Nuclear and chloroplast genome engineering of a productive non-model alga Desmodesmus armatus: Insights into unusual and selective acquisition mechanisms for foreign DNA
Abstract
Despite the tremendous potential of algae to contribute to a future bioeconomy, there are practical and theoretical limitations to how well naturally sourced species and strains can perform in an outdoor setting. The application of biotechnology to modulate and engineer algae metabolism or to increase performance, resilience, or produce novel compounds, offers opportunities to overcome some of the major commercialization barriers. There are numerous approaches reported in the literature having variable success on genetic engineering of algae with non-model algae often presenting unique challenges to genetic engineering. We report here on successful nuclear and chloroplast genomic integration of selection marker resistance in the non-model alga Desmodesmus armatus. Nuclear transformation was accomplished using both electroporation and Agrobacterium-mediated approaches. However, in all surviving transformants, DNA integration was accompanied by excision and/or rearrangement of the gene of interest and fluorescence reporter coding sequences. Similarly, chloroplast transformation was successfully accomplished using a biolistic DNA delivery method. For these transformants, we also observed off-target mutations in the chloroplast genome, not previously observed in other, more routinely used, algae species. Finally, we present insights into potential mechanisms for these observed truncations, rearrangements, and mutations in D. armatus.
- Authors:
-
[1];
[1];
[1]
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Colorado School of Mines, Golden, CO (United States)
- Arizona State Univ., Mesa, AZ (United States)
- Publication Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office
- OSTI Identifier:
- 1764916
- Alternate Identifier(s):
- OSTI ID: 1809807
- Report Number(s):
- NREL-JA-2700-77862
Journal ID: ISSN 2211-9264; MainId:30777;UUID:0b1ece19-5ecc-472c-b12b-e0250934f512;MainAdminID:19266
- Grant/Contract Number:
- AC36-08GO28308
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Algal Research
- Additional Journal Information:
- Journal Volume: 53; Journal ID: ISSN 2211-9264
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Algae; Biorefinery; Genetic engineering; Transformation; Biolistics; Transcriptomics
Citation Formats
Douchi, Damien, Mosey, Megan, Astling, David P., Knoshaug, Eric P., Nag, Ambarish, McGowen, John, and Laurens, Lieve M. L. Nuclear and chloroplast genome engineering of a productive non-model alga Desmodesmus armatus: Insights into unusual and selective acquisition mechanisms for foreign DNA. United States: N. p., 2021.
Web. doi:10.1016/j.algal.2020.102152.
Douchi, Damien, Mosey, Megan, Astling, David P., Knoshaug, Eric P., Nag, Ambarish, McGowen, John, & Laurens, Lieve M. L. Nuclear and chloroplast genome engineering of a productive non-model alga Desmodesmus armatus: Insights into unusual and selective acquisition mechanisms for foreign DNA. United States. https://doi.org/10.1016/j.algal.2020.102152
Douchi, Damien, Mosey, Megan, Astling, David P., Knoshaug, Eric P., Nag, Ambarish, McGowen, John, and Laurens, Lieve M. L. Mon .
"Nuclear and chloroplast genome engineering of a productive non-model alga Desmodesmus armatus: Insights into unusual and selective acquisition mechanisms for foreign DNA". United States. https://doi.org/10.1016/j.algal.2020.102152. https://www.osti.gov/servlets/purl/1764916.
