Recycling of Nutrients from Dairy Wastewater by Extremophilic Microalgae with High Ammonia Tolerance
Abstract
This study explored the possibility of incorporating extremophilic algal cultivation into dairy wastewater treatment by characterizing a unique algal strain. Results showed that extremophilic microalgae Chlorella vulgaris CA1 newly isolated from dairy wastewater tolerated a high level of ammonia nitrogen (2.7 g/L), which was over 20 times the ammonia nitrogen that regular Chlorella sp. could tolerate. The isolate was mixotrophically cultured in dairy effluent treated by anaerobic digestion (AD) for recycling nutrients and polishing the wastewater. The highest biomass content of 13.3 g/L and protein content of 43.4% were achieved in the culture in AD effluent. Up to 96% of the total nitrogen and 79% of the total phosphorus were removed from the dairy AD effluent. The ability of the algae to tolerate a high level of ammonia nitrogen suggests the potential for direct nutrient recycling from dairy wastewater while producing algal biomass and high value bioproducts.
- Authors:
-
[1];
[2];
- Washington State Univ., Pullman, WA (United States)
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Northwest A & F Univ., Shaanxi (China)
- Publication Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office (BETO)
- OSTI Identifier:
- 1755727
- Report Number(s):
- NREL/JA-2800-78276
Journal ID: ISSN 0013-936X; MainId:32193;UUID:39a7df61-863d-4f5c-9880-dac8c48a4774;MainAdminID:19052
- Grant/Contract Number:
- AC36-08GO28308
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Environmental Science and Technology
- Additional Journal Information:
- Journal Volume: 54; Journal Issue: 23; Journal ID: ISSN 0013-936X
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 54 ENVIRONMENTAL SCIENCES; Chlorella vulgaris CA1; dairy wastewater treatment; mixotrophic culture; ammonia nitrogen tolerance; protein-rich biomass; nutrient removal
Citation Formats
Pang, Na, Bergeron, Andre David, Gu, Xiangyu, Fu, Xiao, Dong, Tao, Yao, Yiqing, and Chen, Shulin. Recycling of Nutrients from Dairy Wastewater by Extremophilic Microalgae with High Ammonia Tolerance. United States: N. p., 2020.
Web. doi:10.1021/acs.est.0c02833.
Pang, Na, Bergeron, Andre David, Gu, Xiangyu, Fu, Xiao, Dong, Tao, Yao, Yiqing, & Chen, Shulin. Recycling of Nutrients from Dairy Wastewater by Extremophilic Microalgae with High Ammonia Tolerance. United States. https://doi.org/10.1021/acs.est.0c02833
Pang, Na, Bergeron, Andre David, Gu, Xiangyu, Fu, Xiao, Dong, Tao, Yao, Yiqing, and Chen, Shulin. Sun .
"Recycling of Nutrients from Dairy Wastewater by Extremophilic Microalgae with High Ammonia Tolerance". United States. https://doi.org/10.1021/acs.est.0c02833. https://www.osti.gov/servlets/purl/1755727.
@article{osti_1755727,
title = {Recycling of Nutrients from Dairy Wastewater by Extremophilic Microalgae with High Ammonia Tolerance},
author = {Pang, Na and Bergeron, Andre David and Gu, Xiangyu and Fu, Xiao and Dong, Tao and Yao, Yiqing and Chen, Shulin},
abstractNote = {This study explored the possibility of incorporating extremophilic algal cultivation into dairy wastewater treatment by characterizing a unique algal strain. Results showed that extremophilic microalgae Chlorella vulgaris CA1 newly isolated from dairy wastewater tolerated a high level of ammonia nitrogen (2.7 g/L), which was over 20 times the ammonia nitrogen that regular Chlorella sp. could tolerate. The isolate was mixotrophically cultured in dairy effluent treated by anaerobic digestion (AD) for recycling nutrients and polishing the wastewater. The highest biomass content of 13.3 g/L and protein content of 43.4% were achieved in the culture in AD effluent. Up to 96% of the total nitrogen and 79% of the total phosphorus were removed from the dairy AD effluent. The ability of the algae to tolerate a high level of ammonia nitrogen suggests the potential for direct nutrient recycling from dairy wastewater while producing algal biomass and high value bioproducts.},
doi = {10.1021/acs.est.0c02833},
journal = {Environmental Science and Technology},
number = 23,
volume = 54,
place = {United States},
year = {Sun Nov 15 00:00:00 EST 2020},
month = {Sun Nov 15 00:00:00 EST 2020}
}
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Works referenced in this record:
Growth of three microalgae strains and nutrient removal from an agro-zootechnical digestate
journal, July 2013
- Franchino, Marta; Comino, Elena; Bona, Francesca
- Chemosphere, Vol. 92, Issue 6
Effect of nitrogen source in low-cost media on biomass and lipid productivity of Nannochloropsis salina for large-scale biodiesel production: Sustainable Energy Section
journal, March 2014
- Liu, Junying; Bangert, Krys
- Environmental Progress & Sustainable Energy, Vol. 34, Issue 1
Invited review: Anaerobic fermentation of dairy food wastewater
journal, November 2012
- Hassan, A. N.; Nelson, B. K.
