An atypical lipoteichoic acid from Clostridium perfringens elicits a broadly cross-reactive and protective immune response
Abstract
Clostridium perfringens is a leading cause of food-poisoning and causes avian necrotic enteritis, posing a significant problem to both the poultry industry and human health. No effective vaccine against C. perfringens is currently available. Using an antiserum screen of mutants generated from a C. perfringens transposon-mutant library, here we identified an immunoreactive antigen that was lost in a putative glycosyltransferase mutant, suggesting that this antigen is likely a glycoconjugate. Following injection of formalin-fixed whole cells of C. perfringens HN13 (a laboratory strain) and JGS4143 (chicken isolate) intramuscularly into chickens, the HN13-derived antiserum was cross-reactive in immunoblots with all tested 32 field isolates, whereas only 5 of 32 isolates were recognized by JGS4143-derived antiserum. The immunoreactive antigens from both HN13 and JGS4143 were isolated, and structural analysis by MALDI-TOF-MS, GC-MS, and 2D NMR revealed that both were atypical lipoteichoic acids (LTAs) with poly-(β1→4)-ManNAc backbones substituted with phosphoethanolamine. However, although the ManNAc residues in JGS4143 LTA were phosphoethanolamine-modified, a few of these residues were instead modified with phosphoglycerol in the HN13 LTA. The JGS4143 LTA also had a terminal ribose and ManNAc instead of ManN in the core region, suggesting that these differences may contribute to the broadly cross-reactive response elicited bymore »
- Authors:
-
[3];
[1];
[4];
- Univ. of Alberta, Edmonton, AB (Canada); VaxAlta Inc., Alberta (Canada)
- Univ. of Alberta, Edmonton, AB (Canada)
- Univ. of Georgia, Athens, GA (United States)
- Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States)
- VaxAlta Inc., Alberta (Canada); Washington Univ. of Medicine, St. Louis, MI (United States)
- Univ. of Alberta, Edmonton, AB (Canada); VaxAlta Inc., Alberta (Canada); Univ. of Georgia, Athens, GA (United States)
- Publication Date:
- Research Org.:
- Univ. of Georgia, Athens, GA (United States); Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1683628
- Alternate Identifier(s):
- OSTI ID: 1964030
- Grant/Contract Number:
- SC0015662
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Biological Chemistry
- Additional Journal Information:
- Journal Volume: 295; Journal Issue: 28; Journal ID: ISSN 0021-9258
- Publisher:
- American Society for Biochemistry and Molecular Biology
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; Clostridium perfringens; lipoteichoic acid (LTA); food safety; one health; NMR spectroscopy; cell surface; Gram-positive bacteria; necrotic enteritis; glycoconjugate vaccines; foodborne illness; glycoconjugate; glycobiology; glycolipid structure; cell wall; carbohydrate biosynthesis; carbohydrate structure; microbiology; microbial pathogenesis
Citation Formats
Wenzel, Cory Q., Mills, Dominic C., Dobruchowska, Justyna M., Vlach, Jiri, Nothaft, Harald, Nation, Patrick, Azadi, Parastoo, Melville, Stephen B., Carlson, Russell W., Feldman, Mario F., and Szymanski, Christine M. An atypical lipoteichoic acid from Clostridium perfringens elicits a broadly cross-reactive and protective immune response. United States: N. p., 2020.
Web. doi:10.1074/jbc.ra119.009978.
Wenzel, Cory Q., Mills, Dominic C., Dobruchowska, Justyna M., Vlach, Jiri, Nothaft, Harald, Nation, Patrick, Azadi, Parastoo, Melville, Stephen B., Carlson, Russell W., Feldman, Mario F., & Szymanski, Christine M. An atypical lipoteichoic acid from Clostridium perfringens elicits a broadly cross-reactive and protective immune response. United States. https://doi.org/10.1074/jbc.ra119.009978
Wenzel, Cory Q., Mills, Dominic C., Dobruchowska, Justyna M., Vlach, Jiri, Nothaft, Harald, Nation, Patrick, Azadi, Parastoo, Melville, Stephen B., Carlson, Russell W., Feldman, Mario F., and Szymanski, Christine M. Mon .
"An atypical lipoteichoic acid from Clostridium perfringens elicits a broadly cross-reactive and protective immune response". United States. https://doi.org/10.1074/jbc.ra119.009978. https://www.osti.gov/servlets/purl/1683628.
@article{osti_1683628,
title = {An atypical lipoteichoic acid from Clostridium perfringens elicits a broadly cross-reactive and protective immune response},
author = {Wenzel, Cory Q. and Mills, Dominic C. and Dobruchowska, Justyna M. and Vlach, Jiri and Nothaft, Harald and Nation, Patrick and Azadi, Parastoo and Melville, Stephen B. and Carlson, Russell W. and Feldman, Mario F. and Szymanski, Christine M.},
abstractNote = {Clostridium perfringens is a leading cause of food-poisoning and causes avian necrotic enteritis, posing a significant problem to both the poultry industry and human health. No effective vaccine against C. perfringens is currently available. Using an antiserum screen of mutants generated from a C. perfringens transposon-mutant library, here we identified an immunoreactive antigen that was lost in a putative glycosyltransferase mutant, suggesting that this antigen is likely a glycoconjugate. Following injection of formalin-fixed whole cells of C. perfringens HN13 (a laboratory strain) and JGS4143 (chicken isolate) intramuscularly into chickens, the HN13-derived antiserum was cross-reactive in immunoblots with all tested 32 field isolates, whereas only 5 of 32 isolates were recognized by JGS4143-derived antiserum. The immunoreactive antigens from both HN13 and JGS4143 were isolated, and structural analysis by MALDI-TOF-MS, GC-MS, and 2D NMR revealed that both were atypical lipoteichoic acids (LTAs) with poly-(β1→4)-ManNAc backbones substituted with phosphoethanolamine. However, although the ManNAc residues in JGS4143 LTA were phosphoethanolamine-modified, a few of these residues were instead modified with phosphoglycerol in the HN13 LTA. The JGS4143 LTA also had a terminal ribose and ManNAc instead of ManN in the core region, suggesting that these differences may contribute to the broadly cross-reactive response elicited by HN13. In a passive-protection chicken experiment, oral challenge with C. perfringens JGS4143 lead to 22% survival, whereas co-gavage with JGS4143 and α-HN13 antiserum resulted in 89% survival. This serum also induced bacterial killing in opsonophagocytosis assays, suggesting that HN13 LTA is an attractive target for future vaccine-development studies.},
doi = {10.1074/jbc.ra119.009978},
journal = {Journal of Biological Chemistry},
number = 28,
volume = 295,
place = {United States},
year = {Mon May 18 00:00:00 EDT 2020},
month = {Mon May 18 00:00:00 EDT 2020}
}
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