@article{osti_1764916,
title = {Nuclear and chloroplast genome engineering of a productive non-model alga Desmodesmus armatus: Insights into unusual and selective acquisition mechanisms for foreign DNA},
author = {Douchi, Damien and Mosey, Megan and Astling, David P. and Knoshaug, Eric P. and Nag, Ambarish and McGowen, John and Laurens, Lieve M. L.},
abstractNote = {Despite the tremendous potential of algae to contribute to a future bioeconomy, there are practical and theoretical limitations to how well naturally sourced species and strains can perform in an outdoor setting. The application of biotechnology to modulate and engineer algae metabolism or to increase performance, resilience, or produce novel compounds, offers opportunities to overcome some of the major commercialization barriers. There are numerous approaches reported in the literature having variable success on genetic engineering of algae with non-model algae often presenting unique challenges to genetic engineering. We report here on successful nuclear and chloroplast genomic integration of selection marker resistance in the non-model alga Desmodesmus armatus. Nuclear transformation was accomplished using both electroporation and Agrobacterium-mediated approaches. However, in all surviving transformants, DNA integration was accompanied by excision and/or rearrangement of the gene of interest and fluorescence reporter coding sequences. Similarly, chloroplast transformation was successfully accomplished using a biolistic DNA delivery method. For these transformants, we also observed off-target mutations in the chloroplast genome, not previously observed in other, more routinely used, algae species. Finally, we present insights into potential mechanisms for these observed truncations, rearrangements, and mutations in D. armatus.},
doi = {10.1016/j.algal.2020.102152},
journal = {Algal Research},
number = ,
volume = 53,
place = {United States},
year = {Mon Mar 01 00:00:00 EST 2021},
month = {Mon Mar 01 00:00:00 EST 2021}
}
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Works referenced in this record:
Conservation and divergence in eukaryotic DNA methylation
journal, May 2010
- Lee, T. -f.; Zhai, J.; Meyers, B. C.
- Proceedings of the National Academy of Sciences, Vol. 107, Issue 20
High-Throughput Genotyping of Green Algal Mutants Reveals Random Distribution of Mutagenic Insertion Sites and Endonucleolytic Cleavage of Transforming DNA
journal, April 2014
- Zhang, Ru; Patena, Weronika; Armbruster, Ute
- The Plant Cell, Vol. 26, Issue 4
The Chlamydomonas Chloroplast HLP Protein Is Required for Nucleoid Organization and Genome Maintenance
journal, November 2009
- Karcher, Daniel; Köster, Dietrich; Schadach, Anne
- Molecular Plant, Vol. 2, Issue 6
Transformation of the green alga Chlamydomonas reinhardii with yeast DNA
journal, March 1982
- Rochaix, J. -D.; van Dillewijn, J.
- Nature, Vol. 296, Issue 5852
Structure-based design of novel Chlamydomonas reinhardtii D1-D2 photosynthetic proteins for herbicide monitoring
journal, October 2009
- Rea, Giuseppina; Polticelli, Fabio; Antonacci, Amina
- Protein Science, Vol. 18, Issue 10
CO2 capture from air by Chlorella vulgaris microalgae in an airlift photobioreactor
journal, November 2017
- Sadeghizadeh, Aziz; Farhad dad, Farid; Moghaddasi, Leila
- Bioresource Technology, Vol. 243
Placing microalgae on the biofuels priority list: a review of the technological challenges
journal, November 2009
- Greenwell, H. C.; Laurens, L. M. L.; Shields, R. J.
- Journal of The Royal Society Interface, Vol. 7, Issue 46
A link between DNA methylation and epigenetic silencing in transgenic Volvox carteri
journal, March 2001
- Babinger, P.
- Nucleic Acids Research, Vol. 29, Issue 6
Algal Proteins: Extraction, Application, and Challenges Concerning Production
journal, April 2017
- Bleakley, Stephen; Hayes, Maria
- Foods, Vol. 6, Issue 5
Micro and macroalgal biomass: A renewable source for bioethanol
journal, January 2011
- John, Rojan P.; Anisha, G. S.; Nampoothiri, K. Madhavan
- Bioresource Technology, Vol. 102, Issue 1
Determination of Total Solids and Ash in Algal Biomass: Laboratory Analytical Procedure (LAP)
report, January 2016
- Van Wychen, Stefanie; Laurens, Lieve M. L.
Direct capture and conversion of CO 2 from air by growing a cyanobacterial consortium at pH up to 11.2
journal, April 2019
- Ataeian, Maryam; Liu, Yihua; Canon‐Rubio, Karen Andrea
- Biotechnology and Bioengineering, Vol. 116, Issue 7
The Algae Testbed Public-Private Partnership (ATP3) framework; establishment of a national network of testbed sites to support sustainable algae production
journal, July 2017
- McGowen, John; Knoshaug, Eric P.; Laurens, Lieve M. L.