- Journal of Dairy Science, Vol. 95, Issue 11
Multilevel heuristic LED regime for stimulating lipid and bioproducts biosynthesis in Haematococcus pluvialis under mixotrophic conditions
journal, September 2019
- Pang, Na; Fu, Xiao; Fernandez, Jose S. Martinez
- Bioresource Technology, Vol. 288
Extremophilic micro-algae and their potential contribution in biotechnology
journal, May 2015
- Varshney, Prachi; Mikulic, Paulina; Vonshak, Avigad
- Bioresource Technology, Vol. 184
Solar biofuels production with microalgae
journal, October 2016
- Hallenbeck, P. C.; Grogger, M.; Mraz, M.
- Applied Energy, Vol. 179
Mixotrophic cultivation of a Chlorella sorokiniana strain for enhanced biomass and lipid production
journal, July 2014
- Li, Tingting; Zheng, Yubin; Yu, Liang
- Biomass and Bioenergy, Vol. 66
A review on the use of microalgal consortia for wastewater treatment
journal, June 2017
- Gonçalves, Ana L.; Pires, José C. M.; Simões, Manuel
- Algal Research, Vol. 24
Sequential cultivation of microalgae in raw and recycled dairy wastewater: Microalgal growth, wastewater treatment and biochemical composition
journal, February 2019
- Daneshvar, Ehsan; Zarrinmehr, Mohammad Javad; Koutra, Eleni
- Bioresource Technology, Vol. 273
Mini-review: A priori considerations for bacteria–algae interactions in algal biofuel systems receiving municipal wastewaters
journal, April 2014
- Unnithan, Veena V.; Unc, Adrian; Smith, Geoffrey B.
- Algal Research, Vol. 4
Nutrient removal and biodiesel production by integration of freshwater algae cultivation with piggery wastewater treatment
journal, September 2013
- Zhu, Liandong; Wang, Zhongming; Shu, Qing
- Water Research, Vol. 47, Issue 13
Harmful algal blooms and eutrophication: Nutrient sources, composition, and consequences
journal, August 2002
- Anderson, Donald M.; Glibert, Patricia M.; Burkholder, Joann M.
- Estuaries, Vol. 25, Issue 4
Anaerobic digested dairy manure as a nutrient supplement for cultivation of oil-rich green microalgae Chlorella sp.
journal, April 2010
- Wang, Liang; Li, Yecong; Chen, Paul
- Bioresource Technology, Vol. 101, Issue 8
Regulation and stimulation of photosynthesis of mixotrophically cultured Haematococcus pluvialis by ribose
journal, May 2019
- Pang, Na; Xie, Yuxiao; Oung, Hui Min Olivia
- Algal Research, Vol. 39
Cultivation of Chlorella vulgaris in manure-free piggery wastewater with high-strength ammonium for nutrients removal and biomass production: Effect of ammonium concentration, carbon/nitrogen ratio and pH
journal, February 2019
- Zheng, Hongli; Wu, Xiaodan; Zou, Guyue
- Bioresource Technology, Vol. 273
Achieving Stable Mainstream Nitrogen Removal via the Nitrite Pathway by Sludge Treatment Using Free Ammonia
journal, August 2017
- Wang, Qilin; Duan, Haoran; Wei, Wei
- Environmental Science & Technology, Vol. 51, Issue 17
Prospects, recent advancements and challenges of different wastewater streams for microalgal cultivation
journal, December 2017
- Guldhe, Abhishek; Kumari, Sheena; Ramanna, Luveshan
- Journal of Environmental Management, Vol. 203
Photolithotrophy, photoheterotrophy, and chemoheterotrophy: Patterns of resource utilization on an annual and a diurnal basis within a pelagic microbial community
journal, March 1979
- McKinley, Kelton R.; Wetzel, Robert G.
