Transporters and ascorbate–glutathione metabolism for differential cadmium accumulation and tolerance in two contrasting willow genotypes
Abstract
Salix matsudana Koidz is a low cadmium (Cd)-accumulating willow, whereas its cultivated variety, Salix matsudana var. matsudana f. umbraculifera Rehd., is a high Cd-accumulating and tolerant willow (HCW). The physiological and molecular mechanisms underlying differential Cd accumulation and tolerance in the two Salix species are poorly understood. In this study, we confirmed that the differential Cd translocation capacity from roots to the shoots leads to the differential Cd accumulation in their aboveground parts between these two willow genotypes. Cadmium accumulation happens preferentially in the transport pathway, and Cd is mainly located in the vacuolar, cell wall and intercellular space in HCW bark by cadmium location analysis at tissue and subcellular levels. Comparative transcriptome analysis revealed that higher expressions of several metal transporter genes (ATP-binding cassette transporters, K+ transporters/channels, yellow stripe-like proteins, zinc-regulated transporter/iron-regulated transporter-like proteins, etc.) are involved in root uptake and translocation capacity in HCW; meanwhile, ascorbate–glutathione metabolic pathways play essential roles in Cd detoxification and higher tolerance of the Cd-accumulator HCW. These results lay the foundation for further understanding the molecular mechanisms of Cd accumulation in woody plants and provide new insights into molecular-assisted-screening woody plant varieties for phytoremediation.
- Authors:
-
[3];
- Chinese Academy of Forestry, Beijing (China)
- Chinese Academy of Forestry, Beijing (China); Henan Polytechnic Univ. (China)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE; National Natural Science Foundation of China (NSFC); Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding; Chinese Academy of Forestry; National Transformation Science and Technology Program; Key Technologies R & D Program of Henan Province
- OSTI Identifier:
- 1761667
- Grant/Contract Number:
- AC05-00OR22725; 31872168; 2016C02056-1; CAFYBB2017ZY007; 2018ZX08020002-005-003; 182102310052
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Tree Physiology (Online)
- Additional Journal Information:
- Journal Name: Tree Physiology (Online); Journal Volume: 40; Journal Issue: 8; Journal ID: ISSN 1758-4469
- Publisher:
- Oxford University Press
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Salix matsudana var. matsudana f. umbraculifera umbraculifera; cadmium stress; accumulation and detoxification; comparative morphophysiology and transcriptome; AsA–GSH metabolism pathway
Citation Formats
Han, Xiaojiao, Zhang, Yunxing, Yu, Miao, Zhang, Jin, Xu, Dong, Lu, Zhuchou, Qiao, Guirong, Qiu, Wenmin, and Zhuo, Renying. Transporters and ascorbate–glutathione metabolism for differential cadmium accumulation and tolerance in two contrasting willow genotypes. United States: N. p., 2020.
Web. doi:10.1093/treephys/tpaa029.
Han, Xiaojiao, Zhang, Yunxing, Yu, Miao, Zhang, Jin, Xu, Dong, Lu, Zhuchou, Qiao, Guirong, Qiu, Wenmin, & Zhuo, Renying. Transporters and ascorbate–glutathione metabolism for differential cadmium accumulation and tolerance in two contrasting willow genotypes. United States. https://doi.org/10.1093/treephys/tpaa029
Han, Xiaojiao, Zhang, Yunxing, Yu, Miao, Zhang, Jin, Xu, Dong, Lu, Zhuchou, Qiao, Guirong, Qiu, Wenmin, and Zhuo, Renying. Mon .
"Transporters and ascorbate–glutathione metabolism for differential cadmium accumulation and tolerance in two contrasting willow genotypes". United States. https://doi.org/10.1093/treephys/tpaa029. https://www.osti.gov/servlets/purl/1761667.
@article{osti_1761667,
title = {Transporters and ascorbate–glutathione metabolism for differential cadmium accumulation and tolerance in two contrasting willow genotypes},
author = {Han, Xiaojiao and Zhang, Yunxing and Yu, Miao and Zhang, Jin and Xu, Dong and Lu, Zhuchou and Qiao, Guirong and Qiu, Wenmin and Zhuo, Renying},
abstractNote = {Salix matsudana Koidz is a low cadmium (Cd)-accumulating willow, whereas its cultivated variety, Salix matsudana var. matsudana f. umbraculifera Rehd., is a high Cd-accumulating and tolerant willow (HCW). The physiological and molecular mechanisms underlying differential Cd accumulation and tolerance in the two Salix species are poorly understood. In this study, we confirmed that the differential Cd translocation capacity from roots to the shoots leads to the differential Cd accumulation in their aboveground parts between these two willow genotypes. Cadmium accumulation happens preferentially in the transport pathway, and Cd is mainly located in the vacuolar, cell wall and intercellular space in HCW bark by cadmium location analysis at tissue and subcellular levels. Comparative transcriptome analysis revealed that higher expressions of several metal transporter genes (ATP-binding cassette transporters, K+ transporters/channels, yellow stripe-like proteins, zinc-regulated transporter/iron-regulated transporter-like proteins, etc.) are involved in root uptake and translocation capacity in HCW; meanwhile, ascorbate–glutathione metabolic pathways play essential roles in Cd detoxification and higher tolerance of the Cd-accumulator HCW. These results lay the foundation for further understanding the molecular mechanisms of Cd accumulation in woody plants and provide new insights into molecular-assisted-screening woody plant varieties for phytoremediation.},
doi = {10.1093/treephys/tpaa029},
journal = {Tree Physiology (Online)},
number = 8,
volume = 40,
place = {United States},
year = {Mon Mar 16 00:00:00 EDT 2020},
month = {Mon Mar 16 00:00:00 EDT 2020}
}
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