- Algal Research, Vol. 25
An mt+ gamete-specific nuclease that targets mt- chloroplasts during sexual reproduction in C. reinhardtii
journal, May 2002
- Nishimura, Y.
- Genes & Development, Vol. 16, Issue 9
Establishment of an efficient genetic transformation system in Scenedesmus obliquus
journal, January 2013
- Guo, Suo-Lian; Zhao, Xin-Qing; Tang, Ying
- Journal of Biotechnology, Vol. 163, Issue 1
Development of an electrotransformation protocol for genetic manipulation of Clostridium pasteurianum
journal, January 2013
- Pyne, Michael E.; Moo-Young, Murray; Chung, Duane A.
- Biotechnology for Biofuels, Vol. 6, Issue 1
Mg 2+ -free buffer elevates transformation efficiency of Vibrio parahaemolyticus by electroporation
journal, March 2009
- Wang, H.; Griffiths, M. W.
- Letters in Applied Microbiology, Vol. 48, Issue 3
Development of a high-productivity, halophilic, thermotolerant microalga Picochlorum renovo
journal, October 2019
- Dahlin, Lukas R.; Gerritsen, Alida T.; Henard, Calvin A.
- Communications Biology, Vol. 2, Issue 1
Stable Nuclear Transformation System for the Coccolithophorid Alga Pleurochrysis carterae
journal, March 2016
- Endo, Hirotoshi; Yoshida, Megumi; Uji, Toshiki
- Scientific Reports, Vol. 6, Issue 1
Structure-guided Analysis Reveals Nine Sequence Motifs Conserved among DNA Amino-methyl-transferases, and Suggests a Catalytic Mechanism for these Enzymes
journal, November 1995
- Malone, Thomas; Blumenthal, Robert M.; Cheng, Xiaodong
- Journal of Molecular Biology, Vol. 253, Issue 4
A rapid and simple method for detection of type II restriction endonucleases in cells of bacteria with high activity of nonspecific nucleases.
journal, November 2012
- Podgórska, Beata; Kujawska, Grażyna; Skurzewski, Michał
- Acta Biochimica Polonica, Vol. 59, Issue 4
The HSP70A promoter as a tool for the improved expression of transgenes in Chlamydomonas
journal, January 2000
- Schroda, Michael; Blocker, Dagmar; Beck, Christoph F.
- The Plant Journal, Vol. 21, Issue 2
Bio-functionalities of proteins derived from marine algae — A review
journal, October 2012
- Samarakoon, Kalpa; Jeon, You-Jin
- Food Research International, Vol. 48, Issue 2
Transformation of yeast spheroplasts without cell fusion
journal, June 1987
- Burgers, Peter M. J.; Percival, Kimberly J.
- Analytical Biochemistry, Vol. 163, Issue 2
Enhanced Transformation Efficiency of Recalcitrant Bacillus cereus and Bacillus weihenstephanensis Isolates upon In Vitro Methylation of Plasmid DNA
journal, October 2008
- Groot, Masja Nierop; Nieboer, Frank; Abee, Tjakko
- Applied and Environmental Microbiology, Vol. 74, Issue 24
Fatty Amide Determination in Neutral Molecular Fractions of Green Crude Hydrothermal Liquefaction Oils From Algal Biomass
journal, July 2017
- Palardy, Oliver; Behnke, Craig; Laurens, Lieve M. L.
- Energy & Fuels, Vol. 31, Issue 8
Strategies to Combat Antibiotic Resistance in the Wastewater Treatment Plants
journal, January 2018
- Barancheshme, Fateme; Munir, Mariya
- Frontiers in Microbiology, Vol. 8
Insensitivity of chloroplast gene expression to DNA methylation
journal, March 2009
- Ahlert, Daniela; Stegemann, Sandra; Kahlau, Sabine
- Molecular Genetics and Genomics, Vol. 282, Issue 1
Algal Bioremediation of Waste Waters from Land-Based Aquaculture Using Ulva: Selecting Target Species and Strains
journal, October 2013
- Lawton, Rebecca J.; Mata, Leonardo; de Nys, Rocky
- PLoS ONE, Vol. 8, Issue 10
Impacts of nitrogen and phosphorus starvation on the physiology of Chlamydomonas reinhardtii
journal, October 2015
- Kamalanathan, Manoj; Pierangelini, Mattia; Shearman, Lauren Ann
- Journal of Applied Phycology, Vol. 28, Issue 3
A robust protocol for efficient generation, and genomic characterization of insertional mutants of Chlamydomonas reinhardtii
journal, April 2017
- Pollock, Steve V.; Mukherjee, Bratati; Bajsa-Hirschel, Joanna
- Plant Methods, Vol. 13, Issue 1
Enzymatic cell wall degradation of Chlorella vulgaris and other microalgae for biofuels production
journal, September 2012
- Gerken, Henri G.; Donohoe, Bryon; Knoshaug, Eric P.