- Microbial Ecology, Vol. 5, Issue 1
Enhanced growth and lipid production of microalgae under mixotrophic culture condition: Effect of light intensity, glucose concentration and fed-batch cultivation
journal, April 2012
- Cheirsilp, Benjamas; Torpee, Salwa
- Bioresource Technology, Vol. 110
Acclimation and toxicity of high ammonium concentrations to unicellular algae
journal, March 2014
- Collos, Yves; Harrison, Paul J.
- Marine Pollution Bulletin, Vol. 80, Issue 1-2
Recent development of hydrothermal liquefaction for algal biorefinery
journal, April 2020
- Gu, X.; Martinez-Fernandez, J. S.; Pang, N.
- Renewable and Sustainable Energy Reviews, Vol. 121
Isolation, characterization, and validation of oleaginous, multi-trophic, and haloalkaline-tolerant microalgae for two-stage cultivation
journal, April 2014
- Wensel, Pierre; Helms, Greg; Hiscox, Bill
- Algal Research, Vol. 4
Cultivation of Nannochloropsis salina using anaerobic digestion effluent as a nutrient source for biofuel production
journal, August 2013
- Cai, Ting; Park, Stephen Y.; Racharaks, Ratanachat
- Applied Energy, Vol. 108
Cultivation of Chlorella zofingiensis in bench-scale outdoor ponds by regulation of pH using dairy wastewater in winter, South China
journal, October 2012
- Huo, Shuhao; Wang, Zhongming; Zhu, Shunni
- Bioresource Technology, Vol. 121
The roles of free ammonia (FA) in biological wastewater treatment processes: A review
journal, February 2019
- Liu, Yiwen; Ngo, Huu Hao; Guo, Wenshan
- Environment International, Vol. 123
Cultivation of Chlorella vulgaris JSC-6 with swine wastewater for simultaneous nutrient/COD removal and carbohydrate production
journal, December 2015
- Wang, Yue; Guo, Wanqian; Yen, Hong-Wei
- Bioresource Technology, Vol. 198
Cultivation of Green Algae Chlorella sp. in Different Wastewaters from Municipal Wastewater Treatment Plant
journal, November 2009
- Wang, Liang; Min, Min; Li, Yecong
- Applied Biochemistry and Biotechnology, Vol. 162, Issue 4
Aqueous Ammonia Equilibrium Calculations: Effect of pH and Temperature
journal, December 1975
- Emerson, Kenneth; Russo, Rosemarie C.; Lund, Richard E.
- Journal of the Fisheries Research Board of Canada, Vol. 32, Issue 12
Local bioprospecting for high-lipid producing microalgal strains to be grown on concentrated municipal wastewater for biofuel production
journal, July 2011
- Zhou, Wenguang; Li, Yecong; Min, Min
- Bioresource Technology, Vol. 102, Issue 13
Low-temperature anaerobic digestion for wastewater treatment
journal, June 2012
- McKeown, Rory M.; Hughes, Dermot; Collins, Gavin
- Current Opinion in Biotechnology, Vol. 23, Issue 3
Microalgal mass culture systems and methods: Their limitation and potential
journal, August 2001
- Lee, Yuan-Kun
- Journal of Applied Phycology, Vol. 13, Issue 4, p. 307-315
Co-liquefaction of swine manure and mixed-culture algal biomass from a wastewater treatment system to produce bio-crude oil
journal, September 2014
- Chen, Wan-Ting; Zhang, Yuanhui; Zhang, Jixiang
- Applied Energy, Vol. 128
Characterization of a microalga Chlorella sp. well adapted to highly concentrated municipal wastewater for nutrient removal and biodiesel production
journal, April 2011
- Li, Yecong; Chen, Yi-Feng; Chen, Paul
- Bioresource Technology, Vol. 102, Issue 8
Mitigating excessive ammonia nitrogen in chicken farm flushing wastewater by mixing strategy for nutrient removal and lipid accumulation in the green alga Chlorella sorokiniana
journal, May 2020
- Cui, Hongli; Ma, Haotian; Chen, Shuaihang
- Bioresource Technology, Vol. 303
Effects of solution conditions on the precipitation of phosphate for recovery
journal, September 2002
- Song, Yonghui; Hahn, Hermann H.; Hoffmann, Erhard
- Chemosphere, Vol. 48, Issue 10
Growth and nutrient removal in free and immobilized green algae in batch and semi-continuous cultures treating real wastewater
journal, January 2010
- Ruiz-Marin, Alejandro; Mendoza-Espinosa, Leopoldo G.; Stephenson, Tom
- Bioresource Technology, Vol. 101, Issue 1
Energetics of advanced integrated wastewater pond systems
journal, June 1995
- Green, F. B.; Lundquist, T. J.; Oswald, W. J.
