Allium cepa) epidermal walls with pectin methylesterase (PME) and assessed wall biomechanics with indentation and tensile tests. In nanoindentation assays, PME action softened the wall (reduced the indentation modulus). In tensile force/extension assays, PME increased plasticity, but not elasticity. These softening effects are attributed, at least in part, to increased electrostatic repulsion and swelling of the wall after PME treatment. Despite softening and swelling upon HG de-esterification, PME treatment alone failed to induce cell wall creep. Instead, acid-induced creep, mediated by endogenous α-expansin, was reduced. We conclude that HG de-esterification physically softens the onion wall, yet reduces expansin-mediated wall extensibility.
- Publication Date:
- Research Org.:
- Energy Frontier Research Centers (EFRC) (United States). Center for Lignocellulose Structure and Formation (CLSF); Pennsylvania State Univ., University Park, PA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1767734
- Grant/Contract Number:
- SC0001090
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Experimental Botany
- Additional Journal Information:
- Journal Volume: 71; Journal Issue: 9; Journal ID: ISSN 0022-0957
- Publisher:
- Oxford University Press
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; biofuels (including algae and biomass); bio-inspired; membrane; carbon sequestration; materials and chemistry by design; synthesis (self-assembly); atomic force microscopy; biomechanics; expansin; homogalacturonan; nanoindentation; onion (Allium cepa) epidermis; pectin methylesterase; plant cell wall mechanics; tensile testing; wall hydration
Citation Formats
Wang, Xuan, Wilson, Liza, and Cosgrove, Daniel J. Pectin methylesterase selectively softens the onion epidermal wall yet reduces acid-induced creep. United States: N. p., 2020.
Web. doi:10.1093/jxb/eraa059.
Wang, Xuan, Wilson, Liza, & Cosgrove, Daniel J. Pectin methylesterase selectively softens the onion epidermal wall yet reduces acid-induced creep. United States. https://doi.org/10.1093/jxb/eraa059
Wang, Xuan, Wilson, Liza, and Cosgrove, Daniel J. Sat .
"Pectin methylesterase selectively softens the onion epidermal wall yet reduces acid-induced creep". United States. https://doi.org/10.1093/jxb/eraa059. https://www.osti.gov/servlets/purl/1767734.
@article{osti_1767734,
title = {Pectin methylesterase selectively softens the onion epidermal wall yet reduces acid-induced creep},
author = {Wang, Xuan and Wilson, Liza and Cosgrove, Daniel J.},
abstractNote = {De-esterification of homogalacturonan (HG) is thought to stiffen pectin gels and primary cell walls by increasing calcium cross-linking between HG chains. Contrary to this idea, recent studies found that HG de-esterification correlated with reduced stiffness of living tissues, measured by surface indentation. The physical basis of such apparent wall softening is unclear, but possibly involves complex biological responses to HG modification. To assess the direct physical consequences of HG de-esterification on wall mechanics without such complications, we treated isolated onion (Allium cepa) epidermal walls with pectin methylesterase (PME) and assessed wall biomechanics with indentation and tensile tests. In nanoindentation assays, PME action softened the wall (reduced the indentation modulus). In tensile force/extension assays, PME increased plasticity, but not elasticity. These softening effects are attributed, at least in part, to increased electrostatic repulsion and swelling of the wall after PME treatment. Despite softening and swelling upon HG de-esterification, PME treatment alone failed to induce cell wall creep. Instead, acid-induced creep, mediated by endogenous α-expansin, was reduced. We conclude that HG de-esterification physically softens the onion wall, yet reduces expansin-mediated wall extensibility.},
doi = {10.1093/jxb/eraa059},
journal = {Journal of Experimental Botany},
number = 9,
volume = 71,
place = {United States},
year = {Sat Feb 01 00:00:00 EST 2020},
month = {Sat Feb 01 00:00:00 EST 2020}
}
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