- Planta, Vol. 237, Issue 1
Iterative screening of an evolutionary engineered Desmodesmus generates robust field strains with pesticide tolerance
journal, April 2018
- Corcoran, Alina A.; Saunders, Matthew A.; Hanley, Adam P.
- Algal Research, Vol. 31
Cloning and Characterization of a Periplasmic Nuclease of Vibrio vulnificus and Its Role in Preventing Uptake of Foreign DNA
journal, January 2001
- Wu, Shi-I; Lo, Shi-Kan; Shao, Chung-Ping
- Applied and Environmental Microbiology, Vol. 67, Issue 1
Evaluation of secretion reporters to microalgae biotechnology: Blue to red fluorescent proteins
journal, April 2018
- Molino, João Vitor Dutra; de Carvalho, João Carlos Monteiro; Mayfield, Stephen
- Algal Research, Vol. 31
Stable transformation of the green algae Acutodesmus obliquus and Neochloris oleoabundans based on E. coli conjugation
journal, May 2019
- Muñoz, Camilo F.; Sturme, Mark H. J.; D'Adamo, Sarah
- Algal Research, Vol. 39
MultiMotifMaker: A Multi-Thread Tool for Identifying DNA Methylation Motifs from Pacbio Reads
journal, January 2020
- Li, Tao; Zhang, Xiankai; Luo, Feng
- IEEE/ACM Transactions on Computational Biology and Bioinformatics, Vol. 17, Issue 1
High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp.
journal, November 2011
- Kilian, O.; Benemann, C. S. E.; Niyogi, K. K.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 52, p. 21265-21269
UV-mediated Chlamydomonas mutants with enhanced nuclear transgene expression by disruption of DNA methylation-dependent and independent silencing systems
journal, October 2016
- Kurniasih, Sari Dewi; Yamasaki, Tomohito; Kong, Fantao
- Plant Molecular Biology, Vol. 92, Issue 6
Hydrothermal liquefaction of biomass: Developments from batch to continuous process
journal, February 2015
- Elliott, Douglas C.; Biller, Patrick; Ross, Andrew B.
- Bioresource Technology, Vol. 178, p. 147-156
N6-Methyldeoxyadenosine Marks Active Transcription Start Sites in Chlamydomonas
journal, May 2015
- Fu, Ye; Luo, Guan-Zheng; Chen, Kai
- Cell, Vol. 161, Issue 4
Microalgae cultivation for biosurfactant production [English]
journal, November 2015
- Elis acirc ngela, Martha Radmann; Etiele, Greque de Morais; Cibele, Freitas de Oliveira
- African Journal of Microbiology Research, Vol. 9, Issue 47
SortMeRNA: fast and accurate filtering of ribosomal RNAs in metatranscriptomic data
journal, October 2012
- Kopylova, Evguenia; Noé, Laurent; Touzet, Hélène
- Bioinformatics, Vol. 28, Issue 24
Role of Chloroplast Protein Kinase Stt7 in LHCII Phosphorylation and State Transition in Chlamydomonas
journal, March 2003
- Depege, N.
- Science, Vol. 299, Issue 5612
Is homologous recombination really an error-free process?
journal, June 2014
- Guirouilh-Barbat, Josée; Lambert, Sarah; Bertrand, Pascale
- Frontiers in Genetics, Vol. 5
Effects of overexpressing photosynthetic carbon flux control enzymes in the cyanobacterium Synechocystis PCC 6803
journal, November 2016
- Liang, Feiyan; Lindblad, Peter
- Metabolic Engineering, Vol. 38
Microalgae as biodiesel & biomass feedstocks: Review & analysis of the biochemistry, energetics & economics
journal, January 2010
- Williams, Peter J. le B.; Laurens, Lieve M. L.