- Water Science and Technology, Vol. 31, Issue 12
Tolerance and nutrients consumption of Chlorella vulgaris growing in mineral medium and real wastewater under laboratory conditions
journal, February 2017
- María de Lourdes, Franco Martínez; María Dolores Josefina, Rodríguez Rosales; Cuauhtémoc Ulises, Moreno Medina
- Open Agriculture, Vol. 2, Issue 1
Net Energy and Greenhouse Gas Emission Evaluation of Biodiesel Derived from Microalgae
journal, October 2010
- Batan, Liaw; Quinn, Jason; Willson, Bryan
- Environmental Science & Technology, Vol. 44, Issue 20
Growth and biochemical composition of the diatom Chaetoceros cf. wighamii brightwell under different temperature, salinity and carbon dioxide levels. I. Protein, carbohydrates and lipids
journal, May 2005
- de Castro Araújo, Sirlei; Garcia, Virgínia Maria Tavano
- Aquaculture, Vol. 246, Issue 1-4
Ammonia Triggers Photodamage of Photosystem II in the Cyanobacterium Synechocystis sp. Strain PCC 6803
journal, March 2008
- Drath, Miriam; Kloft, Nicole; Batschauer, Alfred
- Plant Physiology, Vol. 147, Issue 1
Mitigating ammonia nitrogen deficiency in dairy wastewaters for algae cultivation
journal, February 2016
- Lu, Qian; Zhou, Wenguang; Min, Min
- Bioresource Technology, Vol. 201
The Influence of Anaerobic Digestion Effluents (ADEs) Used as the Nutrient Sources for Chlorella sp. Cultivation on Fermentative Biogas Production
journal, August 2016
- Dębowski, Marcin; Szwaja, Stanisław; Zieliński, Marcin
- Waste and Biomass Valorization, Vol. 8, Issue 4
Carbon-dependent alleviation of ammonia toxicity for algae cultivation and associated mechanisms exploration
journal, February 2018
- Lu, Qian; Chen, Paul; Addy, Min
- Bioresource Technology, Vol. 249
Quantification of nitrification and denitrification rates in algae and duckweed based wastewater treatment systems
journal, March 2004
- Zimmo, O. R.; Van Der Steen, N. P.; Gijzen, H. J.
- Environmental Technology, Vol. 25, Issue 3
Growth and nutrient removal in free and immobilized planktonic green algae isolated from pig manure
journal, April 2004
- Jiménez-Pérez, M. V.; Sánchez-Castillo, P.; Romera, O.
- Enzyme and Microbial Technology, Vol. 34, Issue 5
The effect of bacterial contamination on the heterotrophic cultivation of Chlorella pyrenoidosa in wastewater from the production of soybean products
journal, November 2012
- Zhang, Yalei; Su, Hongyang; Zhong, Yunna
- Water Research, Vol. 46, Issue 17
Semi-continuous Cultivation of Chlorella vulgaris for Treating Undigested and Digested Dairy Manures
journal, June 2010
- Wang, Liang; Wang, Yingkuan; Chen, Paul
- Applied Biochemistry and Biotechnology, Vol. 162, Issue 8
Ammonium Nitrogen Tolerant Chlorella Strain Screening and Its Damaging Effects on Photosynthesis
journal, January 2019
- Wang, Jie; Zhou, Wei; Chen, Hui
- Frontiers in Microbiology, Vol. 9
Mixotrophic cultivation of Chlorella pyrenoidosa with diluted primary piggery wastewater to produce lipids
journal, January 2012
- Wang, Haiying; Xiong, Hairong; Hui, Zhenglong
- Bioresource Technology, Vol. 104
Exploiting mixotrophy for improving productivities of biomass and co-products of microalgae
journal, September 2019
- Pang, Na; Gu, Xiangyu; Chen, Shulin
- Renewable and Sustainable Energy Reviews, Vol. 112
High ammonia tolerance on growth rate of marine microalga Chlorella vulgaris
journal, September 2018
- Goto, M.; Nagao, N.; Yusoff, F. Md.
- Journal of Environmental Biology, Vol. 39, Issue 5(SI)
Life Cycle Environmental Impacts of Wastewater-Based Algal Biofuels
journal, September 2014
- Mu, Dongyan; Min, Min; Krohn, Brian
- Environmental Science & Technology, Vol. 48, Issue 19
Micro-algae as a source of protein
journal, March 2007
- Becker, E. W.
- Biotechnology Advances, Vol. 25, Issue 2
Synergistic integration of electrocoagulation and algal cultivation to treat liquid anaerobic digestion effluent and accumulate algal biomass
journal, January 2016
- Liu, Zhiguo; Liu, Yan
- Process Biochemistry, Vol. 51, Issue 1
Two-stage heterotrophic and phototrophic culture strategy for algal biomass and lipid production
journal, January 2012
- Zheng, Yubin; Chi, Zhanyou; Lucker, Ben
- Bioresource Technology, Vol. 103, Issue 1
Microalgae for biodiesel production and other applications: A review
journal, January 2010
- Mata, Teresa M.; Martins, António A.; Caetano, Nidia. S.
- Renewable and Sustainable Energy Reviews, Vol. 14, Issue 1, p. 217-232
Renewable biomass production by mixotrophic algae in the presence of various carbon sources and wastewaters
journal, October 2011
- Bhatnagar, Ashish; Chinnasamy, Senthil; Singh, Manjinder
- Applied Energy, Vol. 88, Issue 10
Neochloris oleoabundans grown on anaerobically digested dairy manure for concomitant nutrient removal and biodiesel feedstock production
journal, January 2011
- Levine, Robert B.; Costanza-Robinson, Molly S.; Spatafora, Grace A.
- Biomass and Bioenergy, Vol. 35, Issue 1
Nitrogen availability influences phosphorus removal in microalgae-based wastewater treatment
journal, June 2015
- Beuckels, Annelies; Smolders, Erik; Muylaert, Koenraad
- Water Research, Vol. 77
Effects of C5 organic carbon and light on growth and cell activity of Haematococcus pluvialis under mixotrophic conditions
journal, January 2017
- Pang, Na; Chen, Shulin
- Algal Research, Vol. 21
Chlorella sorokiniana UTEX 2805, a heat and intense, sunlight-tolerant microalga with potential for removing ammonium from wastewater
journal, July 2008
- de-Bashan, Luz E.; Trejo, Adan; Huss, Volker A. R.
- Bioresource Technology, Vol. 99, Issue 11
Cultivation of Chlorella vulgaris in Dairy Wastewater Pretreated by UV Irradiation and Sodium Hypochlorite
journal, October 2013
- Qin, Lei; Shu, Qing; Wang, Zhongming
- Applied Biochemistry and Biotechnology, Vol. 172, Issue 2
Integrated CO2 capture, wastewater treatment and biofuel production by microalgae culturing—A review
journal, November 2013
- Razzak, Shaikh A.; Hossain, Mohammad M.; Lucky, Rahima A.
- Renewable and Sustainable Energy Reviews, Vol. 27
Ammonia inhibition in oleaginous microalgae
journal, November 2016
- Gutierrez, James; Kwan, Thomas A.; Zimmerman, Julie B.
- Algal Research, Vol. 19
Nutrient recovery from wastewater streams by microalgae: Status and prospects
journal, March 2013
- Cai, Ting; Park, Stephen Y.; Li, Yebo
- Renewable and Sustainable Energy Reviews, Vol. 19
Assessment of photosynthesis regulation in mixotrophically cultured microalga Chlorella sorokiniana
journal, November 2016
- Li, Tingting; Kirchhoff, Helmut; Gargouri, Mahmoud
- Algal Research, Vol. 19
The IWA Anaerobic Digestion Model No 1 (ADM1)
journal, May 2002
- Batstone, D. J.; Keller, J.; Angelidaki, I.
- Water Science and Technology, Vol. 45, Issue 10
Scale-up potential of cultivating Chlorella zofingiensis in piggery wastewater for biodiesel production
journal, June 2013
- Zhu, Liandong; Wang, Zhongming; Takala, Josu
- Bioresource Technology, Vol. 137
Optimizing Cultivation Strategies for Robust Algal Growth and Consequent Removal of Inorganic Nutrients in Pretreated Livestock Effluent
journal, August 2014
- Kim, Hyun-Chul; Choi, Wook Jin; Ryu, Jun Hee
- Applied Biochemistry and Biotechnology, Vol. 174, Issue 4
Influence of microalgae cell wall characteristics on protein extractability and determination of nitrogen-to-protein conversion factors
journal, August 2012
- Safi, Carl; Charton, Michaël; Pignolet, Olivier
- Journal of Applied Phycology, Vol. 25, Issue 2