- Energy & Environmental Science, Vol. 3, Issue 5
The argininosuccinate lyase gene of Chlamydomonas reinhardtii: an important tool for nuclear transformation and for correlating the genetic and molecular maps of the ARG7 locus.
journal, October 1989
- Debuchy, R.; Purton, S.; Rochaix, J. D.
- The EMBO Journal, Vol. 8, Issue 10
Polyols and Polyurethanes from Crude Algal Oil
journal, April 2013
- Petrović, Zoran S.; Wan, Xianmei; Bilić, Olivera
- Journal of the American Oil Chemists' Society, Vol. 90, Issue 7
Thermal Treatment of Crude Algae Oils Prepared Under Hydrothermal Extraction Conditions
journal, July 2012
- Roussis, Stilianos G.; Cranford, Richard; Sytkovetskiy, Naum
- Energy & Fuels, Vol. 26, Issue 8
Agrobacterium-mediated and electroporation-mediated transformation of Chlamydomonas reinhardtii: a comparative study
journal, February 2018
- Mini, Paola; Demurtas, Olivia Costantina; Valentini, Silvia
- BMC Biotechnology, Vol. 18, Issue 1
Photosystem-II D1 protein mutants of Chlamydomonas reinhardtii in relation to metabolic rewiring and remodelling of H-bond network at QB site
journal, October 2018
- Antonacci, Amina; Lambreva, Maya D.; Margonelli, Andrea
- Scientific Reports, Vol. 8, Issue 1
In Vitro CpG Methylation Increases the Transformation Efficiency of Borrelia burgdorferi Strains Harboring the Endogenous Linear Plasmid lp56
journal, October 2008
- Chen, Q.; Fischer, J. R.; Benoit, V. M.
- Journal of Bacteriology, Vol. 190, Issue 24
Optimization of an electroporation protocol using the K562 cell line as a model: role of cell cycle phase and cytoplasmic DNAses
journal, November 2006
- Delgado-Cañedo, Andrés; Santos, Daniel Garcia dos; Chies, José Artur Bogo
- Cytotechnology, Vol. 51, Issue 3
Evaluation of strategies for improving the transgene expression in an oleaginous microalga Scenedesmus acutus
journal, January 2019
- Suttangkakul, Anongpat; Sirikhachornkit, Anchalee; Juntawong, Piyada
- BMC Biotechnology, Vol. 19, Issue 1
Harmonization of experimental approach and data collection to streamline analysis of biomass composition from algae in an inter-laboratory setting
journal, July 2017
- Laurens, Lieve M. L.; Van Wychen, Stefanie; Pienkos, Philip T.
- Algal Research, Vol. 25
Insights into the role of DNA methylation in diatoms by genome-wide profiling in Phaeodactylum tricornutum
journal, July 2013
- Veluchamy, Alaguraj; Lin, Xin; Maumus, Florian
- Nature Communications, Vol. 4, Issue 1
A new arabinomannan from the cell wall of the chlorococcal algae Chlorella vulgaris
journal, May 2012
- Pieper, Susanne; Unterieser, Inga; Mann, Florian
- Carbohydrate Research, Vol. 352
Nourseothricin N-acetyl transferase (NAT), a new selectable marker for nuclear gene expression in Chlamydomonas
journal, November 2019
- Yang, Xinjia; Peng, Jialin; Pan, Junmin
- Plant Methods, Vol. 15, Issue 1
Two thermostable nucleases coexisted in Staphylococcus aureus : evidence from mutagenesis and in vitro expression
journal, July 2008
- Tang, Junni; Zhou, Rui; Shi, Xianming
- FEMS Microbiology Letters, Vol. 284, Issue 2
Kinetic and catalytic mechanism of HhaI methyltransferase.
journal, April 1987
- Wu, J. C.; Santi, D. V.
- Journal of Biological Chemistry, Vol. 262, Issue 10
Chloroplast transformation in Chlamydomonas with high velocity microprojectiles
journal, June 1988
- Boynton, J.; Gillham, N.; Harris, E.
- Science, Vol. 240, Issue 4858
Characterization of DNA methyltransferase specificities using single-molecule, real-time DNA sequencing
journal, December 2011
- Clark, Tyson A.; Murray, Iain A.; Morgan, Richard D.
- Nucleic Acids Research, Vol. 40, Issue 4
Synechocystis PCC 6803 overexpressing RuBisCO grow faster with increased photosynthesis
journal, June 2017
- Liang, Feiyan; Lindblad, Peter
- Metabolic Engineering Communications, Vol. 4
Inhibition of DNA Methylation in Picochlorum soloecismus Alters Algae Productivity
journal, October 2020
- Steadman, Christina R.; Banerjee, Shounak; Kunde, Yuliya A.
- Frontiers in Genetics, Vol. 11
Improved DNA/protein delivery in microalgae – A simple and reliable method for the prediction of optimal electroporation settings
journal, July 2018
- Muñoz, Camilo F.; de Jaeger, Lenny; Sturme, Mark H. J.
- Algal Research, Vol. 33
Histochemical GUS expression of beta tubulin promoter in transgenic tobacco [English]
journal, April 2017
- Hira, Mubeen; Aftab, Bashir; Ayesha, Ameen
- African Journal of Biotechnology, Vol. 16, Issue 17
Chloroplast DNA base substitutions: an experimental assessment
journal, March 2005
- GuhaMajumdar, Monica; Sears, Barbara B.
- Molecular Genetics and Genomics, Vol. 273, Issue 2
Acquired Antibiotic Resistance Genes: An Overview
journal, January 2011
- van Hoek, Angela H. A. M.; Mevius, Dik; Guerra, Beatriz
- Frontiers in Microbiology, Vol. 2
Membrane-Inlet Mass Spectrometry Enables a Quantitative Understanding of Inorganic Carbon Uptake Flux and Carbon Concentrating Mechanisms in Metabolically Engineered Cyanobacteria
journal, June 2019
- Douchi, Damien; Liang, Feiyan; Cano, Melissa
- Frontiers in Microbiology, Vol. 10
Long-Term Cultivation of Algae in Open-Raceway Ponds: Lessons from the Field
journal, August 2015
- White, Rebecca L.; Ryan, Rebecca A.
- Industrial Biotechnology, Vol. 11, Issue 4
Robust Expression and Secretion of Xylanase1 in Chlamydomonas reinhardtii by Fusion to a Selection Gene and Processing with the FMDV 2A Peptide
journal, August 2012
- Rasala, Beth A.; Lee, Philip A.; Shen, Zhouxin
- PLoS ONE, Vol. 7, Issue 8
The DNA (cytosine-5) methyltransferases
journal, January 1994
- Kumar, Sanjay; Cheng, Xiaodong; Klimasauskas, Saulius
- Nucleic Acids Research, Vol. 22, Issue 1
Coupling of Algal Biofuel Production with Wastewater
journal, January 2014
- Bhatt, Neha Chamoli; Panwar, Amit; Bisht, Tara Singh
- The Scientific World Journal, Vol. 2014
Separation and quantification of microalgal carbohydrates
journal, December 2012
- Templeton, David W.; Quinn, Matthew; Van Wychen, Stefanie
- Journal of Chromatography A, Vol. 1270
The induction and characterisation of cell wall mutants of Chlamydomonas reinhardi
journal, April 1972
- Hyams, Jerry; Davies, D. Roy
- Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 14, Issue 4
CaNAT1 , a Heterologous Dominant Selectable Marker for Transformation of Candida albicans and Other Pathogenic Candida Species
journal, February 2005
- Shen, Junqing; Guo, Weihui; Köhler, Julia R.
- Infection and Immunity, Vol. 73, Issue 2
Transcript-level expression analysis of RNA-seq experiments with HISAT, StringTie and Ballgown
journal, August 2016
- Pertea, Mihaela; Kim, Daehwan; Pertea, Geo M.
- Nature Protocols, Vol. 11, Issue 9